Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Pelita Perkebunan BIOMA : Jurnal Biologi Makassar
Jaka Widada
Fakultas Pertanian, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
PENGHAMBATAN CYTHOPHATIC EFFECT (CPE) PADA SEL BHK-21 YANG TERINFEKSI VIRUS DENGUE SEROTIPE 4 (DENV-4) DENGAN PEMBERIAN EKSTRAK N-HEKSAN STREPTOMYCES SP. GMR22 Diani Mentari; Jaka Widada; Tri Wibawa; Nastiti Wijayanti
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 2 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i2.10292

Abstract

Negara tropis seperti Indonesia sangat berpotensi sebagai tempat berkembangnya berbagai penyakit menular berbahaya salah satunya Demam Berdarah Dengue (DBD) yang disebabkan oleh infeksi virus dengue. Variasi genetik pada serotipe virus dengue menyebabkan penyakit ini menjadi perhatian dunia medis karena menyebabkan kompleksitas respon imun yang berbeda. Vaksin Dengue (Denvaxia) diperkenalkan oleh WHO pada akhir tahun 2015, namun penggunaannya belum sepenuhnya efektif. Hal ini menyebabkan banyak peneliti berupaya untuk mencari senyawa bioaktif yang memiliki potensi untuk dikembangkan sebagai agen antiviral. Streptomyces sp. GMR22 diketahui menghasilkan senyawa bioaktif dengan spektrum yang luas. Ekstrak n-heksane Streptomyces sp. GMR22 memiliki nilai CC50 yang tidak terlalu tinggi dibandingkan dengan ekstrak Etil Asetat. Hal ini menyebabkan metabolit sekunder yang dihasilkan dapat digunakan untuk analisis lanjut seperti uji antivirus karena aman terhadap sel BHK-21 yang merupakan host virus dengue. Virus DENV-4 merupakan serotipe endemik di Asia Tenggara. Penelitian ini bertujuan untuk mengetahui aktivitas antivirus DENV-4 menggunakan ekstrak n-heksan Streptomyces sp. GMR22. Aktivitas antivirus dilakukan melalui pengamatan sel BHK-21. Hasil pengamatan morfologi sel BHK-21 terinfeksi virus DENV-4 menunjukkan bahwa pemberian ekstrak n-heksan Streptomyces sp. GMR22 mengurangi terbentuknya Cythophatic Effect (CPE). Namun penggunaan konsentrasi 40 µg/mL menyebabkan kematian pada sel BHK-21. Kata kunci:  antiviral, DENV-4, metabolit sekunder,  Streptomyces sp. GMR22, CPE
Ecosystem Service of Shade Trees on Nutrient Cycling and Productivity of Coffee Agro-ecosystems Rusdi Evizal; Tohari Tohari; Irfan D. Prijambada; Jaka Widada; Donny Widianto
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 25 No 1 (2009)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v25i1.108

Abstract

Shade trees are significant in certification scheme of sustainable coffee production. They play an importance role on ecosystem functioning. This research is aimed to study ecosystem service of shade trees in some coffee agro-ecosystems particularly on nutrient cycling and land productivity. Four agro-ecosys tems of Robusta coffee (Coffea canephora), namely sun coffee (without shade trees), coffee shaded by Michelia champaca, coffee shaded by Gliricidia sepium, and coffee shaded by Erythrina indica are evaluated during 2007—2008. Smallholder coffee plantation in Sumberjaya Subdistrict, West Lampung, which managed under local standard were employed using Randomized Complete Block Design with 3 replications. The result showed that litter fall dynamic from shade trees and from coffee trees was influenced by rainfall. Shade trees decreased weed biomass while increased litter fall production. In dry season, shade trees decreased litter fall from coffee shaded by M. champaca. G. sepium and E. indica shaded coffee showed higher yield than sun coffee and M. champaca shaded coffee. Except for M. champaca shaded coffee, yield had positive correlation (r = 0.99) with litter fall production and had negative correlation (r = —0.82) with weed biomass production. Biomass production (litter fall + weed) of sun coffee and shaded coffee was not significantly different. Litter fall of shade trees had significance on nutrient cycle mainly to balance the lost of nitrogen in coffee bean harvesting.Key Words: Coffea canephora, Michelia champaca, Gliricidia sepium, Erythrina indica, litter production, nutrient cycle, coffee yield.
Co-Authors Diani Mentari Donny Widianto Irfan D. Prijambada Nastiti Wijayanti Rusdi Evizal Tohari Tohari Tri Wibawa