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All Journal Jurnal Kedokteran Diponegoro Journal of Biomedicine and Translational Research
Kis Djamiatun
Faculty of Medicine Diponegoro University
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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The Effect of Eurycoma longifolia Jack on sICAM-1 and eNOS in Rats with High Fat Diet Anas Omar Ashkurfu; Kis Djamiatun
Journal of Biomedicine and Translational Research Vol 4, No 1 (2018): July 2018
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (782.904 KB) | DOI: 10.14710/jbtr.v4i1.2670

Abstract

Background: High fat diets are known to cause a positive fat balance and consequently to the accumulation of adipose mass, this diet does not seem to stimulate fat oxidation in the same way in obese and lean subjects. HFD was an inducing factor for ICAM-1 expression in the aorta of Wistar rats. HFD effect on ICAM-1 seems to be time dependent. ICAM-1 is one of the first events in the formation of atherosclerotic lesions. HFD up-regulated Cav-1, regulated expression other biomarker in HFD is eNOS. Recent studies showed that E. longifolia Jack protected HFD animal model from atherosclerosis based on the reduce atherosclerotic plaque size and formation HFD-rats treated with E. longifolia Jack.Objective: To prove that Eurycoma longifolia has anti inflammatory effect on endothelial cell blood vessels of Sprague Dawley rat with high fat diet.Method: Study design was experimental study, by used Randomized Post Test only Control Group Design with Kruskal-Wallis test was used to analyze the differences among groups and followed by a Mann Whitney test. Treatment is ethanolic or water extract of Eurycoma longifolia Jack, and out come are sICAM-1 and eNOS levels. Thirty Sprague Dawley (SD) Rat, were divided into 6 groups. C(-) was SD group, C(+) was group with HFD, X1 (SD treated with EL dosage 10 mg/kg), X2 (SD treated with EL dosage 15 mg/kg), X3 (HFD treated with EL dosage 10 mg/kg), X4 (HFD treated with EL dosage 15 mg/kg).
The Effect of Extra Virgin Olive Oil on LOX-1 and COX-2 in High Fat Diet Rats Ibrahem Omran Lakhder; Lisyani Suromo; Kis Djamiatun
Journal of Biomedicine and Translational Research Vol 2, No 1 (2016): July 2016
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.949 KB) | DOI: 10.14710/jbtr.v2i1.331

Abstract

Background: High fat diet is a diet containing large amounts of fat consistently, the increase dietary fat and cholesterol which have a key role in growing health problems. Extra virgin olive oil associated with prevention of LDL oxidation, beneficial changes in lipid ratios and low risk for CHD.Objective: to determine the anti-inflammatory effect  of extra virgin olive oil extract to  levels of COX-2 and LOX-1 in the blood in rats induced by high fat dietMethods: This research is an experimental study that used randomized posttest only control group design. 30 Wistar rats which were divided into five groups:  group of control (-) which received  normal diet and group of control (+) which received  high fat diet without  EVOO treatment and three high fat diet groups treated by EVOO 1 mL/kg/day, 2 mL/kg/day and 3 mL/kg/day orally for 2 months. The blood was collected from eyes rats and serum separation by centrifuge. COX-2, LOX-1 concentration was measured by the enzyme linked immunosorbent assay (ELISA).Results: The result of this study showed higher COX-2 concentrations in groups treated with EVOO than control group. COX-2 serum levels of negative control where significantly lower than those of rats treated with 2 ml/kg/day (p = 0.047) and 3 ml/kg/day EVOO (p = 0.014). The COX-2 serum levels of group received 1 ml/kg/day were significantly lower than those of rats received 3 ml/kg/day EVOO (p = 0.027). And showed  not significantly deferent among all groups (p = 0.570).Conclusions: The conclusion of this study has showed that extra virgin olive oil extract might have minor anti inflammatory and antioxidant effect in rats.
SONGGA WOOD (STRYCHNOS LIGUSTRINA BLUME) ASSOCIATED WITH REDUCE TUMOR NECROSIS FACTOR-ALPHA LEVELS IN ARTEMINISIN-BASED-COMBINATION THERAPY-TREATED MALARIA Kis Djamiatun
Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) Vol 14, No 4 (2025): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkd (dmj).v14i4.49555

Abstract

Background: The emergence of resistance to artemisinin-based combination therapies (ACTs) complicates malaria control strategies, necessitating novel therapeutic approaches and enhanced surveillance in affected regions. Traditional herbal remedies, such as Songga wood (Strychnos ligustrina Blume), are gaining attention for their potential antimalarial and immunomodulatory properties, offering promising alternatives to combat drug-resistant malaria. Objective: This study aimed to evaluate the effect of ethanolic extracts from the stem of Songga wood (EESWS) on the modulation of TNF-α in malaria treatment with ACTs. Methods: A study used a post-test-only-control-group design with simple-random-sampling involved two treatment groups and three control groups, each with five Swiss-Webster mice. The C1 group was the healthy control, the C2 group was untreated, and the C3 group received ACT. The P1 group was given EESWS at preventive and therapeutic doses, while the P2 group received a combination of EESWS-ACT. Blood samples were collected on the day of the 8th infection to assess parasitemia percentage and plasma TNF-α levels. Results: On day 7 of PbA infection, the untreated C2 group exhibited the highest parasitemia levels (13.20 ± 4.18), while the C3, P1, and P2 groups showed significantly lower levels, with no significant differences among these treated groups (p > 0.05). Significant differences in TNF-α levels were observed, with the untreated C2 group having higher levels compared to the healthy C1 group and all treatment groups. Among the treatments, the P1 group had higher TNF-α levels than both the P2 and C3 groups, while no significant difference was observed between the C3 and P2 groups. Conclusions: All treatment regimens effectively promoted recovery from PbA infection, and the combination of EESWS with ACT appears to facilitate a more balanced modulation of inflammatory responses during the malaria recovery phase.
Co-Authors Anas Omar Ashkurfu Ibrahem Omran Lakhder Lisyani Suromo