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Journal : BERITA BIOLOGI

PENINGKATAN PERFORMA REPRODUKSI IKAN PATIN SIAM (Pangasianodon hypophthalmus) PADA MUSIM KEMARAU MELALUI INDUKSI HORMONAL Evi Tahapari; Raden Roro Sri Pudji Sinarni Dewi
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.533

Abstract

Striped catfish (Pangasianodon hypophthalmus) spawning success is strongly influenced by environment.Striped catfish spawning generally occurs during the rainy season while in the dry season is difficult to find mature female. This research was conducted to improve reproduction performance of female striped catfish during dry season through combination injection of hormone pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG).The treatments were A : control (no hormone injections), B : injection of PMSG 10 IU/kg + HCG 10 IU/kg and C : injection of PMSG 20 IU/kg + HCG 10 IU/kg.Injections were done at interval of two weeks as many as six times. The research was conducted during dry season in June-October 2012 with rainfall 0.20- 14.40 mm/years. The results showed combination injection of PMSG + HCG could improve female reproduction performance. The best treatment was shown on C treatment with values of OSI (ovi somatic index), fecundity, fertilization rate, hatching rate and larva production was 12.18 %, 523 000 eggs/female, 79.65 %, 69.93 % and 290 000 larvae/female respectively. As for control treatment (A) the value of OSI, fecundity, fertilization rate, hatching rate and larval production, was 6.45 %, 197 000 eggs/female, 48.55 %, 35.59 % and 34 000 larvae/female, respectively
PERKEMBANGAN EMBRIO DAN LARVA IKAN PATIN NASUTUS (Pangasius nasutus Bleeker, 1863) (Pangasiidae; Pisces) Bambang Iswanto; Evi Tahapari
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.622

Abstract

Patin nasutus (Pangasius nasutus Bleeker, 1863) (Pangasiidae; Pisces) is a potential pangasiid catfish species to be cultured in Indonesia.Many efforts has been done to explore the potency of P.nasutus for aquaculture purposes.However, basic knowledge is still very rare such as its embryonic and larval development.The present study aimed to understand the embryonic and larval development of P. nasutus resulted from induced spawning of P.nasutus brooders at Research Institute for Fish Breeding, Sukamandi. Those embryonic and larval development were observed microscopically.The results showed that diameter of the ovulated oocytes ranged 1.40-1.65 mm and fertilized eggs ranged 1.90-2.15 mm.The embryogenesis consisted of several stages as follow: morula, blastula, gastrula, organ developments and newly hatched larvae occured within periods of (minutes) 20-200, 190-220, 210-660, 600-1,140 and 1,800-2,160 after fertilization at water temperature of 28-29oC. Total length of the newly hatched larvae ranged 4.80-5.10 mm with anterior, posterior and dorsal parts of the yolk sac coloured black pigmentation. On caudal peduncle, the black spot existed since 36 hours after hatching was a strong character for P.nasutus larvae.Volume of the yolk sac was 2.65±0.14 mm3, for which 50% absorbed at 36 hours after hatching and relative fully absorbed at 78 hours after hatching. The larvae have complete morphology as adult after seven days. During early developmental stage, larvae showed cannibalism since the third day, corresponding to the first exogenous feeding.