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Nandang Suharna
Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Cibinong, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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INTERAKSI ANTARA Trichoderma harzianum, Penicillium sp. DAN Pseudomonas sp. SERTA KAPASITAS ANTAGONISMENYA TERHADAP Phytophthora capsicilN VITRO Nandang Suharna
BERITA BIOLOGI Vol 6, No 6 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i6.1203

Abstract

A preliminary study has been done to know antagonism capacities of three isolates of Trichoderma harzianum, two isolates of Penicillium sp.and one isolate of Pseudomonas sp.against Phytophthora capsici in vitro and interaction among those six antagonists.The highest antagonism capacity possessed by Penicillium sp. KN1, respectively followed by Penicillium sp.KN2,Pseudomonas sp. GH1 and the three T. harzianum isolates. Except for those three T. harzianum isolates, the two Penicillium sp.isolates and Pseudomonas sp. GH1 isolate indicated anti fungal activity against this fungal pathogen.Based on microscopic observation, there was no mycoparasitism within three T.harzianum isolates against Ph. capsici.While interaction occurred among antagonist showed that Pseudomonas sp.GH1 was antagonistic against the other five antagonists.Growth inhibition by Penicillium sp. KN2 showed against this plant pathogen.Beside the need of further study in green house and field, this result appears the need of study to clarify and identify of the chemical subtance of anti fungal possessed by Penicillium sp. KN1 dan Penicillium sp. KN2.The result showed that the six microbes most potential for biological control agents against Ph. capsici.
STUDI AWAL KEBERADAAN JAMUR TANAH PERAKARAN TUMBUHAN DI KAWASAN STASIUN PENELITIAN KETAMBE, TAMAN NASIONAL GUNUNG LEUSER, ACEH TENGGARA Nandang Suharna
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1274

Abstract

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RHIZOSPHERE FUNGI OF GUNUNG HALIMUN NATIONAL PARK Nandang Suharna
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1079

Abstract

An ecological study was conducted to assess the occurrences of rhizosphere fungi in three dominating trees (Altingia excelsa, Schima wallichii and Castanopsis javanica) within Gunung Halimun National Park.Rhizosphere trees in Gunung Halimun National Park was mainly dominated by Trichoderma (47%), followed by Penicillium (16%), Cunninghamella (10%),Mortierella (15%),Acremonium (7%), and Humicola (6%).Similar composition of rhizosphere fungi was shown by the three trees and mainly consisted of Trichoderma and Penicillium.There was negative correlation of rhizosphere to fungal composition which was shown by C.javanicus and the positive correlation was shown by A. excelsa and S. wallichii.Based on total of fungi encountered, Trichoderma was found to be the most common and dominant fungus in rhizosphere of those trees.Other fungi were also considered common were Penicillium, Mortierella, Cunninghamella, Acremonium and Humicola. Based on the number of samples, the frequency of the occurrence of Trichoderma was the highest (100%), followed by Penicillium (88%),Cunninghamella (59%), Humicola (47%), Acremonium (41%), Mortierella (35%), Verticillium (18%), Cylindrocladium (12%), Aspergillus (6%), and Gliocephalotrichum (6%).
EFEKTIVITAS ALKOHOL DAN CAMPURANNYA DENGAN FORMALIN SEBAGAI BAHAN PENGAWET SPESIMEN BINATANG UNTUK UDANG Nandang Suharna; Rita Dwi Rahayu
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1099

Abstract

This study was aimed at studying the effectiveness of alcohol, formaldehyde and their mixture in inhibiting fungal growth.This study contained two step. The first step was fungal isolation from specimen.One isolate was then selected to be inoculated into alcoholat various concentration (15%, 30%, 50% and 70%) with or without formaldehyde mixture.The second was fungal inoculation intoalcohol at various concentration (15%, 30%, 50%, dan 70%) with or without formaldehyde.The effectiveness of these subtances as specimen preservatives for shrimp were studied after six months incubation time.From the fisrt step, only genus group Monascus was isolated from seven specimens(Mabuia multifasciata, Myotis muricola,Chironax melanocephalus,Naya naya sputratrix, Miniopterus schreibersi blepotis,unidentified shrimp, unidentified Coelenterata).From the second step, the best growth of isolate Monascus sp.MM which isolated from the fisrt step was achieved in 70% alcohol.This fungus was not able to grow in both formaldehyde and its mixture with alcohol (1:1, 1:2, 1:3 and 1:4). The results also showed that Monascus growth was observed in alcohol with not sterilized shrimps without inoculation with Monascus sp. MM. It is recommended to apply mixture alcohol 70% with formaldehyde 1% at ratio 4:1 as animal specimen preservative to inhibit fungal growth.
ANALISIS DELIMITASI JENIS PADA Monascus Spp. MENGGUNAKAN SIDIK JARI DNA ARBITRARY PRIMER PCR Nandang Suharna; Heddy Julistiono
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2928

Abstract

A species delimitation analysis within Monascus spp. using Arbitrary Primer Polymerase Chain Reaction (AP PCR) DNA fingerprint was carried out. This is one of the methods used for identification and discrimination of bacterial strains within the same species. Its advantages including using single primer, independent of DNA quality, and observing amplicon shared by only some strain. This study analyzed Monascus sp. MM isolate which was originated from a source contaning high level of ethanol and two M. purpureus isolates which isolated from angkak. However, based on ITS region, 99% homology showed the unclear species delimitation. Therefore, this analysis was aimed at clarifying on the identities of Monascus species tested. The result showed DNA polymorphism among three isolates of Monascus that showed species delimitation. This study showed that species delimitation within Monascus isolates used in this analysis could be supported by AP PCR DNA fingerprinting. Therefor we suggested to use this technique or method for phylogenetic study to clarify taxonomic position of Monascus strains. 
KEBERADAAN DAN DISTRIBUSl JENIS-JENIS Trichoderma DI HUTAN KAWASAN TAMAN NASIONAL GUNUNG HALIMUN Nandang Suharna
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1184

Abstract

A study was conducted m determine the occurrence and distribution of Trichoderma species in forest in Gunung Halimun National Park. Location for data ollection were Cikaniki (1.100 asl), Citalahab (1.100 asl.)and Gunung Botol (1.500 asl, 1.600 asl,and 1.700 asl).Isolation of Trichoderma were from non vegetation soils, rhizosphere soils and stumps of three dominating forest trees i.e.Allingia excelsa,Caslanopsis javanicti and Schimu wallichii.The isolation method used were soil plate (non vegetation soil,Rhizosphere soil), dilution plate (non vegetation soil.Rhizosphere soil) and surface sterilization (stump).Trichoderma viride recorded as the commonest fungus of its occurrence and the most widespread in non vegetation soil in all location studied. Other Trichoderma species encountered were T. longibrachialuin, T. virens T. koningii.T. hamaium. T. pseudokoningii and T. polysporum. While in rhizosphere soils of the three dominating trees {Allingia excelsa. Schima wallichii. and Casianopsis javanica) located in Cikaniki, T. hamatum, was the commonest fungus of its occurrence. Another common was T. koningii. Other species were rarely encountered were T. longibrachialum, T. virens, T. pseudokoningii and T. polysporum, and T. fertile. In stumps of four trees in Cikaniki four species of Trichoderma were recorded of their occurrences. They were T. harzianum (Memecylon excelsum). T. koningii (S. wallichii), T. viride (A. excelsa, S. wallichii and C. javanica) and T. virens (A. excelsa and M. excelsum). It i> concluded that in forest of Gunung Halimun National Park, habitat influenced on the occurrence and distribution of Trichoderma species, but not for altitude. Trichoderma was high in diversity, dominant in occurrence and widespread in distribution.
Co-Authors Heddy Julistiono Rita Dwi Rahayu