Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Journal of Mathematical and Fundamental Sciences
L. Ambarsari
Biokimia, FMIPA Institut Teknologi Bandung
Astronomy Chemistry Earth & Planetary Sciences Mathematics Physics

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Pengaruh Mutasi D802N pada Aktivitas Polimerase DNA Pol I ITB-1 L. Ambarsari; F. Madayanti; M. R. Moeis; Akhmaloka Akhmaloka
Journal of Mathematical and Fundamental Sciences Vol. 38 No. 2 (2006)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/itbj.sci.2006.38.2.1

Abstract

D802N mutant gene of DNA Pol I ITB-1 was constructed and expressed in Escherichia coli. The crude enzyme was characterized and compared to that the wild-type. The optimum pH and temperature of the mutant was slightly differences with those the wild-type. The optimum pH and temperature of the mutant were 9.0 and 50oC, while the wild-type were 7.4 and 65oC, respectively. The differences of the above properties were followed by decreasing of specific activities of the mutant (1.34 unit/mg protein) 2.7 times lower to that the wild-type (3.71 unit/mg protein). Meanwhile the catalytic efficiency (kcat/Km ) of the mutant decreased 2 times lower compared to that the wild-type. Mutation at D802N of DNA pol I ITB-1 caused decreasing on the mutant affinity to the substrate and thus loss activity and instability of the enzyme.
Co-Authors Akhmaloka Akhmaloka F. Madayanti M. R. Moeis