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Bambang Iswanto
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PERKEMBANGAN OOSIT IKAN PATIN SIAM, Pangasianodon hypophthalmus Sauvage, 1878 (PANGASIIDAE; SILURIFORMES) Evi Tahapari; Bambang Iswanto
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.748

Abstract

Pangasianodon hypophthalmus is an introduced species of fish culture and fisheries resources in Indonesia. Reproductive biology aspects, e.g. maturity, plays an important role on the fisheries management. There was no detail information of oocytes development of P. hypophthalmus. The aim of this study is to find out the detail of its oocytes development through the observation of whole oocytes and ovarian histological slides microscopically. The results showed that the rhythm of oocyte development of P. hypophthalmus was grouped synchronism, assigned by the presence of two oocyte groups in the mature females, i.e. the large and maturing oocytes which would be spawned soon, and the small ones as the stock and still unyolked for the next spawning. The oocyte development could be devided into five stages, i.e. stage 1 (chromatin nucleolar and perinucleolar) with oocytes diameter less than 300 urn, stage 2 (yolk vesicles and cortical alveolar) with oocytes diameter of 230-660 urn, stage 3 (yolk granules) with oocytes diameter of 430-1,100 urn, stage 4 (migratory nucleus and hydrated) with oocytes diameter of 950-1,260 urn, and stage S (atretic) with oocytes diameter less than 800 m
Persentase Post Larva Udang Galah (Macrobrachium rosenbergii) Dengan Pemberian L-Ascorbyl-2-Monophosphate-Magnesium Dalam Air Raden Roro Sri Pudji Sinarni Dewi; Bambang Iswanto
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.181 KB) | DOI: 10.22146/jfs.45

Abstract

The aim of this study was to improve the production homogeneity of freshwater prawn post larva by the addition of L-ascorbyl-2-monophosphate-Mg (AMP-Mg) in the water during rearing period. AMP-Mg was given to the water every three days with different concentrations of 0; 0.5; 1.0; and 1.5 mg/l. The density of larvae was 50 larvae/l. Larvae were fed Artemia and egg custard during rearing period. The results showed that AMP-Mg did not significantly influence larval metamorphose rate, post larvae percentage, and survival rate of prawn. However, AMP-Mg addition could improve homogeneity of post larvae production. The optimum AMP-Mg concentration was 0.5 mg/l.
PERKEMBANGAN OVARI DAN OOSIT IKAN PATIN HASIL PERSILANGAN ANTARA BETINA PATIN JAMBAL (Pangasius djambal Bleeker) DENGAN JANTAN PATIN SIAM (Pangasianodon hypophthalmus Sauvage) Evi Tahapari; Bambang Iswanto
Jurnal Perikanan Universitas Gadjah Mada Vol 10, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8884

Abstract

The aim of the study was to obtain the information of the gonadal development i.e ovary and oocyte of the hybrid between female patin jambal (Pangasius djambal) and male patin siam (Pangasianodon hypophthalmus). The gonadal development was investigated monthly by dissection of fish samples with 225.2-2,238.6 grams of body weight. Observations were carried out visual-macroscopically for shape, colour, and size of the ovaries. The visual-microscopically observations were carried out for both whole oocyte and ovarian histological slides. The results showed that the ovaries were very small, look alike short lobes. Its colour were grey-redish, very fatly, some of which were rudimentary, with gonadosomatic indices (GSI) ranged 0.05-0.92%. The most advanced stage of ovarian development was stage II (developing stage), later developed as regression ovary with major atresia, and never reached mature stage. The intraovarian oocytes developed as stage 1 (chromatin nucleolar and perinucleolar stages) with less than 225 μm in diameter and stage 2 (cortical alveolar and yolk vesicles stages) with 200- 875 µm in diameter, and the most advanced oocyte stage reached stage 2 only. There were no oocytes reached vitellogenesis stage (stage 3, yolk granules stage) neither mature oocyte stage (stage 4, migratory nucleus and hydrated oocyte stages), and showed major atresia, assigned by presence of the atretic oocyte stage (stage 5).
Co-Authors Evi Tahapari Raden Roro Sri Pudji Sinarni Dewi