Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Alotrop Jurnal Pendidikan dan Ilmu Kimia Current Research on Biosciences and Biotechnology
Yosie Andriani
Institute of Marine Biotechnology, Universiti Malaysia Terengganu
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
STUDY ON Hibiscus tiliaceus LEAVES AS ANTIBACTERIAL AND ANTIOXIDANT AGENTS Mohamad Syahmie Samsudin; Yosie Andriani; Purbowatiningrum Ria Sarjono; Desy Fitrya Syamsumir
ALOTROP Vol 3, No 2 (2019)
Publisher : University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/atp.v3i2.9874

Abstract

This study aimed to evaluate antioxidant and antibacterial activities of methanol, hexane, dichloromethane, ethyl acetate, and water crude sample fractions of H. tiliaceus leaves. Various type of samples play an important role to determining which compound that will give the positive results in antioxidant test (inhibiting free radical scavenging activity) which they probably have potency for preventing the cancer disease. Furthermore, those types of samples were also tested to the antibacterial test as their possibilities to be created as new antibiotics in the future. This research also focused on what compound groups that actually present in H. tiliaceus leaves by using the phytochemistry test. Antioxidant potency of H. tiliaceus leaves extracts for the radical scavenging activity for quantitative assay was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The antibacterial potency was measured by the Muller Hinton Agar (MHA) by applied the sample chemical crude in the plate. The result showed that all chemical crude of H. tiliaceus leaves has high antioxidant property as methanol crude (MC) 308.416%; hexane crude (HC) 232.837%; dichloromethane crude (DC) 150.837%; ethyl acetate crude (EC) 73.623% and water crude (WC) 71.777% with respected to the readings of  100% from quercetin (Q) as a positive control. From antibacterial result, entire samples had shown the positive results towards both gram positive (Staphylococcus aureus and Bacillus subtilis) and gram negative bacteria (Klebsiella pneumonia and Escherichia coli). The ranges of the inhibition zone were both at 7-10 mm and 10-15 mm. The phytochemistry test determine that the samples actually contained a lot of compounds which were proteins, carbohydrates, phenols/tannins, flavonoid, terpenoids, glycosides and also steroid. It is clearly indicated that H. tiliaceus leaves have potential to be used against the antioxidant and also as new antibacterial agents
Antioxidant activity and cytotoxicity property of extracts from various coastal plants against HepG2 cell lines Leong Kai Vyn; Murni Nur Islamiah Kassim; Desy Fitrya Syamsumir; Hermansyah Amir; Ismiyarto; Yosie Andriani
Current Research on Bioscences and Biotechnology Vol. 1 No. 2 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/10.5614/crbb.2019.1.2/ONXN6178

Abstract

Reports have shown an upward trend for liver cancer in recent years. It is also one of the deadliest cancers globally due to its complexity in detection and treatment. Hence, there is an urgency todevelop the anti-liver cancer agent from natural resources which is highly effective with minimum side effects. This study aimed to evaluate the antioxidant activity and cytotoxicity property of extracts with different polarity solvents (hexane and methanol) obtained from coastal plants collected from Merang, Terengganu. Three species (Melaleuca leucadendra, Terminalia catappa, and Rhodomyrtus tomentosa) were chosen due to their abundance and lacking anticancer studies performed on them. Green leaves were collected directly from the trees and extracted using hexane and methanol successively. Preliminary phytochemical tests (phenolic, flavonoid, terpenoid, saponin, alkaloid and glycoside) were performed on the extracts, followed by an antioxidant test based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Methanol extracts with high antioxidant property were continued for cytotoxicity study on HepG2 cell lines by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that all methanol extracts from all plants showed high antioxidant property (IC50: 0.83-1.62 mg/ml) and moderately cytotoxic activity (IC50: 24.7-28 μg/ml) against HepG2 cell lines. In contrast, hexane extracts showed very weak antioxidant activities. The highest activity was obtained by methanol extract of M. leucadendra (MLM), followed by T. catappa (TCM) and R. tomentosa (RTM), respectively. These promising results indicated that MLM could be a potential candidate for further study related to the antioxidant and cytotoxicity on HepG2 cell lines, such as for anti-liver cancer agent.
Co-Authors Desy Fitrya Syamsumir Desy Fitrya Syamsumir Hermansyah Amir Ismiyarto Ismitarto Leong Kai Vyn Mohamad Syahmie Samsudin Murni Nur Islamiah Kassim Purbowatiningrum Ria Sarjono