Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal BERITA BIOLOGI
Joko Ridho Witono
Laboratory of Plant Chromosome and Gene Stock, Graduate School of Science, Hiroshima University 1 -4-3 Kagamiyama, Higashi-Hiroshima City 739-8526, Japan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
MODIFICATION OF DNA ISOLATION PROTOCOL FROM SILICA GEL DRIED-LEAF TISSUES OF Pinanga (PALMAE) Joko Ridho Witono; Katsuhiko Kondo
BERITA BIOLOGI Vol 8, No 2 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i2.2034

Abstract

DNA isolation is an important issue in molecular fields especially for specific plant group such as Pinanga (Palmae). Typically,leaflets structure of Pinanga consists of shiny leaflets as common species of palms and mottled leaflets. Pinanga javana and P.coronata form commonly the two types of leaflets. The high quality of DNA of Pinanga with the shiny leaflet is readily purified and that with the mottled leaflet needed DNA purification due to the presence of high polyphenolic compound. Application of the silica binding method was recommended to purify the template DNA. Modified DNA-isolation protocol is relatively quick, simple,least expensive, minimum equipments and chemicals required, and suitable for PCR and endonucleases digestion reaction.
Co-Authors Katsuhiko Kondo