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All Journal Dental Journal (Majalah Kedokteran Gigi)
Hendy Jaya Kurniawan
Faculty of Dental Medicine, Universitas Airlangga, Surabaya
Dentistry

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The effects of different 650 nm laser diode irradiation times on the viability and proliferation of human periodontal ligament fibroblast cells Kun Ismiyatin; Ari Subiyanto; Ika Tangdan; Rahmi Nawawi; Reinold C. Lina; Rizky Ernawati; Hendy Jaya Kurniawan
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 3 (2019): September 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i3.p142-146

Abstract

Background: Endo-perio lesions are clinical manifestations of inflammation in the periodontal and pulp tissue. Damage to the periodontal ligament can inhibit its ability to regenerate. Therefore, laser therapy use is expected to improve the prognosis with regard to healing lesions. Unfortunately, the duration of irradiation during laser diode therapy can influence the viability and proliferation of human periodontal ligament fibroblast (hPDLF) cells. Purpose: This study aims to determine the effects of different irradiation exposure times of the 650 nm laser diode of the pulsed mode type on the viability and proliferation of human periodontal ligament fibroblast cells. Methods: This study constituted a laboratory experiment on hPDLF cells using 650 nm laser diode irradiation. Six groups formed the research subjects in this study, namely; two control groups, two radiation groups respectively subjected to irradiation exposure of 15 seconds and 35 seconds duration followed by 24-hour incubation, and two radiation groups exposed to irradiation for 15 and 35 seconds respectively followed by 72-hour incubation period. The viability and proliferation of those cells were subsequently calculated by ELISA reader, while the data was analyzed by means of one-way ANOVA and Tukey tests. Results: The significance value of the viability scores between the 15-second irradiation group and the 35-second irradiation group was less than 0.05, indicating that there was a significant difference between these treatment groups. Similarly, the significance value of proliferation scores between the 15-second irradiation group and the 35-second irradiation group was less than 0.05, again indicating a significant difference between these treatment groups. Conclusion: Irradiation using a 650 nm laser diode 15 seconds and 35 seconds in duration can induce an increase in the viability and proliferation of hPDLF cells.
The expression of nuclear factor of activated T cell c1 and receptor activator of nuclear factor kappa β induced by Enterococcus faecalis in osteoclastogenesis (laboratory experiment on Wistar rats) Nirawati Pribadi; Rosita Rahmawati; Mandojo Rukmo; Adelina Kristanti Tandadjaja; Hendy Jaya Kurniawan; Ratna Puspita Hadi
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 4 (2019): December 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i4.p172-176

Abstract

Background: Enterococcus faecalis (E. faecalis) is the most common bacteria species in persistent endodontic infection of teeth undergoing root canal treatment at a prevalence of 38%. The virulence factor of this bacterium is Lipoteichoic acid (LTA) which can be recognized by Toll-like receptors-4 (TLR-4) that produce a stimulus and provoke an immune response. Inflammation results in bone defects that feature multiple cytokines and interactions between different cell types. Bone loss within a periapical tooth is characterized by osteoclast formation (osteoclastogenesis) in the bone. Purpose: This study aimed to determine the expression of nuclear factor of activated T cell c1 (NFATc1) and receptor activator of nuclear factor kappa β (RANK) which played a role in osteoclastogenesis at different time intervals. Methods: 36 upper molar teeth of the research subjects were induced with 106 CFU Enterococcus faecalis and subsequently observed for 7 and 21 days with the NFATc1 and RANK being counted microscopically at 1000X magnification across 20 viewing fields. Thereafter, the data was examined and analyzed by means of an independent T test using SPSS. Results: NFATc1 and RANK expression were higher in the group including E. faecalis on days 7 and 21 than in the control group. There were significant differences between the treatment group and control group with regard to NFATc1 and RANK expression (p<0.05). Conclusion: The study showed that the expression of NFATc1 and RANK, which plays a role in osteoclastogenesis, was higher in periapical bone defects in Wistar rats induced by E. faecalis than those which were not induced.
The aesthetic management of a midline diastema with direct composite using digital smile design, putty index and button shade technique: A case report Nirawati Pribadi; S. Sukaton; Galih Sampoerno; S. Sylvia; Hendy Jaya Kurniawan; Maya Safitri; Rahmadanty Mustika
Dental Journal (Majalah Kedokteran Gigi) Vol. 55 No. 1 (2022): March 2022
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v55.i1.p44-48

Abstract

Background: A diastema is the distance or space between two or more adjacent teeth. This abnormality can interfere with the aesthetics of a patient, and 97% of diastemas occur in the maxilla. Various treatments can be performed for diastema closure in patients, one of which is composite resin restoration. Purpose: To explain the aesthetic procedure for diastema closure. Case: A 20-year-old female patient presented with complaints of the distance between her anterior teeth (Class I Angle occlusion with normal overjet and overbite). The labial frenum associated with the diastema was normal in size and position. The patient was not amenable to invasive procedures. Case Management: Management of midline diastema closure using the direct composite technique with DSD, the putty index method and button shade technique. Conclusion: The closure of a midline diastema with direct composite using DSD, the putty index method and button shade technique provides aesthetic results with less cost and time due to the absence of laboratory procedures.
Co-Authors Adelina Kristanti Tandadjaja Ari Subiyanto Galih Sampoerno Ika Tangdan Kun Ismiyatin Mandojo Rukmo Maya Safitri Nirawati Pribadi Rahmadanty Mustika Rahmi Nawawi Ratna Puspita Hadi Reinold C. Lina Rizky Ernawati Rosita Rahmawati S. Sylvia Sukaton, Sukaton