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I Made Samudera
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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KARAKTERISASI ß-1,3-1,4-GLUKANASEBAKTERI ENDOFITIK Burkholderia cepacia ISOLATE76 ASAL TANAMAN PADI Ifa Manzila; Tri Puji Priyatno; Muhammad Faris Fathin; Laksmi Ambarsari; Yadi Suryadi; I Made Samudera; Dwi Ningsih Susilowati
BERITA BIOLOGI Vol 14, No 2 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i2.1819

Abstract

Pathogenic fungus is one of the constraints to increase crop production. Chemical control using fungicides caused negative effects either to the environment or increased pathogen resistance to fungicide. Biological control using microbial-producing ß glucanase is an alternative method to inhibit the growth of pathogenic fungus. The aim of this study was to characterize ß-1,3-1,4-glucanase produced by rice endophytic bacterium, B. cepacia E76. Purification was carried out by ammonium sulphate precipitation, dialysis, and ion exchange chromatography using DEAE sepharose Fast Flow. A further characteristic of the enzyme activity was studied using oatmeal-glucan substrate.Results showed that precipitation using saturated 80% ammonium sulphate generated a good yield with the purity increased by 11 fold and yield of 66%.After chromatography step, the ß-1,3-1,4-glucanase of B. cepacia was successfully purified with an increasedof purity up to 33 fold and yield of 4%. Based on 10% SDS-PAGE, the enzyme profiles had the molecular weight of 15, 48 and 55 kDa.Of the three isozymes, only the 48 kDa isozyme showed the strongest glucanase activity when grown on media containing glucan as substrate.
PENGKAJIAN PENERAPAN PENGENDALIAN HAMA ULAT, Spodoptera exiqua PADA USAHATANI BAWANG MERAH DI SERANG, BANTEN Resmayeti .; I Made Samudera
Jurnal Agroekoteknologi Vol 7, No 2 (2015)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.984 KB) | DOI: 10.33512/j.agrtek.v7i2.1072

Abstract

Pest is one of the limiting factors in shallots cultivation.To overcome pest problems,shallots farmers generally use pesticides intensively. These circumstance led theincrease of production costs and inefficient on shallots cultivation. One effort is toreduce production costs and the use of pesticides with the application of pest controlthreshold. Study of determination of control threshold on shallots cultivation wascarried out at Kramatwatu Subdistrict, Serang District, Banten Province, in Novemberuntil December 2014. Three treatments tested in the experiment, namely (A). S.exiqua moth caugth > 10 individu per day, (B). Plant damage of 5%, (C). application ofpestiscide every 3 days. The experiment used a randomiced block design and eachtreatment was repeated eight times. Results showed that control threshold based on thethe catch of the mouth > 10 per day gave shallots yields of 14.78 t/ha and benefit of Rp.99.780.000/ha with the value of B/C ratio of 2,07. In the treatment of B, based on cropdamage the yield was 12.20 t/ha and benefit of Rp 69.780.000/ha was obtained withB/C 1.38; while in treatment C, application of pesticides every 3 days yielded 11.40 t/haand gave benefit of Rp 62.080.000/ha with B/C at 1.19. Implementation on the controlthreshold, was economically feasible to adopted because it can inrease the yield and netbenefits compared to the system of pest routinely aplied every 3 days and plant damage5%. Key Words: Spodoptera exiqua, control threshold, benefits
Co-Authors DWI NINGSIH SUSILOWATI Ifa Manzila LAKSMI AMBARSARI Muhammad Faris Fathin Resmayeti . Tri Puji Priyatno Yadi Suryadi