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EVALUASI PENYEBAB HASIL INVALID PADA PEMERIKSAAN RT-PCR PASIEN COVID-19 Christina destri Wiwis Wijayanti; Sulistyowatiningsih Sulistyowatiningsih; Halik Wijaya
Jurnal SainHealth Vol 6, No 1 (2022): Maret 2022
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v6i1.1727.1-7

The COVID-19 pandemic has occurred in Indonesia since March 2020. An increase in cases requires the involvement of various medical elements from the beginning of the patient's condition to the diagnosis decision. Preparation of pre-analysis, analysis, to good post-analysis in the laboratory greatly supports the results of the diagnosis needed. The purpose of this study was to find out various factors that influence invalid results in a real-time examination – polymerase chain reaction (RT-PCR) in patients at Dr. Wahidin Sudirohusodo Mojokerto general hospital. Data collection from the hospital's biomolecular laboratory in a cross-sectional descriptive observational manner was taken from September to November 2020. A total of 40 data processed with descriptive statistics percentage showed a tendency to invalidity results by 30%. Pre-analytic errors play a big role in the RT-PCR method inspection process. Sample misidentification, poor sample quality, non-standard sample transportation conditions, and sample storage are some of the things that support the invalidity of the results of the COVID-19 RT-PCR examination method. Evaluation during the work process at various stages as well as the use of controls can minimize laboratory results that are not appropriate. Keywords : COVID-19, RT-PCR, Invalid

ANALISA BAKTERI TOTAL PADA BUAH PEPAYA (Carica Papaya L.) YANG DI STERIL MENGGUNAKAN METODE AUTOCLAVE Didik Purwanto; Anton Yuntarso; Christina Destri Wiwis Wijayanti
Jurnal SainHealth Vol 5, No 1 (2021): Maret 2021
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v5i1.901.25-29

Papaya (Carica papaya L.) is a plant that can be used as traditional medicine. All part of a papaya from the roots to the tips of the leaves have high medical value. The disadvantages of papaya is that if the ripe papaya fruit does not last long and rots easily. Total Plate Number is a number that shows the number of mesophyll bacteria or bacteria that live at 15Â°C - 55Â°C in every 1 ml or 1 gram of food samples examined. This study aims to determine how long the effect of the durability of papaya fruit that has been sterilized using autoclave and to determine the total number of bacteria in papaya fruit that has been sterilized using autoclave. Results of the study of papaya fruit samples that had been sterilized using autoclave on day 3, the ALT value was 0.67 Ã— 102 CFU/ml, so it can be said to meet the requirements according to SNI (30Â°C, 72 jam) 1 Ã— 102 colony/g. Day 4 to day 10 the value obtained was 1.17 â€“ 25.16 Ã— 102 CFU/ml where the value did not meet the requirements according to SNI.

PENGARUH AIR REBUSAN DAUN SEMANGGI AIR (Marsilea crenata) TERHADAP PENURUNAN KADAR GLUKOSA DARAH MENCIT (Mus musculus) HIPERGLIKEMIA Yenny Puspa Rini; Gilang Firmanda; Christina Destri Wiwis Wijayanti; Evy Ratnasari Ekawati
Jurnal SainHealth Vol 7, No 2 (2023): September 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i2.14353.63-67

Hyperglycemia is a marker of diabetes mellitus which is generally caused by damaged pancreatic ? cells which interferes with insulin secretion, causing the amount of blood glucose in the body to be uncontrolled. Marsilea crenata is known to contain several phytochemical compounds such as phenolics, flavonoids and steroids which have a positive effect on treating metabolic disorders. This study aims to determine the effect of Marsilea crenata cooking water on blood glucose levels in Mus musculus. The method used to check blood glucose levels is the POCT method. The results obtained in this study indicate that there is a difference in the average blood glucose examination in the treatment group and the control group. The average blood glucose before treatment was found to be 186.1 mg/dl, while measurements at the 4th and 7th hours after therapy with Marsilea crenata boiled water showed results of 166 mg/dl and 155.2 mg/dl. Statistical tests indicated that there was a significant difference or the effect of giving Marsilea crenata boiled water on decreasing blood glucose levels. Keywords : Marsilea crenata, Mus musculus, Blood glucose level, Hyperglycemia

PENGARUH AIR REBUSAN DAUN SEMANGGI AIR (Marsilea crenata) TERHADAP PENURUNAN KADAR GLUKOSA DARAH MENCIT (Mus musculus) HIPERGLIKEMIA Yenny Puspa Rini; Gilang Firmanda; Christina Destri Wiwis Wijayanti; Evy Ratnasari Ekawati
Jurnal SainHealth Vol. 7 No. 2 (2023): September 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i2.14353.63-67

Hyperglycemia is a marker of diabetes mellitus which is generally caused by damaged pancreatic ? cells which interferes with insulin secretion, causing the amount of blood glucose in the body to be uncontrolled. Marsilea crenata is known to contain several phytochemical compounds such as phenolics, flavonoids and steroids which have a positive effect on treating metabolic disorders. This study aims to determine the effect of Marsilea crenata cooking water on blood glucose levels in Mus musculus. The method used to check blood glucose levels is the POCT method. The results obtained in this study indicate that there is a difference in the average blood glucose examination in the treatment group and the control group. The average blood glucose before treatment was found to be 186.1 mg/dl, while measurements at the 4th and 7th hours after therapy with Marsilea crenata boiled water showed results of 166 mg/dl and 155.2 mg/dl. Statistical tests indicated that there was a significant difference or the effect of giving Marsilea crenata boiled water on decreasing blood glucose levels. Keywords : Marsilea crenata, Mus musculus, Blood glucose level, Hyperglycemia

PERBANDINGAN HASIL PEMERIKSAAN HBA1C METODE BORONATE AFFINITY DAN NEPHELOMETRY PHOTOMETRY: COMPARISON OF HBA1C EXAMINATION RESULTS BY BORONATE AFFINITY METHOD AND NEPHELOMETRY PHOTOMETRY Rizka Amalia; Christina Destri Wiwis Wijayanti; Setyo Dwi Santoso
Jurnal SainHealth Vol. 8 No. 1 (2024): Maret 2024
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v8i1.16509.15-19

HbA1C is glycosylated hemoglobin at the N-terminal group, HbA1C is hemoglobin with a minor component closely related to blood glucose. HbA1C is referred to as the breakdown of sugar in the body's metabolism or glycosylated hemoglobin or glycohemoglobin. HbA1C has several examination methods such as the Boronate Affinity method and Nephelometry Photometry. The two main concepts of HbA1C measurement are based on the separation of hemoglobin fractions and chemical reactions. Both of these methods offer a fast, simple, precise and accurate alternative to the currently used methods and provide substantial freedom from many common hassles. This type of research is comparative analytic experimental. In this study, 25 samples were used during the November-January 2023 period. The purpose of this study was to evaluate the results of the HbA1C examination using two methods. In this study it was concluded that based on the Independent T Test it showed the results of Sig 0.913 > ? (0.05) so that it could be concluded that there was no significant difference in the results of the two examination methods Keywords : HbA1C, Boronate Affinity, Nephelometry Photometry

Analisis Laju Endap Darah dan C-Reaktif Protein Metode Ifa pada Neonatus dengan Riwayat Ketuban Pecah Dini Rara Tarika; Christina Destri Wiwis Wijayanti; M. Sungging Pradana; Esti Rizkiana Pratiwi; Stefani Widodo
Jurnal Ners Vol. 10 No. 2 (2026)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v10i2.54997

Ketuban Pecah Dini (KPD) meningkatkan risiko infeksi neonatal yang mengancam jiwa, sehingga deteksi dini proses inflamasi sangat krusial. Penelitian ini menganalisis nilai Laju Endap Darah (LED) dan kadar C-Reaktif Protein (CRP) menggunakan metode Immunofluorescence Assay (IFA) pada neonatus dengan riwayat KPD. Metode penelitian yang digunakan adalah observasional deskriptif analitik dengan rancangan cross-sectional terhadap 50 neonatus (25 laki-laki dan 25 perempuan) di Rumkit Tingkat IV Gubeng. Hasil penelitian menunjukkan bahwa median nilai LED pada neonatus perempuan (17 mm/jam) dan laki-laki (16 mm/jam) tidak memiliki perbedaan signifikan (p = 0,558). Demikian pula, kadar CRP tidak menunjukkan perbedaan signifikan berdasarkan jenis kelamin (p = 0,207). Temuan krusial menunjukkan hubungan antara LED dan CRP bersifat sangat lemah dan tidak signifikan secara statistik (rho = 0,19; p = 0,178). Simpulan dari penelitian ini adalah meskipun KPD memicu peningkatan biomarker inflamasi di atas ambang batas normal, profil tersebut tidak dipengaruhi oleh gender dan kedua biomarker tidak bergerak secara linier. Hal ini menegaskan pentingnya penggunaan kedua parameter secara komplementer dalam skrining infeksi neonatal.

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