Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS)
Angliana Chouw
Prodia Stemcell Indonesia, Jl. Kramat VII No.11, Jakarta
Biochemistry, Genetics & Molecular Biology Dentistry Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Growth and Osteogenic Differentiation of CD117+ Dental Pulp and Periodontal Ligament Cells Ferry Sandra; Janti Sudiono; Ciptadhi Tri Oka Binartha; Angliana Chouw; Melanie Sadono Djamil
The Indonesian Biomedical Journal Vol 9, No 2 (2017)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v9i2.286

Abstract

BACKGROUND: Dental pulp stem cell (DPSC) and periodontal ligament stem cell (PDLSC) have been suggested as valuable seed cells for bone engineering, suggesting that both stem cells are potential osteogenic sources. Since DPSC and PDLSC seem like to have similar potential in bone formation, we conducted a study to compare morphology, immunophenotype and cell growth of DPSC and PDLSC isolated from the same teeth.METHODS: Human dental pulps and periodontal ligaments were obtained from freshly extracted partial impacted third molar teeth. Collected samples were digested with type I collagenase. Resulted cell suspension was washed and cultured. For biomarker identification, the cells were fixed and bound with anti-fluorescein isothiocyanate (FITC)-cluster of differentiation (CD)117 antibody. For cell growth quantification, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used. Meanwhile for osteogenic differentiation, the cells were cultured in osteogenic medium for 1-3 weeks, fixed and stained with alizarin red.RESULTS: Morphology of dental pulps cell (DPC) and periodontal ligament cell (PDLC) in passage 5 was similar. Clear CD117 green fluorescence of DPC and PDLC in passage 5 was observed. Cell growth rate of PDLC was higher than the one of DPC, 0.3858 and 0.3848 respectively. DPC formed bone nodule on the third week culture in osteogenic medium, while PDLC showed bone nodule formation on the second week culture.CONCLUSION: We suggest that DPC and PDLC are potential seed cells for osteogenic regeneration, since they had cell growth capacity and osteogenic differentiation, particularly PDLC that had faster osteogenic differentiation.KEYWORDS: dental pulp, periodontal ligament, cell, growth, osteogenic differentiation
Investigation on Cell Surface Markers of Dental Pulp Stem Cell Isolated from Impacted Third Molar Based on International Society for Cellular Therapy Proposed Mesenchymal Stem Cell Markers Ferry Sandra; Janti Sudiono; Yohanna Feter; Nadhia Sari Afiana; Jessica Nathalia Chandra; Kharima Abdullah; Jasmine Shafira; Angliana Chouw
Molecular and Cellular Biomedical Sciences Vol 3, No 1 (2019)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1360.79 KB) | DOI: 10.21705/mcbs.v3i1.34

Abstract

Background: Recently we have isolated and cultured dental pulp stem cell (DPSC) derived from impacted third molar (DPSC-M3). The DPSC-M3 was suggested as mesenchymal stem cell, however the cell surface markers were not completely clarified. Therefore current study was conducted to investigate the markers.Materials and Methods: Passage 5 DPSC-M3 was cultured, labeled and examined with flow cytometer. All markers were investigated according to the proposed cell surface marker panel for the minimal identification of human mesenchymal stem cell (MSC) by International Society for Cellular Therapy (ISCT). The positive markers were cluster of differentiation (CD)90, CD73, CD105, while the negative markers were CD34, CD45, CD11b, CD19, and Human Leukocyte Antigen (HLA)-DR. Results: Results showed that the size and granularity of DPSC-M3 were ranged from 75 to 230 and 27 to 203, respectively. The cell surface antigens examination showed that CD90, CD105 and CD73 were highly expressed (>95%), meanwhile expressions of CD45, CD34, CD11b, CD19 and HLA-DR were <2%.Conclusion: Since the all markers expression were in accordance to the proposed cell surface marker panel for the minimal identification of human MSC by ISCT, DPSC-M3 could be suggested as an MSC.Keywords: dental pulp, stem cell, dental pulp stem cell, ISCT, flow cytometry
Co-Authors Ciptadhi Tri Oka Binartha Ferry Sandra Janti Sudiono Janti Sudiono Jasmine Shafira Jessica Nathalia Chandra Kharima Abdullah Melanie Sadono Djamil Nadhia Sari Afiana Yohanna Feter