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Marlia Singgih
School of Pharmacy, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132
Astronomy Chemistry Earth & Planetary Sciences Mathematics Physics

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Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis Elin Julianti; Susanti Susanti; Marlia Singgih; Laida Neti Mulyani
Journal of Mathematical and Fundamental Sciences Vol. 51 No. 2 (2019)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2019.51.2.6

Abstract

Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freeze-thawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.
Co-Authors Elin Julianti Laida Neti Mulyani Susanti Susanti