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Bikram Nepali
Agriculture and Forestry University, Rampur, Chitwan, Nepal.
Agriculture, Biological Sciences & Forestry Automotive Engineering Energy Environmental Science

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Identification of Pseudomonas fluorescens using different biochemical tests Bikram Nepali; Sabin Bhattarai; Jiban Shrestha
International Journal of Applied Biology Vol. 2 No. 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i2.5260

Abstract

Pseudomonas fluorescens not only enhances the plant growth but also controls the fungal pathogens by production of anti fungal metabolites. The objective of this experiment was to identify P. fluorescens using different biochemical tests. This research was carried out in Plant Pathology Laboratory at Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal. The result of this experiment indicated that P.  fluorescens gave positive result for Catalase test, Gelatin liquefaction, Fluorescent pigment and Oxidase test but negative result for starch hydrolysis test. The colony of P. fluorescens was maximum in maize seed than that of rice seed after two hours of inoculation, whereas higher number of colony was found in rice seed than that of maize seed after twenty four hours of seed inoculation. This findings is useful for identifying colony of P. fluorescens  per seed which is necessary for better seedling growth and effective biological control of pathogens.
Co-Authors Jiban Shrestha Sabin Bhattarai