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ANALISIS LOGAM BERAT DAGING IKAN BANDENG (Chanos chanos Forsk) MENGGUNAKAN METODE SPEKTOFOTOMETRI SERAPAN ATOM (SSA) : ANALYSIS OF HEAVY METALS IN MEAT MILKFISH (Chanos chanos Forsk) USING ATOMIC ABSORPTION SPECTROPHOTOMETRY (AAS) METHOD Diana Sylvia; Dina Pratiwi; Deasyani Fauziyah
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 7 No 2 (2022)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Perairan laut di Desa Tanjung Burung Kabupaten Tangerang memiliki kekayaan sumber daya laut yang sangat penting untuk mendukung perekonomian masyarakat karena sebagian besar pokok pendapatan masyarakatnya sebagai nelayan, salah satu hasil tangkapan nelayan berupa ikan bandeng. Ekosistem ikan bandeng yang terdapat diperairan dapat menyebabkan ikan bandeng tercemar oleh logam berat. Ikan bandeng merupakan sumber protein bermutu tinggi yang sangat dibutuhkan dalam pertumbuhan, pemeliharaan tubuh serta pengembangan daya pikir. Penelitian ini bertujuan untuk mengetahui kadar logam berat (As, Sn, Pb, Cd dan Hg) pada ikan bandeng (Chanos chanos Forsk). Sampel yang dianalisis sebanyak 2 sampel dengan menggunakan metode Spektofotometri Serapan Atom (SSA). Hasil rata-rata pada ikan bandeng (Chanos chanos Forsk) ikan bandeng A logam berat As -5,9432 mg/kg, Sn 0,1235 mg/kg, Pb 1,6605 mg/kg, Cd -0,0861 mg/kg dan Hg -1,4377 mg/kg. Untuk sampel daging ikan bandeng B logam berat As -5,6718 mg/kg, Sn 0,5820 mg/kg, Pb 1,2195 mg/kg, Cd -0,0958 mg/kg dan Hg - 0,4281 mg/kg. Kadar tersebut masih dalam batas maksimum yang diperbolehkan oleh Standar Nasional Indonesia 7387:2009. Logam As, Cd dan Hg pada sampel ikan bandeng didapatkan hasil minus (-) karena kandungan logam masih dibawah batas deteksi alat Spektofotometri Serapan Atom.

AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI ETANOL-AIR UMBI KIMPUL PUTIH (Xanthosoma sagitafolium L.) DENGAN METODE DPPH Diana Sylvia; Afni Putri Anggraeni; Dina Pratiwi
Jurnal Farmamedika (Pharmamedika Journal) Vol 5 No 1 (2020): Jurnal Farmamedika (Pharmamedica Journal)
Publisher : Sekolah Tinggi Teknologi Industri dan Farmasi Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47219/ath.v5i1.101

Umbi kimpul putih (Xanthosoma sagitafolium (L.) Schott. Secara empiris biasa digunakan sebagai bahan obat tradisional. Umbi kimpul putih mengandung senyawa flavonoid dan polifenol yang diyakini dapat berpotensi sebagai antioksidan. Tujuan penelitian ini adalah untuk menentukan kandungan total fenolik dan aktivitas antioksidan ekstrak etanol 96% dan fraksi etanol-air. Ekstraksi umbi kimpul putih dilakukan dengan metode maserasi dengan pelarut etanol 96% dan difraksinasi berdasarkan tingkat kepolaran dengan pelarut air, etil asetat dan n-heksan. Identifikasi senyawa umbi kimpul putih dilakukan dengan metode skrining fitokimia. Penentuan kandungan total fenolik menggunakan metode Folin Ciocalteau dan pengujian aktivitas antioksidan dilakukan dengan metode DPPH dengan vitamin C sebagai pembanding. Hasil penelitian menunjukkan bahwa metabolit sekunder yang terdapat pada umbi kimpul putih adalah alkaloid, flavonoid, saponin, tanin dan steroid. Berdasarkan hasil yang diperoleh ekstrak etanol 96% memiliki kandungan total fenolik sebesar 7,09 mg/g GAE, diikuti fraksi etanol-air sebesar 5,47 mg/g GAE. Berdasarkan hasil perhitungan nilai IC50 ekstrak etanol 96% memiliki aktivitas antioksidan dengan nilai IC50 sebesar 497,75 ppm, diikuti fraksi etanol-air sebesar 402,36 ppm. Dapat disimpulkan bahwa ekstrak etanol 96% dan fraksi etanol-air umbi kimpul putih memiliki tingkat kekuatan antioksidan yang sangat lemah.

IN SILICO MOLECULAR MODELING AND DOCKING STUDIES OF NANO COMPOSITES COMPOUND TO REGULATION, INHIBITION AND TREATMENT LEAF AND STEM WHEAT RUST Israa Mohamed Shamkh; Dina Pratiwi; Mohammed F. Abo El Magd; Ahmed Salih El Faki
Jurnal Farmagazine Vol 8, No 2 (2021): Jurnal Farmagazine
Publisher : STF Muhammadiyah Tangerang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47653/farm.v8i2.546

Puccinia graminis f. sp. tritici (Pgt) and P. triticina (Pt), the causal agents of stem and leaf rust, respectively form new physiological races that significantly reduce growth and yield of wheat cultivars. Therefore, seeking for exploring if there an inhibition effect of the Nano Composites compound on leaf and stem rust to regulation, inhibition and treatment leaf and stem wheat rust objectives to continuously produce new wheat pesticides resistant to stem and leaf rust. The aim of the study was to finding natural and Nano compounds to control, treatment and regulation of wheat rust. In this study we used molecular modeling and docking for the two vital proteins in stem and leaf wheat rust MAP kinase 1 [Puccinia triticina] and PGTG Puccinia graminis f. sp. Tritici. In the silico analysis, the two vital proteins activity is suppressed and inhibited In this work the chitosan and chitosan –Cu which selected for the study are considered as safe compounds the compounds showed interaction with the MAPK1 and PGAT proteins Thus the bioactive compounds that are interacting with the target can be used as a potent inhibitor to block the action of our proteins.

Development of SARS-CoV-2 Inhibitors Using Molecular Docking Study with Different Coronavirus Spike Protein and ACE2 Israa Mohamed Shamkh; Dina Pratiwi
Journal of Molecular Docking Vol 1 No 1 (2021): Journal of Molecular Docking
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

The novel coronavirus SARS-CoV-2 is an acute respiratory tract infection that emerged in Wuhan city, China. The spike protein of coronaviruses is the main driving force for host cell recognition and is responsible for binding to the ACE2 receptor on the host cell and mediates the fusion of host and viral membranes. Recognizing compounds that could form a complex with the spike protein (S-protein) potently could inhibit SARS-CoV-2 infections. The software was used to survey 300 plant natural compounds or derivatives for their binding ability with the SARS-CoV-2 S-protein. The docking score for ligands towards each protein was calculated to estimate the binding free energy. Four compounds showed a strong ability to bind with the S-protein (neohesperidin, quercetin 3-O-rutinoside-7-O-glucoside, 14-ketostypodiol diacetate, and hydroxypropyl methylcellulose) and used to predict its docking model and binding regions. The highest predicted ligand/protein affinity was with quercetin 3-O-rutinoside-7-O-glucoside followed by neohesperidin. The four compounds were also tested against other related coronavirus and showed their binding ability to S-protein of the bat, SARS, and MERS coronavirus strains, indicating that they could bind and block the spike activities and subsequently prevent them infection of different coronaviruses. Molecular docking also showed the probability of the four ligands binding to the host cell receptor ACE2. The interaction residues and the binding energy for the complexes were identified. The strong binding ability of the four compounds to the S-protein and the ACE2 protein indicates that they might be used to develop therapeutics specific against SARS-CoV-2 and close related human coronaviruses.

In Silico Study for Similar FDA Approved Drugs as Inhibitors of SARS-CoV-2 Spike and the Host Receptor Proteins Israa Mohamed Shamkh; Dina Pratiwi; Hanaa S. Omar; Nour El-Houda A. Reyad
Journal of Molecular Docking Vol 1 No 2 (2021): Journal of Molecular Docking
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

The severe acute respiratory syndrome coronavirus 2, known as COVID-19, has been hideously increased worldwide. The disease began in Wuhan, China, around December 2019, then spread to most countries. Social distancing is the best procedure to prevent infection. Screening the available database containing millions of drug molecules or phytochemicals has become rapid and straightforward because of the computer-aided drug design (CADD) methods. In the present study, 300 phytochemicals and cellulose ether derivatives are screened through a docking study. Docking analysis showed that only four molecules (a-neohesperidin, quercetin 3-O-glucosylrutinoside, 14-ketostypodiol diacetate, and hydroxypropyl methylcellulose) were able to interact with the spike protein. However, two among them (quercetin 3-O-glucosylrutinoside and 14-ketostypodiol diacetate) could interact with the host cell receptor (ACE2) of SARS-CoV-2. The binding affinity of the four compounds is high. Still, according to Lipinski's rule of five, only 14-ketostypodiol diacetate was selected as a drug molecule due to its pharmacokinetic and ADMET properties. Screening for drug analogs to the 14-ketostypodiol diacetate detected five approved drugs. Docking analysis of these drugs with the target proteins showed that the five drugs interact with the host receptor protein, and three interact with viral spike protein. Accordingly, we suggest that molecular docking and drug analogs studies could support rapid drug development. In addition, future perspectives on therapeutic applications of 14-ketostypodiol diacetate are required for using it against SARS-CoV-2 infections.

Corrigendum to "Development of SARS-CoV-2 Inhibitors Using Molecular Docking Study with Different Coronavirus Spike Protein and ACE2” [J Mol Docking. 2021;1(2):1-14] Israa Mohamed Shamkh; Dina Pratiwi; Hanaa S. Omar
Journal of Molecular Docking Vol 1 No 2 (2021): Journal of Molecular Docking
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Authors have found an error in the previous version (Shamkh, IM, & Pratiwi, D. (2021). Development of SARS-CoV-2 Inhibitors Using Molecular Docking Study with Different Coronavirus Spike Protein and ACE2. Journal of Molecular Docking, 1(1), 1-14. https://doi.org/10.33084/jmd.v1i1.2212), of which Dr. Hanaa S. Omar as supervisor of the research, is not listed as one of the authors. In this note, Dr. Hanaa S. Omar was added as one of the authors, with the status of the corresponding author in the study.

PREDIKSI TOKSISITAS DAN MOLECULAR DOCKING SENYAWA ANTIOKSIDAN ALAMIGOLONGAN STEROID YANG BERASAL DARI TUMBUHAN PADA RESEPTOR ANDROGEN, ESTROGEN, DAN PROGESTERON Dina Pratiwi
INDONESIA NATURAL RESEARCH PHARMACEUTICAL JOURNAL Vol 1, No 1 (2016)
Publisher : Fakultas Farmasi Universitas 17 Agustus 1945 Jakarta

ABSTRAK Penelitian ini bertujuan untuk memperoleh prediksi toksisitas senyawa antioksidan alami menggunakan metode in silico dan analisis interaksinya dengan reseptor melalui metode molecular docking. Penelitian ini menggunakanenam senyawa antioksidan alami golongan steroid yang berasal dari tumbuhan yaitu D5-avenasterol, D7-avenasterol, β-sitosterol, brasikasterol, kampesterol, dan stigmasterol. Semua senyawa diprediksi toksisitasnya menggunakan perangkat lunak ADMET predictor, Toxtree, dan QSAR Toolbox,sedangkan proses dockingdilakukan menggunakan perangkat lunak Autodock 4.2.6. Hasil prediksi toksisitas menunjukkan bahwa enam senyawa tersebut diprediksi toksik terhadap sistem reproduksi. Hasil analisis dockingterhadap reseptorandrogen (2AM9), estrogen (1A52), dan progesteron (1A28) yang diperoleh dari Protein Data Bank (PDB) menunjukkan bahwa senyawa D7-avenasterol dan β-sitosterol memiliki energi bebas ikatan yang lebih rendah dibandingkan dengan estradiol dan progesteron sebagai senyawa pembanding pada reseptor estrogen alfa dan progesteron. Kata Kunci: molecular docking, prediksi toksisitas, steroid tumbuhan

ANALISIS LOGAM BERAT DAGING IKAN BANDENG (Chanos chanos Forsk) MENGGUNAKAN METODE SPEKTOFOTOMETRI SERAPAN ATOM (SSA) : ANALYSIS OF HEAVY METALS IN MEAT MILKFISH (Chanos chanos Forsk) USING ATOMIC ABSORPTION SPECTROPHOTOMETRY (AAS) METHOD Diana Sylvia; Dina Pratiwi; Deasyani Fauziyah
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 7 No 2 (2022)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v7i2.331

Perairan laut di Desa Tanjung Burung Kabupaten Tangerang memiliki kekayaan sumber daya laut yang sangat penting untuk mendukung perekonomian masyarakat karena sebagian besar pokok pendapatan masyarakatnya sebagai nelayan, salah satu hasil tangkapan nelayan berupa ikan bandeng. Ekosistem ikan bandeng yang terdapat diperairan dapat menyebabkan ikan bandeng tercemar oleh logam berat. Ikan bandeng merupakan sumber protein bermutu tinggi yang sangat dibutuhkan dalam pertumbuhan, pemeliharaan tubuh serta pengembangan daya pikir. Penelitian ini bertujuan untuk mengetahui kadar logam berat (As, Sn, Pb, Cd dan Hg) pada ikan bandeng (Chanos chanos Forsk). Sampel yang dianalisis sebanyak 2 sampel dengan menggunakan metode Spektofotometri Serapan Atom (SSA). Hasil rata-rata pada ikan bandeng (Chanos chanos Forsk) ikan bandeng A logam berat As -5,9432 mg/kg, Sn 0,1235 mg/kg, Pb 1,6605 mg/kg, Cd -0,0861 mg/kg dan Hg -1,4377 mg/kg. Untuk sampel daging ikan bandeng B logam berat As -5,6718 mg/kg, Sn 0,5820 mg/kg, Pb 1,2195 mg/kg, Cd -0,0958 mg/kg dan Hg - 0,4281 mg/kg. Kadar tersebut masih dalam batas maksimum yang diperbolehkan oleh Standar Nasional Indonesia 7387:2009. Logam As, Cd dan Hg pada sampel ikan bandeng didapatkan hasil minus (-) karena kandungan logam masih dibawah batas deteksi alat Spektofotometri Serapan Atom.

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