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Khoirul Muslih
Department Of Water Resource Management, University Bangka Belitung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Karakteristik asam amino dan kandidat bakteri probiotik produk fermentasi teritip (Anomia ephippium) di Bangka Ardiansyah Kurniawan; Khoirul Muslih
Journal of Aquatropica Asia Vol 1 No 1 (2014): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

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Teritip (Anomia ephippium) was well known by local people of Bangka Belitung Island as raw material for traditional fermented product called kecalo teritip. The aims of the study were to identify the content of amino acids and fermenting bacterias of the fermented product as a potential functional food. The steps of production process of the fermented product were the collecting of teritip sample from its substrate on the rocks along the beach, separating the whole edible part from the shells of teritip, and then the flesh were washed and salt-fermented. The amino acids of the fermented product were aspartic acid 2,59%, glutamic acid 3,24%, serine 0,55%, histidine 0,43%, glisine 1,69%, threonine 1,36%, arginine 0,96%, alanine 2,1%, tyrosine 0,37%, methionine 0,83%, valine 1,75%, phenilalanine 1,26%, isoleusine 2,15% dan lysine 1,59%. The identification of bacterias showed that there were five main bacterias in the product e.g Serratia marcescens, Acinetobacter baumannii, Actinobacillus sp., Bacillus cereus, dan Pseudomonas putida. High content of amino acids in the fermented product were suitable to be a functional food.
Sakarifikasi daun nanas (Ananas comosus) dengan lignoselulase dari bakteri endosimbion insang temilok (Bactronophorus thoracites) Khoirul Muslih; Denny Syaputra
Journal of Aquatropica Asia Vol 1 No 2 (2015): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

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Wood borer shellfish temilok (Bactronophorus thoracites) is a species of clams from family Teredinidae which used the wood as the main source of their food. The gill of temilok is inhabited by bacterias whom able to use the cellulose rich-fiber of the wood. The finding of bacterias which able to produce a cellulose-degrading enzymes was very usefull to improve the saccharification efficiency and effectivity of cellulose-rich material that comes from crops or papers from offices disposal. The aim of the research was to isolate the bacteria from the gill of temilok, and to know the saccharification kinetics of fiber of pineapple leafs by the secreted cellulase from the bacteria in the form of soluted glucose. Isolated bacterias was growth in a sterilized sea water (salinity of 20o/oo) at 16-20 oC without the addition of nitrogen sources to get the direct atmospheric dinitrogen-using bacteria. The interaction effects of incubation periods (24, 48, and 72 hours), and substrate concentrations (0,2%, 0,4%, and 0,6%) were investigated by measuring and calculating the concentrations of soluted glucose in the media using phenol-sulfate (phesul) method. Experimental design using factorial completed split plot, and data processing using Microsoft Excell. Variance analyses of soluted glucose concentrations showed that the interactions give no significant effects on soluted glucose consentrations in the media. The highest concentration was 88,1722 ppm (incubation period 72 h, substrate concentration 0,2%), and the lowest was 28,8340 ppm (incubation period 48 h, substrate concentration 0,2%).
Co-Authors Ardiansyah Kurniawan Denny Syaputra