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Struktur Populasi dan Nilai Sintasan Alami Semai Cembirit (Tabernaemontana macrocarpa) Kohort Daun Lebih dari 12 di Hutan Lindung Mangunan Alfino Sebastian
Quagga : Jurnal Pendidikan dan Biologi Vol 14, No 1 (2022): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v14i1.4832

Abstract

Salah satu hutan lindung yang terletak di Yogyakarta adalah hutan lindung Mangunan, yang terdapat tegakan dominan yaitu Akasia, tetapi akhir- akhir ini terdapat tegakan yang mengancam akan menggeser tegakan Akasia yaitu Cembirit. Hal ini dapat dilihat dari meningkatnya densitas seedling dan sapling cembirit dari tahun 2016 sebesar (520 ind/Ha dan 26.800 ind/Ha) menjadi 860 ind/Ha dan 30.200 ind/Ha pada tahun 2017. Oleh karena itu penelitian ini dilakukan untuk mengetahui Struktur populasi dan kompetisi intraspesifik pada seedling cembirit (T. macrocarpa) di Hutan Lindung Mangunan, Dlingo, Yogyakarta guna mengetahui nilai sintasan di masing – masing kohortnya agar dapat dijadikan sebagai acuan dalam pengendalian populasinya. Dibawah lima pohon cembirit yang seragam dan produktif dibuat 2 Plot dengan ukuran 1 x 0,5m, sebagai kontrol dan perlakuan. Dalam perlakuan dilakukan dengan mencabut semua tanaman cembirit yang ada kecuali pada kohort daun lebih dari 12. Selanjutnya dilakukan pencatatan seedling cembirit yang terdapat didalam kedua plot tersebut, setiap 2 minggu sekali selama 6 kali pengambilan data. Diperoleh hasil bahwa jumlah individu terbanyak adalah pada semai cembirit kohort daun 2 – 4 dan paling rendah pada kohort daun lebih dari 12 sebesar 5 individu. Semai Cembirit kohort daun lebih dari 12 memiliki nilai sintasan rata – rata pada kontrol sebesar 1.32 dan perlakuan adalah 1.02.  One of Hutan Lindung that located in Yogyakarta is Hutan Lindung Mangunan, which has dominant stands of Acacia, but recently there are stands that threat the Acacia stands ie Cembirit. This was indicated from the increase of seedling  and sapling densities of cembirit from (520 ind / Ha and 26,800 ind / Ha) in 2016 to 860 ind / Ha and 30,200 ind / Ha in 2017. Therefore this research is conducted to know the population structure and intraspecific competition on seedling cembirit (T. macrocarpa) in Hutan Lindung Mangunan, Dlingo, Yogyakarta to find out the value of synthesis in each cohort so that it can be used as a reference in the control of the population. The method is by choosing five Cembirit trees with a uniform basal area and still productive. Under the five trees are made 2 plots of 1 x 0.5 m, as control and treatment. The treatment is done by removing all existing cembirit plants except population with the leaf cohort more than 12. Furthermore, the recording of cembirit seedling in both plots were carried out, every 2 weeks for 6 times data retrieval. The results showed that in leaf cembirit  2 - 4 there are 184 individuals, on leaves 6 to 8 a total of 127 individuals, on leaves 10-12 showed the number of 18 individuals, and in the leaf cohort more than 12 for 5 individuals. with a survivorship curve in plants. Cembirit leaf Seedling cohorts more than 12 had an average survival rate value on the control of 1.32 and the treatment was 1.02.
Optimization of heat shock temperature and time on the transformation of pRGEB32 into Escherichia coli DH5α Rosy Feraningsih Patigu; Putri Wijayanti; Alfino Sebastian; Yekti Asih Purwestri
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.2811

Abstract

Genome editing technique is one of the methods for studying the expression of gene, eliminating unfavorable traits or phenotypes and generating the new characters of species. The pRGEB32 plasmid is one of the vectors that used in genome editing with carrying the Cas9 gene, restriction site of sgRNA (single guide RNA) and specific promoters that can be expressed in plants. The first step in the genome editing process is inserting pRGEB32 into Escherichia coli for propagation. The large size of the plasmid molecule becomes a challenge to determine the right method in the transformation process. This study aims to determine the temperature and time of heat shock transformation of plasmid pRGEB32 into E. coli. The transformation of pRGEB32 into plasmids was carried out with variations in temperature and time, 42℃ (30 seconds and 60 seconds) and 55℃ (30 seconds and 60 seconds). The results showed that a heat shock temperature of 55℃ with a time of 60 seconds was the best temperature for the transformation of pRGEB32 into E. coli. This optimization of heat shock condition will increase the transformation efficiency, which is in the range of 3322-10.989 cfu/µg.ÂÂ