Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal BERITA BIOLOGI Menara Perkebunan
Rizkita Rahmi Esyanti
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
POLA PRODUKSI AJMALISIN DARIKULTUR AGREGAT SEL Catharanthus roseus (L) G Don. DALAM BIOREAKTOR Aida Muspiah; Rizkita Rahmi Esyanti; Arbayah H Siregar; Erman Tritama
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1212

Abstract

A research has been conducted to optimize the rate of aeration and initial cell aggregates weight in the production of ajmalicine in Catharanthus roseus cell culture in bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.5 x 10"6 M NAA ('naphthalene acetic acid') and 10s M BAP ('benzyl amino purine'). Cell aggregates were subcultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with the rate of 0.2460 L/min and 0,3405 L/min, respectively. The pattern of ajmalicine production in bioreactor were observed every 3 days for 24 days.Qualitative and quantitative analysis were conducted using HPLC connected to Cromatopac CL-7APlus. The results showed that the cell aggregates and medium contain ajmalicine. The highest concentration was obtained in combination of 30 g/fw and 0.3405 L/min aeration compare to 20 g/fw - 0,246 L/min, 20 g/fw- 0,3405 L/min, as well as 30 g/fw - 0,2460 L/min. The highest ajmalicine content in cell aggregates was obtained on the 12"d days (79.23ug/g) whilst in medium was obtained in the 18th days (981.15 ug/L).
Pengaruh kitosan terhadap peningkatan level ekspresi WRKY17 dan WRKY53 tanaman Capsicum annuum cv. Laba pada kondisi kekeringan Muhammad Abdul AZIZ; Rizkita Rahmi ESYANTI; Fenny Martha DWIVANNY
E-Journal Menara Perkebunan Vol 88, No 2 (2020): Oktober,2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i2.380

Abstract

Chitosan is known as the natural plant growth biostimulant and defense elicitor involving WRKY transcription factors in response to environmental stresses. However, either plant’s growth or defense responses against stress are different among the cultivars. In the previous study, chitosan and drought treatment combination on red chili plant cv. Lado resulted in the impeded growth performance followed by the down-regulated expression level of WRKY17. Hence, this study aimed to investigate the response of red chili plant from the different cultivar towards the combination of chitosan and drought treatments. At the onset of the generative phase, plants were subjected to the drought treatment with and without 1 mg mL-1 of chitosan application. Observation of plant growth performance was carried out by measuring plant height, the number of leaves, the conversion percentage of flowers developed to fruits, and the number of harvested fruits, while the molecular parameter was indicated by the analyses of WRKY17 and WRKY53 expression levels. In line with the previous study using cv. Lado, the combination of 1 mg mL-1 of chitosan and drought treatment significantly reduced the plant productivity observed in the number of fruits, followed by the plant height and the number of leaves. However, the expression level of WRKY17 and WRKY53 experienced 10 and 22-fold higher than controls, respectively. This is different from the previous study which showed the reduction of WRKY17 expression level. According to the study, it can be implied that the combination of chitosan and drought treatments on red chili plant cv. Laba could reduce plant growth performance, but increased genetics indicator towards plant’s defense system against stress indicated by the escalation of WRKY17 and WRKY53 expression level. Furthermore, the individual chitosan application are potential to increase the productivity of red chili plant cv. Laba. 
Pengaruh kitosan terhadap peningkatan level ekspresi WRKY17 dan WRKY53 tanaman Capsicum annuum cv. Laba pada kondisi kekeringan Muhammad Abdul AZIZ; Rizkita Rahmi ESYANTI; Fenny Martha DWIVANNY
Menara Perkebunan Vol. 88 No. 2 (2020): 88 (2), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i2.380

Abstract

Chitosan is known as the natural plant growth biostimulant and defense elicitor involving WRKY transcription factors in response to environmental stresses. However, either plant’s growth or defense responses against stress are different among the cultivars. In the previous study, chitosan and drought treatment combination on red chili plant cv. Lado resulted in the impeded growth performance followed by the down-regulated expression level of WRKY17. Hence, this study aimed to investigate the response of red chili plant from the different cultivar towards the combination of chitosan and drought treatments. At the onset of the generative phase, plants were subjected to the drought treatment with and without 1 mg mL-1 of chitosan application. Observation of plant growth performance was carried out by measuring plant height, the number of leaves, the conversion percentage of flowers developed to fruits, and the number of harvested fruits, while the molecular parameter was indicated by the analyses of WRKY17 and WRKY53 expression levels. In line with the previous study using cv. Lado, the combination of 1 mg mL-1 of chitosan and drought treatment significantly reduced the plant productivity observed in the number of fruits, followed by the plant height and the number of leaves. However, the expression level of WRKY17 and WRKY53 experienced 10 and 22-fold higher than controls, respectively. This is different from the previous study which showed the reduction of WRKY17 expression level. According to the study, it can be implied that the combination of chitosan and drought treatments on red chili plant cv. Laba could reduce plant growth performance, but increased genetics indicator towards plant’s defense system against stress indicated by the escalation of WRKY17 and WRKY53 expression level. Furthermore, the individual chitosan application are potential to increase the productivity of red chili plant cv. Laba. 
Co-Authors AIDA MUSPIAH Arbayah H Siregar Erman Tritama Fenny Martha DWIVANNY Muhammad Abdul AZIZ