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DONATA S. PANDIN
Balai Penelitian Tanaman Kelapa dan Palma Lain, Manado
Agriculture, Biological Sciences & Forestry

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Keragaman Genetik Kelapa Dalam Bali (DBI) dan Dalam Sawarna (DSA) Berdasarkan Penanda Random Amplified Polimorphic DNA (RAPD) DONATA S. PANDIN
Buletin Palma No 37 (2009): Desember, 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.419 KB) | DOI: 10.21082/bp.v0n37.2009.152-165

Abstract

Genetic Diversity of Bali Tall (DBI) and Sawarna Tall (DSA) based onRandom Amplified Polymorphic DNA (RAPD)Genetic Diversity of Bali Tall (DBI) and Sawarna Tall (DSA) based on Random Amplified Polymorphic DNA (RAPD). Ten Plants were used in each population. The objective of this research were to determine genetic diversity within- and inter-population of Bali Tall (DBI) and Sawarna Tall (DSA) coconuts, and genetic relationship of those population based on RAPD ( ). Research was done in Plant Biology Laboratory of CenterResearch of Genetic Resources and Biotechnology, Institut Pertanian Bogor, February – May 2007. DNA extraction was done by modified Rohde method and to determine the concentration and quality of DNA by Sambrook method. Ten RAPD 10-mer were used namely OPA-02, OPA-08, OPA-10, OPA-13, OPA-20, OPB-08, OPB-11, OPB-12, OPB-15, OPB-20. Genetic relationship between individuals of DBI and DSA coconut populations based on NTSys ver.2.0 programe (Program Numerical Taxonomy and Multivariate Analysis), and clustering analysis based on UPGMA method. These ten RAPD primers used could separate DBI and DSA in each group. Genetic diversity within-population of Bali Tall coconut population range from 2.4% to 30.7% with average 21.7%. So that, opportunity to improve characters in DBI coconut population could be done by selection. Genetic diversity within-population of Sawarna Tall coconut population progressively narrow, range from 1.5% to 12.4% with average 12.7%, so that selection in order to character improvement in this population could be done selectively. Genetic relationship between DBI dan DSA populations far enough (54%), so that crossing between those population will be good for characters improving.
Keragaman Genetik Kultivar Kelapa Dalam Mapanget (DMT) dan Dalam Tenga (DTA) Berdasarkan Penanda Random Amplified Polymorphic DNA (RAPD) DONATA S. PANDIN
Buletin Palma No 36 (2009): Juni, 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v0n36.2009.17-29

Abstract

Genetic Diversity of Mapanget Tall (DMT) and Tenga Tall (DTA) Coconuts based on Random Amplified Polymorphic DNA (RAPD)The objective of this research were to determine genetic diversity within and inter population of Mapanget Tall (DMT) and Tenga Tall (DTA) coconuts, and their genetic relationship based on RAPD ( ). Research was done at Plant Biology Laboratory of Center Research of Genetic Resources and Biotechnology, Institut Pertanian Bogor, from February to May 2007. The result showed that ten RAPD 10-primer used could separate DMT and DTA in each group. Genetic diversity within population was relatively narrow, so that selection in order to improve character in each population must be done selectively. Genetic relationship between DMT dan DTA populations was high (52%), so that crossing between these population will give highest heterosis effect.
Viabilitas Polen Aren pada Media Buatan DONATA S. PANDIN; ELSJE T. TENDA
Buletin Palma No 39 (2010): Desember, 2010
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v0n39.2010.190-196

Abstract

Sugarpalm Pollen Viability on Artificial MediaResearch conducted at the Plant Breeding Laboratory of Indonesia Coconut and Other Palmae Research Institute (ICOPRI) Manado, from February until April 2010. The research aims to find ways of processing sugarpalm pollen and testing techniques of pollen viability. The materials used were male flowers of the Tall type of sugarpalm. Pollen viability test carried out using a completely randomized design with 12 treatments of 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 hours after sown on growth media, and repeated three times. The results showed that the sugarpalm male flower drying good for the process of pollen is to use an oven or a dehumidifier at a temperature of 35 o C - 37 o C for 30 hours. The yield of sugarpalm pollen produced an average of 1.41%. The results showed that pollen viability of sugarpalm not germinated until 12 hours after sowing, and just started to sprout after 14 hours with the percentage increasing until 24 hours after grown. Sugarpalm highest pollen viability was found on treatment after 24 hours of observation.
Inbreeding Depression on Morphological Markers in Mapanget Tall Coconut Line No. 32 DONATA S. PANDIN
Buletin Palma No 36 (2009): Juni, 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v0n36.2009.30-39

Abstract

The objective of this research was to determine inbreeding depression based on morphological markers in Mapanget Tall coconut line No.32. The result showed that inbreeding depression for vegetative characters in S2 generation was expressed only in girth of stem 100 cm from soil (3.28%) and width of leaflet (1.17%). Inbreding depression in S3 generation expressed in girth of stem 20 cm and 100 cm from soil (13.01% and 8.03%), height of 11 leafscars (11.80%), and number of leaflet (6.15%). In S4 generation , inbreeding depression expressed in girth of stem 20 cm and 100 cm from soil (5.39% & 14.41%), height of 11 leafscars (17.40%), width of leaflet (4.87%), and number of leaves (13.62%). Inbreeding depression also expressed in characters number of inflorescence per palm per year (7.41%), length of inflorescence stalk (34.18%), number of female flowers (21.32%), and length of male flowers bunch (10.84). In S3 generation inbreeding depression expressed in characters number of inflorescence per palm per year (14.90%), number of fruit per inflorescence (11.51%), length of inflorescence stalk (38.17%), number of female flowers (47.91%), and length of male flowers bunch (5.31%), but not expressed in width of inflorescence stalk (-11.07%) and thick of inflorescence stalk (-8.73%). Inbreeding depression expressed in S4 for all generatif characters ranged from 29.09% to 85.92%. In fruit components, inbreeding depression in S2 expressed in total fruit (9.01%), coconut water (16.07%), coconut shell (8.41%), and endosperm (7.55%) except on coconut fiber (-1.03%). In S3, inbreeding depression expressed in total fruit (5.20%), coconut fiber (0.58%), and endosperm (6.37%), except on coconut shell (-2.05%) and coconut water (-4.50%). In S4, inbreeding depression expressed in all characters of fruit component, total fruit (47.68%), coconut fiber (45.86%), coconut water (4.00%), coconut shell (51.82%), and endosperm (54.04%). Due to inbreeding depression, morphological performance of S4 generation nearly the same to that of Dwarf coconut.
Depresi Silangdalam Kelapa Dalam Mapanget Berdasarkan Penanda Mikrosatelit (SSR) DONATA S. PANDIN
Buletin Palma No 37 (2009): Desember, 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v0n37.2009.127-137

Abstract

Inbreeding Depression Analysis Based on Microsatellite (SSRs)Markers in Mapanget Tall CoconutThe objective of this research was to determine inbreeding depression based on microsatellite (SSR) markers of second, third, and fourth generations of selfed Mapanget Tall coconut No.32 (DMT-32). The research was conducted in Plant Biology Laboratory, Research Center of Plant Genetic Resources and Biotechnology, Institut Pertanian Bogor. The research was done since May 2004 until May 2007. Fifteen out of 19 microsatellite ( , SSR) loci (primers) were used to analyze inbreeding depression in each generation of selfed Mapanget Tall coconut No.32. Based on 15 loci of microsatellite (SSRs) markers, showed that inbreeding depression expressed and have tendency to increase from generation to next generation of selfed Mapanget Tall coconut No.32 (DMT-32). Inbreeding depression in DMT-32 S2 (2.78%), DMT-32 S3 (10.54%), and DMT-32 S4 (15.74%). Four out of 15 microsatellite loci used were not in Hardy-Weinberg equilibrium, means that in DMT-32 S4 those loci were homozygote that’s why alleles of those loci were not inherits in equilibrium. DMT-32 S4 coconut can be used as plant materials to find progeny with higher heterosis level.
Co-Authors ELSJE T. TENDA