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All Journal AGRIVITA, Journal of Agricultural Science Agrotechnology Research Journal
Supyani Supyani
Universitas Sebelas Maret
Agriculture, Biological Sciences & Forestry

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HYPOVIRULENT ISOLATES OF FUSARIUM COLLECTED FROM CHILI CROPS IN BOYOLALI REGENCY, CENTRAL JAVA, INDONESIA Supyani Supyani; Sri Widadi
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i1.467

Abstract

Fusarium, a genus of filamentous fungi, has many species which serving as important pathogens to many diseases in crops. Till today, there have not been effective and efficient control methods for such fungi. Recently, scientists agree that application of biological agents is a tactful choice. Development of hypovirulent strains of fungus as biocontrol agents is very limited. This research was aimed to find hypovirulent isolates of Fusarium from field as biological agents. A hundred isolates of Fusarium from chili were collected in Boyolali, Central Java. Morphological characterization revealed that isolates performed varied colony phenotypes. Based on colony phenotype pattern, isolates were classified into five groups. From each group, one hypovirulent isolate was selected based on colony growth rate on potato dextrose agar media. The selected hypovirulent isolates were used for virulence assay in apple. The result showed that there were four hypovirulent isolates i.e.: B6, C15, D19, and E20 isolates. Total RNA extraction of the identified hypovirulent isolates revealed the existence of viral RNA in C15 isolate. Based on the existence of viral RNA in C15 isolate, the hypovirulent traits were due to mycoviral infection, whereas the hypovirulent traits performed by the other three were due to genetic factors.
Keberadaan Mikovirus Berdasarkan Deteksi Berbasis RNA pada Colletotrichum Hipovirulen Lulu'il Maknun; Supyani Supyani; Hadiwiyono Hadiwiyono; Budi Tjahjono
Agrotechnology Research Journal Vol 3, No 1 (2019): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.442 KB) | DOI: 10.20961/agrotechresj.v3i1.27596

Abstract

Fertilization is any effort that aims to increase the availability of nutrients needed by plants, and increase crop production. The study aimed to obtain fertilizer types, fertilizer dosages and combinations of types and dosages of fertilizers suitable for growth and yield of Crystal Guava plants. The study was conducted on October 2018 to Mei 2019 on farmer’s land, Banjarsari Kulon Village, Sumbang District, Banyumas Regency and Agronomy and Horticulture Laboratory of the Faculty of Agriculture, Jenderal Sudirman University. The experimental design used was a Randomized Completely Block Design (RCBD) with 2 factors and 3 replications. The first factor was the type of fertilizer, namely NPK, SP-36 and MKP fertilizers. The second factor was fertilizer dosage, namely 20 g / plant, 30 g / plant, 40 g / plant and 50 g / plant. Observed data were done by Analysis of Variance and if it significantly affected then DMRT (Duncan’s Multiple Range Test) was carried out at the level of 5%. The results showed that the most effective type of NPK fertilizer and dosage of 20 g / plant. The combination of NPK fertilizer with a dose of 20 g / plant was the most effective treatment for increasing the growth and yields of the Crystal Guava plant.
Co-Authors Budi Tjahjono Hadiwiyono Hadiwiyono Lulu'il Maknun Sri Widadi