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All Journal Journal of Agromedicine and Medical Sciences Indonesian Journal of Cardiology
M Aris Widodo
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Health Professions Medicine & Pharmacology

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Analysis of Low–level Cadmium Exposure Effects on HUVECs (Human Umbilical Vein Endothelial Cells) Cell Viability and Morphology Kristianningrum Dian Sofiana; Provisia Marthalita Y.W.; Khotimah Husnul; M Aris Widodo
Journal of Agromedicine and Medical Sciences Vol 5 No 1 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i1.9126

Abstract

Cadmium is a heavy metal that could be found in daily life. This metal has a toxicity, could contaminate the environment, and affect human health. The main aim of this research was to find the effect of low concentration Cadmium exposure in acute time toward HUVECs cell morphology and viability.In a True experimental research with in vitro model using HUVECs cell, HUVECs cell was divided into four groups. One control group (without CdCl2 induction) and three treatment groups with CdCl2 induction with various concentrations, 0,153 µg/L, 1,53 µg/L and 15,3 µg/L. The trial was repeated five times for each group. Cell morphology was observed with an inverted microscope. Cell viability was examined by MTT assay. Data were analyzed using Kruskal Wallis statistical test and continue with the Man Whitney test. Correlation test was using Spearman.Morphology of treatment group HUVECs cell induced by CdCl2 concentration 15,3 µg/L looked significantly different compared with control group (p<0.05). Cell viability on group HUVECs induced by CdCl2 15,3 µg/L significantly different compared with the control group. The correlation test resulted R= -0,665 with probability 0.001 which means the higher concentration of CdCl2 the lower the viability of cells. Cadmium in low concentration induces cell morphology change and reduce cell viability. Keywords: HUVEC, cadmium, cell morphology, cell viability.
Potensi Xanton Terhadap Status Oksidan dan Antioksidan pada Tikus Model Aterosklerosis Dwi Laksono Adiputro; M Aris Widodo; Rachmad Romdoni; Djanggan Sargowo
Jurnal Kardiologi Indonesia Vol. 33, No. 4 Oktober - Desember 2012
Publisher : The Indonesian Heart Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30701/ijc.v33i4.22

Abstract

Introduction.  Xanthones, which have potent antioxidative and antiinflammatory, and various other bioactivities,  are rich in a tropical fruit tree, mangosteen, Garcinia mangostana. The effect of xanthoneson oxidant level and antioxidant activity in hypercholesterolemic rats is still unknown.Objectives. To clarify an effect of xanthoneson oxidant level  and antioxidant activity in hypercholesterolemic rats.Methods. A total of 32 Wistar rats were divided into four groups (n=8), include control, hypercholesterolemic diet groups, hypercholesterolemic diet + xanthoneat dose 35; 70; and 140 mg/kg body weight. Control group receive standart diet for 60 days. Hypercholesterolemic diet group receive standart diet plus yellow egg, sheep oil, cholic acid, and pig oil for 60 days per oral. Analysis of blood hydrogen peroxide level, aorta glutathione peroxidase activity, and aorta catalase activity, were done using spectrophotometric. The ANOVA test were used to analyze the different levels of hydrogen peroxide, glutahione peroxidase activity, and catalase activity.Result. Hypercholesterolemic diet increase blood hydrogen peroxide level significantly compared to control group (p<0.05). Xanthone decrease blood hydrogen peroxide level significantly at all dose compared to control group (p<0.05). There is no significant difference of aorta glutahione  peroxidase activity in all groups (p>0.05). Xanthone modulate an activity of catalase in aorta at dose 140 mg/kg body weight.Conclusion. Xanthone has potency to decreaseblood oxidant level and modulate aorta catalase activity.
Co-Authors Djanggan Sargowo Dwi Laksono Adiputro Khotimah Husnul Kristianningrum Dian Sofiana Provisia Marthalita Y.W. Rachmad Romdoni