Noorhamdani Noorhamdani
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The extract of kemangi leaves as inhibitor of biofilm from Staphylococcus aureus in vitro Putu Sri Maharani Utami; Noorhamdani Noorhamdani; Masruroh Rahayu
Journal of Agromedicine and Medical Sciences Vol 6 No 3 (2020)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v6i3.17541

Abstract

Biofilm is a mechanism of bacterial defense against antimicrobials that can cause resistance. Staphylococcus aureus is a biofilm-producing bacteria and the most often cause of skin and soft tissue infections. Therefore, efforts are needed to prevent the formation of Staphylococcus aureus biofilms. Basil leaves are herbal plants that contain eugenol and tannin compounds, which are thought to inhibit the formation of biofilms. This research is a laboratory experimental study that aims to prove the effect of basil leaves ethanol extract (Ocimum sanctum) on the establishment of Staphylococcus aureus biofilms with in vitro method and determine the minimum inhibitory biofilm concentration needed. In this study, the tube method with 7 different concentrations was used. The results of biofilm ring formation obtained and measured quantitatively using Mean Gray Value in Adobe Photoshop CS6. From the study’s results, is found that the increase in extract concentration is directly proportional to the thinning of the biofilm ring on the tube with a minimum inhibitory concentration of biofilm at a concentration of 30%. The Pearson correlation test showed a very strong and significant correlation (r = 0.898, p = 0,000), and the Oneway ANOVA comparison test known a significant difference among the mean of each group (p = 0,000). From these results it can be known that the ethanol extract Ocimum sanctum can inhibit the formation of Staphylococcus aureus biofilms in vitro.
Perebusan dan penumisan menurunkan kandungan beta karoten dalam wortel Rany Adelina; Noorhamdani Noorhamdani; Annasary Mustafa
Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 1, NOMOR 3, SEPTEMBER 2013
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.757 KB) | DOI: 10.21927/ijnd.2013.1(3).164-168

Abstract

ABSTRACTBackground: Carrot is included in high beta carotene vegetable. Therefore, there were limited studies about the effect of cooking on nutrient content. Beta carotene found in carrots are provitamin A carotenoids that have strong antioxidant activity.Objectives: To determine the amount of beta carotene in carrots treated with two different cooking methods, that were, boiling and sautéing.Methods: A posttest–only control group design was used for this study. Samples of this study were local carrots varieties, and were selected by quota sampling. The carrots were divided into three groups, with fresh carrots (n=6) as control groups, and boiled carrots (n=6) and sautéed carrots (n=6) as treatment groups. Beta carotenes were extracted using petroleum ether-acetone solvents. They were separated by using column chromatography and measured by spectrophotometer at wave length 450nm. Data collected were analyzed with one way anova followed by post hoc tests duncan, independent t-test, andpaired t-test.Results: The level of beta carotene between fresh carrots, boiled carrots, and sauteed carrots have significant difference with p=0.013 (anova, p<0.05), whereas the level of beta carotene in boiled carrots and sauteed carrots have no significantly difference with p=0.376 (independent t-test, p>0.05). However,there is a significant decrease in the level of beta carotene in beginning and end groups from the boiling method with p=0.027, and the level of beta carotene in beginning and end groups from the sauteing method have a signifi cant decrease with p=0.020 (paired t-test, p<0.05).Conclusions: Boiling and sauteing decreased beta carotene levels in carrots.KEYWORDS: beta carotene, boiling, carrot, sauteeingABSTRAKLatar belakang: Wortel termasuk dalam sayuran dengan kandungan beta karoten yang tinggi. Walaupun demikian, studi mengenai pengaruh pemasakan terhadap kandungan gizinya masih terbatas. Beta karoten merupakan salah satu jenis karotenoid provitamin A dan berperan sebagai antioksidan kuat yang sangat bermanfaat bagi proses metabolisme yang terjadi pada tubuh manusia.Tujuan: Untuk mengetahui perbedaan kandungan beta karoten pada wortel yang diolah dengan perebusan dan penumisan.Metode: Penelitian ini menggunakan metode eksperimental rancangan acak lengkap. Desain penelitian ini adalah the posttest-only control group design. Populasi dan sampelnya adalah wortel varietas lokal. Sampel dipilih dengan cara kuota sampling untuk dibagi dalam tiga kelompok, yaitu wortel mentah (n=6) sebagai kelompok kontrol, sedangkan kelompok perlakuan adalah wortel yang direbus dengan air (n=6) dan wortel yang ditumis dengan minyak goreng (n=6). Variabel yang diukur adalah kandungan beta karoten pada masing-masing kelompok menggunakan kromatografi kolom dan spektrofotometri pada panjang gelombang 450 nm. Analisis data menggunakan oneway anova dilanjutkan dengan post hoc tests duncan, uji independent t-test, dan paired t-test.Hasil: Kandungan beta karoten antara wortel mentah, direbus, dan ditumis berbeda secara signifikan yaitu p=0,013 (anova, p<0,05). Kandungan beta karoten pada wortel direbus tidak berbeda secara signifikan dengan wortel ditumis yaitu p=0,376 (uji independent t-test, p> 0,05). Terbukti adanya perbedaan kandungan beta karoten pada kelompok awal dan akhir dari teknik pengolahan perebusan menurun secara signifikan yaitu p=0,027, sedangkan kandungan beta karoten awal dan akhir dari teknik pengolahan penumisan menurun secara signifikan yaitu p=0,020 (uji paired t-test, p<0,05).Kesimpulan: Perebusan dan penumisan berpengaruh terhadap penurunan kandungan beta karoten dalam wortel.KATA KUNCI: beta karoten, perebusan, penumisan, wortel