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All Journal Research Journal of Life Science Jurnal Penelitian Pendidikan IPA (JPPIPA)
Mohamad Fadjar
University of Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Medicine & Pharmacology Physics

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Active Compounds on Squid (Loligo sp.) Ink Extract Powder as Immunostimulant Candidate to Against Shrimp Disease Rangga Idris Affandi; Mohamad Fadjar; Arning Wilujeng Ekawati
Research Journal of Life Science Vol 6, No 3 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.685 KB) | DOI: 10.21776/ub.rjls.2019.006.03.1

Abstract

The business of developing shrimp farming cannot be separated from the existence of disease. In shrimp farming health management, disease prevention strategies can using various methods, one of which is immunostimulant. One alternative immunostimulant source that can be used to improve the body defense system in shrimp is squid (Loligo sp.) ink extract powder who has antibacterial ability. This study aims to analyze the presence of active compound in squid ink extract powder can be used as an immunostimulant candidate to against shrimp disease. The method used in this research is descriptive explorative and experimental method. This research was conducted with several stages of squid ink extraction until it becomes powder. Identification of squid ink extract powder is using FTIR and LC-MS test. The results showed that the squid ink extract powder contained alkaloid and carboxylic acid from the FTIR test results. Based on LC-MS test results, it was found that squid ink extract powder contained betaine, cinnamic acid, and choline compounds with large amounts of content. Betaine, cinnamic acid, and choline has several biological activity as antibacterial, antioxidant, antiviral, antifungal, etc. so that it can be used as an immunostimulant against shrimp disease.
Pengaruh Ekstrak Kasar Daun Keji Beling (Strobilanthes crispus) terhadap Infeksi Bakteri Aeromonas hydrophila Secara In Vitro Genesia Gerrine; Arief Prajitno; Mohamad Fadjar; Rahmalia Eka Kenitasari
Jurnal Penelitian Pendidikan IPA Vol 9 No 5 (2023): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i5.3502

Abstract

Keji beling leaves (Strobilanthes crispus) can be the solution to cure the fish that infected by Aeromonas hydrophila because it contained active compounds. The purpose of this study was to determine the active compounds contained in the crude extract of keji beling leaves (S. crispus) and their ability to inhibit the growth of A. hydrophila. The tests which are used in this study were phytochemical test, MIC test, disk diffusion test, and SEM test. Phytochemicals analysis showed the presence of active compounds are flavonoid, alkaloid, tanin, and triterpenoid. The result of MIC test showed that concentration of 100 mg/l with a value of 0.389 was the lowest absorbance of the keji beling (S. crispus) crude leaves extract. The inhibition test used disk diffusin test with five concentrations of keji beling (S. crispus) crude leaves extract with three replications: 50 mg/l, 100 mg/l, 150 mg/l, 200 mg/l, and 250 mg/l, with the observation time of 24 hours post-incubation and 48 hours post-incubation. The concentration of 250 mg/l had the highest inhibition zone. The result of SEM analysis showed that keji beling (S. crispus) crude leaves extract can damage the cell walls of A. hydrophila and lysis the bacterial cells.
Co-Authors Arief Prajitno Arning Wilujeng Ekawati Genesia Gerrine Rahmalia Eka Kenitasari Rangga Idris Affandi