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Kusumadewi Sri Yulita
Pusat Penelitian Biologi-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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KERAGAMAN GENETIK BEBERAPA KLON DURIAN (Durio zibethinus Murray) ASAL JAWA BARAT BERDASARKAN SIDIK RANDOM AMPLIFIED POLIMORPHIC DNA Kusumadewi Sri Yulita; Muna Murnianjari
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.741

Abstract

Durian (Durio zibethinus) is one of the most popular tropical fruits in SE Asia. Indonesia has several local clones that have not yet been widely introduced to local fruit markets. This present study aimed to assess genetic diversity of 17 clones of durian from West Java based on RAPD fingerprints. Thirty RAPD's primers were initially screened and four were selected for the analysis.These four primers (OPA 13, OPD 8, OPN 6 and OPA 18) generated 63 scorable bands to which 100% of them were polymorphic.OPA-13 at 700bp was exclusively possessed by Tambleg clone and other bands were shared among the other clones. Clustering analysis was performed based on RAPD profiles using the UPGMA method. The range of genetic similarity value among genotypes was 0.15-0.73 suggesting high genetic variation among them. Results from genetic diversity analysis based on plant propagation system showed a higher genetic diversity value in occulating (87.30%) plants than that of grafting (60.32%).
RESPON TANAMAN JAGUNG VARIETAS LOKAL NTT UMUR SANGAT GENJAH (PENA TUNU’ ANA’) TERHADAP CEKAMAN KEKERINGAN  Tri Murningsih; Kusumadewi Sri Yulita; Charles Y. Bora; I.G.B. Adwita Arsa
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1865

Abstract

In dry land areas such as Nusa Tenggara Timur (NTT), low production of maize may due to selection of using local varieties that are mostly drought tolerant.One of the local varieties known as Pena Tunu’ Ana’ have been harversted in very early age of 1.5 month, hence it was categorised as very early mature maize. This variety is therefore play an important role in the food security system for people in NTT as they can be harvested earlier than other varieties. However, no information has yet on how this variety response to drought stress. Drought stress is one of the factors causing the decline in maize production. This study was conducted at experimental house in Cibinong Science Centre (CSC) to evaluate responses of Pena Tunu’ Ana’ against drought stress treatment. The experiment was done in six degree of drought treatments, 0 (control), 4, 8, 12, 16 and 20 days with 4 replications. Parameters measured were soil water, relative leaf water,proline and sugar content in leaves and roots at each level of drought stressed. Results showed that with increasing level of drought stress,soil water and relative leaf water content were decreased while proline and sugar content were increased. At the same level of drought stress, accumulation of proline and sugar in the roots was higher than those in the leaves. Drought stress terminated at 16 days, where the roots content of proline reached 10.36 mg/g and sugar 110.91 mg/g, while the leaves content of proline was of 2.84 mg/g and sugar was of 38.44 mg/g. At the level of drought stress during 16 days, the plants suffered temporary wilting but refreshed after watering.
Co-Authors Charles Y. Bora I.G.B. Adwita Arsa Muna Murnianjari Tri Murningsih