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Nunik Sulistinah
Bidang Mikrobiologi, Pusat Penelitian Biologi-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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KERAGAMAN BAKTERI PENGHASIL ENZIM PENGHIDROLISIS NITRIL DI PULAU ENGGANO BENGKULU Rini Riffiani; Nunik Sulistinah
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i3.2243

Abstract

Potential nitrile degrading microbes have been isolated from marine sponge, marine water and soil in Enggano Island. Nitrilase enzyme has a function in degrading nitrile compund. Nitrilases are important industrial enzymes because of their ability to produce biologically active to degrade enantiomers, such as S-(+)-1-(4’-isobutylphenyl) propionic acid (S-(+)-ibuprofen) and R-(-) mandelic acid. Mandelic acids, which are important as pharmaceutical intermediates, can be produced in enantiomerically pure form by the hydrolysis of their corresponding nitrile. The aim of the study was to explore the diversity of nitrile degrading bacteria in Enggano Island, and their ability to utilize nitrile as a substrate growth. Screening of such microbes were carried out by using microtitter plate method based on growth ability tested by INT (Iodonitrotetrazoliumchloride). Degradation product was determined by High Performance Liquid Chromatography (HPLC). Seventy nine bacteria were able to grow on acetamide, acetonitrile, benzonitrile, adiponitrile, mandelonitrile, succinonitrile, lactonitrile, dan benzilcyanide as the sole source of carbon and nitrogen. Two isolates, YIM 56238 and PO69, have shown to enantioselectively hydrolyze racemic mandelonitrile to mandelic acid. Based on 16S rRNA gene identification, these bacteria have the highest sequence similarity to Microccous endophyticus strain YIM 56238 and Rhodococcus pyridinivorans strain PO69.
KINETIKA BIOTRANSFORMASI SUKSINONITRIL OLEH Pseudomonas sp. Nunik Sulistinah; Bambang Sunarko
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2054

Abstract

Succinic acid (HOOC(CH2)2COOH) is commercial compound that primarily used in food products. This compound also used in textile industry for colouring process, drugs industry, paint, pernist and photography slice. Succinic acid usually produced in industry by chemical reaction and produce cyanide acid as by product. The objective of this research was to study the utilization of Pseudomonas sp. in produce succinic acid and to characterize of involved enzymes. The results showed that the enzyme optimally produce in log phase. The production rate of succinic acid formation was 0,982 mM/ml.h with the decrease of substrate consumption was 1,235 mM/ml.h. The yield of succinic acid during 81 hours fermentation was 81%. The optimum pH, temperature, Km and Vran were 7, 27°C, 90 mM, and 0,0002 mM/ml.h.
Co-Authors Bambang Sunarko Rini Riffiani