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Hasnah Natsir
Hasanuddin University
Chemical Engineering, Chemistry & Bioengineering

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STUDY OF COMPOUNDS FROM EXTRACT OF Melochia umbellata (Houtt.) Stapf var. Degrabrata K. (PALIASA) LEAVES THAT HAS POTENTIAL AS ANTIBACTERIAL Selfi Wullur; Firdaus Firdaus; Hasnah Natsir; Nunuk Hariani Soekamto
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 8 No 1 - June 2015
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v8i1.2472

Abstract

Determination of antibacterial activity of each extract of M. umbellata (Houtt.) Stapf var. K. degrabrata leaves has been conducted against three strains bacteria Escherichia coli, Staphylococcus aureus, and Shigella dysenteriae by using amoxicillin as a positive control and DMSO as a negative control. Agar diffusion method has been used with MHA medium (Muller Hinton Agar) on variation concentrations of each extract were 2500 ppm, 5000 ppm, 10,000 ppm, and 20,000 ppm N-hexane extract was the most active extract against S. aureus with zone of inhibitions were 10.50 mm, 10.80 mm, 11.00 mm and 11.45 mm, respectively. The most active extract against S. dysenteriae was ethyl acetate extracts with zone of inhibitions were 9.36 mm, 11.55 mm, 11.58 mm and 17.70 mm, respectively. Isolated compound from n-hexane extract was first compound as white powder with a melting point of 75-77 °C which was identified as 1-Tetracosanol and second compound was obtained in the form of a yellow paste which was identified as steroid compound that had long conjugated substituent. These compounds were elucidated based on the data of IR, UV-Vis, 1H-NMR, and 13C-NMR.
Production and Characterization of Collagenase From Bacillus sp. 6-2 Isolated From Fish Liquid Waste Sartika Danial; Hasnah Natsir; Seniwati Dali; Leliani Leliani
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 12, No.1: June 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v12i1.5924

Abstract

Collagenases are enzyme that are able to hydrolyze native collagen into fragment collagen peptides. Collagenases and its hydrolysis products have received tremendous attention in medical and industrial applications. The present study was conducted to isolate and identify new collagenase producing bacteria from fish liquid waste, then produce and characterize collagenase. A total of 7 isolate from fish liquid waste were screened on selective medium containg 2 % collagen and its activity was confirmed by the formation of clear zone. Isolat 6-2 was positif as collagenase producer and identified as Bacillus sp. 6-2 by morphological and biochemical characteristics. The optimum fermentation time of enzyme was investigated. Collagenase crude extract was characterized by the effect of pH, temperature, and metal ions. Isolat 62 optimally produced collagenase enzyme after 30 h of incubation with activity of   0.072 U/mL and protein content of 3.768 mg/mL. The optimum pH and temperature were 7.0 and 40 oC, respectively. The enzyme was activated by 1 mM Ca2+and  Mg2+, and inhibited by   1 mM  Zn2+ and Co2+. Collagenase from Bacillus sp. 6-2 may have potentials for medical and industrial applications.
Collagen Extraction from Bone of Lutjanus sp. and Toxicity Assay Leliani Ramli; Hasnah Natsir; Seniwati Dali; Sartika Danial
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 12, No.1: June 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v12i1.5925

Abstract

Indonesia is a maritime country with the potential fisheries resources. However, utilization only revolves around the meat, while other parts have not been used optimally, especially fish bones which have the potential to produce collagen, so it needs to be developed. The aims of this study ware extracted collagen from bone of Lutjanus sp. and determine its the Anticancer activity. The collagen was extracted by using hydroextraction method and identification by FTIR. The initial screening anticancer activity was done by using Brine Shrimp Lethality Test (BSLT) method for toxicity assay. The results showed that the yield of collagen was 4.535% with protein concentration was 8,815 mg/mL. Identified collagen from spectrum of amide A, B, I, II, and III at 3421.72; 2926.01; 1651.07; 1541.12; 1240.23 cm-1. The toxicity test was shown in LC50 values of  8,760 μg/mL. The collagen from Lutjanus sp. bone can be used as natural anticancer agent.
Co-Authors Firdaus Firdaus Leliani Leliani Leliani Ramli Nunuk Hariani Soekamto Sartika Danial Selfi Wullur Seniwati Dali