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Rudhy Gustiano
Balai Riset Perikanan Budidaya Air Tawar

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KARAKTERISASI 17 FAMILI IKAN NILA {Oreochromis niloticus) GENERASI KE TIGA (G-3) BERDASARKAN METODE TRUSS MORFOMETRIKS Nuryadi Nuryadi; Otong Zenal Ariiin; Rudhy Gustiano; Mulyasari Mulyasari
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.806

Abstract

Objective of the study is to elucidate phenotype characters of nile tilapia (Oreochromis niloticus) of the third generation (G3)resulted from the selection breeding program in the Research Institute for Freshwater Aquaculture Bogor. Phenotypes of seventeen nile tilapia families, were observed using truss morphometric methods. The results showed that the composition average of standard lenght has low coefficient variation mean (10.42%) as well as coefficient variation of the truss measured characters (4.3- 13.3%). The highest index similarity of boddy shape within family was found in family 5 (79.3%), in contrast the lowest was in family 12 (32.3%). For index similarity between families, the highest score was in between family 12 and 17 (22.6).There were 8 characters enabled to differentiate the 17 families observed. They were Al, A3, A5, B2, C3, C5, C5, and D4. Cluster analyses recognized into 4 groups based on the phenotypic distance
KERAGAMAN GENETIK POPULASI IKAN NILA {Oreochromis niloticus) DALAM PROGRAM SELEKSI BERDASARKAN RAPD Otong Zenal Arifin; Estu Nugroho; Rudhy Gustiano
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.826

Abstract

Objectives of the study was to discover genetic variability and genetic relationship of paternal half sib population of nile tilapia (Oreochromis niloticus) under Selection Program Scheme at Research Institute for Freshwater Aquaculture, in Bogor, West Java.Four populations from unrelated selected breeders were observed for genetic variability using RAPD. The analysis of amplification from each locus and fragments were used to estimate DNA polymorphisms, heterozygosity, fst and genetic distance.The range of heterozygosity of four examined populations was 0.1760-0.2168 with level polymorphic between 47.66% and 64.86%.The highest heterozygosity and polymorphic was on population 1 and the lowest one was on population 2. Fst test showed significance among the populations. The closest relationship was between population 1 and 4 (0.1978) and the furthest was population 2 and 3 (0.3289).