Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal AGRIVITA, Journal of Agricultural Science
Edison HS
Indonesian Tropical Fruit Research Institute, Solok, West Sumatera, Indonesia
Agriculture, Biological Sciences & Forestry

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Genetic Stability of Banana Plant Regenerated from Floral Axis Organogenesis Assessed by Newly Developed SSR Markers Puji Lestari; I. Roostika; Kristianto Nugroho; Edison HS; Habib Rijzaani; Mastur Mastur
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.1931

Abstract

Molecular marker is robust to precisely monitor the genetic stability of in vitro-banana plants. This study examined the genetic stability of 8 monthold banana plants of Soka variety derived from floral axis organogenesis using newly developed SSR markers. The results showed that the same qualitative and similar quantitative morphological characters of pseudostem, leaf and fruit were identified between mother plants and culture plants from floral axis regeneration. Both plants types were quite similar in number of tillers, brix percentage, fruit peel/mesocarp thickness and fruit length. Eleven out of 211 good quality of SSR loci showing high homology with important genes were selected for suitable PCR primers and produced unambiguous bands.The number of total bands was 323 for total SSR primers, in range of 20-60 per primer for total individual plants. Most culture plants showed identical with their mother plants, with very minor variation as reflected by genetic similarity coefficient range of 0.9-1.0. A high similar pattern on SSR to support morphological characters of mother plants and culture plants indicated a successful micropropagation using floral axis to encounter off-type clones.The floral axis organogenesis in this study is able to provide sufficient genetic materials of Soka for varietal registration and other applications.
Co-Authors Habib Rijzaani I. Roostika Kristianto Nugroho Mastur Mastur Puji Lestari