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All Journal The Journal of Experimental Life Sciences (JELS) Research Journal of Life Science
Arning Wilujeng Ekawati
University of Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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Journal : Research Journal of Life Science

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Potential the Black Soldier Fly (Hermetia illucens) in Feed Formulation for Growth of Common Carp (Cyprinus carpio L.) Ayu Azkiyah Azizah; Arning Wilujeng Ekawati; Happy Nursyam
Research Journal of Life Science Vol 7, No 3 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.03.5

Abstract

The utilization of maggot as larvae from black soldier fly is an alternative substitution of fish meal as the primary protein source in fish feed. Maggot is cultivated in 3 different cultivation, the medium used is organic waste, tofu waste, and wheat pollard. The method that used in this research is Completely Randomized Design (CRD) with 3 treatments and 3 replication. This study aims to determine the effect of various types of maggot growth media, 1) with maggot biomass parameters, containers, and growing media conditions maggot, 2) then, exact proximate composition, total amino acids content of the prepupae samples were determined. Based on the results of the analysis, each dose of treatment (media) has an influence on the different maggot biomass. The best media treatment results are pollard flour. The proximate analysis showed that BSF larvae had a protein content of 50,88% and also fat content of 15%.
Active Compounds on Squid (Loligo sp.) Ink Extract Powder as Immunostimulant Candidate to Against Shrimp Disease Rangga Idris Affandi; Mohamad Fadjar; Arning Wilujeng Ekawati
Research Journal of Life Science Vol 6, No 3 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.685 KB) | DOI: 10.21776/ub.rjls.2019.006.03.1

Abstract

The business of developing shrimp farming cannot be separated from the existence of disease. In shrimp farming health management, disease prevention strategies can using various methods, one of which is immunostimulant. One alternative immunostimulant source that can be used to improve the body defense system in shrimp is squid (Loligo sp.) ink extract powder who has antibacterial ability. This study aims to analyze the presence of active compound in squid ink extract powder can be used as an immunostimulant candidate to against shrimp disease. The method used in this research is descriptive explorative and experimental method. This research was conducted with several stages of squid ink extraction until it becomes powder. Identification of squid ink extract powder is using FTIR and LC-MS test. The results showed that the squid ink extract powder contained alkaloid and carboxylic acid from the FTIR test results. Based on LC-MS test results, it was found that squid ink extract powder contained betaine, cinnamic acid, and choline compounds with large amounts of content. Betaine, cinnamic acid, and choline has several biological activity as antibacterial, antioxidant, antiviral, antifungal, etc. so that it can be used as an immunostimulant against shrimp disease.
Characteristics of Tuna Viscera (Thunnus sp.) Hydrolysate Protein Fermented by Bacillus licheniformis Galih Nugroho; Arning Wilujeng Ekawati; Hartati Kartikaningsih
Research Journal of Life Science Vol 7, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2020.007.02.4

Abstract

This study aims to analyze the nutritional composition of the degree of hydrolysis and amino acids in the internal organ waste of tuna and the protein hydrolyzate of tuna's internal organs after fermentation using the Bacillus licheniformis bacteria. The analysis showed that the protein content of tuna offal was 53.52%, and after fermentation by Bacillus licheniformis, bacteria were able to increase protein levels from 56.04. The degree of hydrolysis of protein (DH) showed an increase of 13.24% in tuna offal to 22.28% of protein hydrolyzates are fermented tuna innards. The total essential amino acids and non-essential amino acids in the fermented tuna innards' protein hydrolyzates increased during the fermentation process. The highest levels of essential amino acids were found in arginine as high as 3.632965 at the 96th hour, and the lowest histidine was 1.082602. In contrast, for the highest non-essential amino acids, there was glycine at 8.52223, and the lowest for tyrosine was 1.272592.
Co-Authors Ayu Azkiyah Azizah Galih Nugroho Happy Nursyam Kartikaningsih, Hartati Mohamad Fadjar Muhammad Ikhwan Ihtifazhuddin Rangga Idris Affandi