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Karakterisasi Lima Isolat Cendawan Endofit Tanaman Padi Sebagai Agen Antagonis Pyricularia Oryzae Ika Nur Fatimah; Tunjung Pamekas; Hartal Hartal
PendIPA Journal of Science Education Vol 4, No 3 (2020): JULY - OCTOBER
Publisher : University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.131 KB) | DOI: 10.33369/pendipa.4.3.1-6

Abstract

Endophytic fungi are microorganisms that live in plant tissues or plant organs, such as seeds, leaves, flowers, stems and roots which are able to induce plant resistance to diseases. Local isolate of endophytic fungi are expected more effective to control diseases. Blast disease, that caused by Pyricularia oryzae, is a limited factor on rice paddy production. This study aim was to characterize dan select endophytic fungi isolates isolated from rice paddy as antagonistic agents to control P. oryzae. The research methods were survey rice paddy in the field, isolation and characterization of endophytic fungi isolates, isolation and characterization of P. oryzae, and testing the mechanism of antagonism of endophytic fungi isolates and P. oryzae in vitro by dual culture test. The results showed that there were five endophytic fungi isolated from rice paddy, that were Aspergillus candidus, Gliocladium sp, Trichoderma harzianum, Penicillium sp, and Nigrospora sp. The eight isolates of endophytic fungi were isolated from various sites having different micro environment. Endophytic fungi isolated from rice paddy at the district of Central Bengkulu demonstrated highest inhibition percentage, which was 85.60% with competition for space, nutrition, and oxygen, antibiosis, and hyperparasitism.
Mode of Actions and Pathogenicity of 11 Endophytic Fungi on Fusarium oxysporum Restu Aminningsih; Tunjung Pamekas; Hendri Bustamam
AGRITROPICA : Journal of Agricultural Sciences Vol 4, No 2 (2021)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/j.agritropica.4.2.122-128

Abstract

Fusarium oxysporum is the pathogenic cause of the disease on chili plants that can reduce production in the cultivation process that needs to be done to control an environmentally friendly manner using endophytic fungi. This study aims to test the inhibition, the mechanism of antagonism, and pathogenicity of eleven endophytic fungi origin of pepper plants Bengkulu province against the pathogen Fusarium oxysporum in vitro. Research conducted in the Laboratory of Plant Protection, Faculty of Agriculture, University of Bengkulu from December 2020 to February 2021. The study consists of: the rejuvenation of the fungi isolates of endophytic and fungi pathogens Fusarium oxysporum, dual culture test, the mechanism of antagonism test, as well as pathogenesis endophytic fungi test. Endophytic fungi isolate obtained from the collection of the laboratory of Plant Protection, University of Bengkulu. The results of the research show from eleven endophytic fungi only 1 endophytic fungi which has a percentage of inhibition of 50% of the isolates of Rhizoctonia sp.2. The mechanism of antagonism occurs consisting of the competition between the space of nutrients and oxygen, hiperparasitisme, and antibiosis. patogenensitas test show only isolates of Rhizoctonia sp.2 that do not produce patches on the chili seeds.