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Harmastini Sukiman
Pusat Penelitian Bioteknologi-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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ENDOFIT Taxus sumatrana (Miquel) de Laubenfels DAN POTENSINYA DALAM MEMPRODUKSI SENYAWA BIOAKTIF SEBAGAI SUMBER ANTIOKSIDAN Harmastini Sukiman
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.750

Abstract

Taxus sumatrana (Miquel) de Laubenfels is known as an endanger forest tree species grown at Kerinci National Park in Jambi,Indonesia. This plant was known potential on producing taxol. Taxol is a bioactive compound, could be used as antibacterial agent and recently confirmed to cure cancer cells. For the first time in 1960, Arthur Barclay found the taxol compound from Taxus sp.(pacific yew). However to isolate the bioactive compound, huge amount of tree biomass is needed. Research on endophytes microbes which are isolated from inner tissue of Taxus sp. declared that those microbes have potential on producing antioxidant agent for drug discovery. Isolation and conservation of endophytes and selecting its potential is promising the novel of finding new drug that may be effective for treating the newly developing diseases in human. Fifteen isolates of Taxus endophytes have been successfully studied on their ability on producing antioxidant. The results showed that endophytes fungus isolates TsC-17, isolated from Taxus sumatrana grown in Cibodas Botanical Gardens-LIPI, could produce extracellular bioactive compound which performed activity of suppressing free radical, is significantly better compared to intracellular bioactive compound eventhough it is not as high as in vitamin C. The activity of suppressing free radical resulted from bioactive compound of endophyte fungus isolates TsC-17 was 20% for intracellular bioactive compound, while it was 60% for extracellular bioactive compound and 90% for vitamin C.
RESPON TANAMAN PADI GOGO (Oryza sativa L.) TERHADAP STRESS AIR DAN INOKULASI MIKORISA Harmastini Sukiman; Adiwirman Adiwirman; Syofiatin Syamsiyah
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1979

Abstract

It has been known that upland rice production is lower than low land rice production because it is inhibited by soil fertility, water supply and pest.One solutions to increase the production of upland rice is by applying the potential soil microorganism known as mycorrhizae.The objective of this research is to know thef Vesicular Arbuscular Mycorrhizae (VAM) role for upland rice under water stress condition. The research was conducted in greenhouse of Cikabayan, Bogor Agricultural University and Soil Microbiology Laboratory of Research Centre of Biotechnology, Indonesian Institute of Science Centre. The experimental design was factorial randomized block design with two factors. The first factor is inoculation of mycorrhizae consisted of two treatment i.e. without mycorrhizae (MO) and with mycorrhizae (Ml); while the second factor is water stress regime consisted of five treatments namely well watered (SO), water stress on tillering stage (SI), primordial stage (S2), anthesis stage(S3) and grain filling stage(S4). Mycorrhizae significantly increased root infection, phosphate uptake, height, number of tillering, leaf, Leaf Area Index (LAI), productive tillering, filled grain, grain weight and yield. While, water stress significantly decreased number of root infection, shoot wet fresh weight, root dry weight, increased phosphate uptake and sterilized grain, but it didn't significantly decrease yield. The interaction both mycorrhizae and water stress didn't significantly influence all variables, except the root dry weight. The inoculation of mycorrhizae could increase 19.62% on SI, 17.32% on S2, 29.14% on S3, 6.89% on S4 dried harvested yield.
MIKROBAENDOFITIK DARI TAMAN NASIONALBATANG GADIS SUMATERA UTARA: POTENSINYA DALAM MENGHASILKAN SENYAWA ANTIMIKROBATERHADAPMIKROBAPATOGEN Harmastini Sukiman; Sylvia Lekatompessy; Tiwit Widowati
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i6.858

Abstract

Batang Gadis National Park (TNBG) is one of the tropical forests in North Sumatra with megabiodiversity of flora and fauna including microorganism. Endophytic microbes conservation from varieties of forest plants in Batang Gadis National Park has been completed. Nineteen endophytes isolates from TNBG have been screened for their potential on producing bioactive compound against the pathogenic bacteria. Qualitative screening has been done using the growth agar media and identified the clear zone appeared surrounding the bacteria colony. The result indicated that isolate MSCI 87.4 showed high strengthening secresion (4.35) againts Xanthomonas campestris, whereas isolate MSCI 37.1 showed high secretion against Bacillus subtilis (2.69) and Escherichia coli (2.60). Isolate MSCI 37.4 showed potential on producing bioactive compound againts Staphylococcus aureus (4.41). Isolates MSCI 87.4, MSCI37.1, MSCI 37.4 and MSCI 58.1 even could produce bioactive compound against four pathogenic bacteria that are Xanthomonas campestris, Bacilus subtilis, Escherichia coli and Staphylococcus aureus, whereas isolate MSCI 15.5b potential on producing bioactive compound against two isolates namely Xanthomonas campestris and Bacillus subtilis. The endophytes bacteria mainly belong to the Gram negative group and four out of nineteen isolates tested belong to the Gram positive group. The cell mainly coccus and only one is bacilli without fiagella. The five most potential isolates has been maintained under freeze dried condition for futher conservation and study. Thin Layer Analysis using semipolar organic separation solution showed that most isolates identified were able to produce bioactive compound except two isolates i.e. MSCI 48.4a and MSCI 53.1; however further analysis is needed to confirm the product.
Co-Authors Adiwirman Adiwirman Sylvia Lekatompessy Syofiatin Syamsiyah Tiwit Widowati