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Mudasir Mudasir
Department of Chemistry, Faculty of Mathematics and Fundamental Sciences, University of Gadjah Mada, Jalan Sekip Utara BLS 21, Bulaksumur, Daerah Istimewa Yogyakarta 55281
Astronomy Chemistry Earth & Planetary Sciences Mathematics Physics

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Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease Dhony Hermanto; Mudasir Mudasir; Dwi Siswanta; Bambang Kuswandi; Nurul Ismillayli
Journal of Mathematical and Fundamental Sciences Vol. 51 No. 3 (2019)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2019.51.3.8

Abstract

PEC of the alginate-chitosan membrane as supporting material for immobilizing urease was produced. This study aimed to develop a supporting material for enzyme immobilization that has high stability, a fast response time and an easy and relatively inexpensive preparation procedure. An alginate-chitosan PEC membrane was produced by reacting alginate hydrosol and chitosan (1:1 in mass) at pH 5.28, followed by mixing and drying at room temperature. The FTIR spectra, XRD patterns and SEM assay confirmed that alginate-chitosan PEC was obtained. The color change of the BTB indicator proved that urease was trapped in the cavities of the alginate-chitosan PEC membrane while the immobilized urease still showed catalytic activity. Thus, the membrane of alginate-chitosan PEC has good characteristics as a matrix for urease immobilization.
Co-Authors Bambang Kuswandi Dhony Hermanto Dwi Siswanta Nurul Ismillayli