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All Journal VALENSI Jurnal Sains Dasar Al Ilmi Biota Bio Ilmi: Jurnal Pendidikan Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya
Elfira Rosa Pane
Fakultas Tarbiyah Dan Keguruan UIN Raden Fatah
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics

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MUTASI GEN KATG ISOLAT KLINIS L18 Mycobacterium tuberculosis RESISTEN ISONIAZID Pane, Elfira Rosa
Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam Sainmatika Volume 9 No. 1 Juni 2012
Publisher : Faculty of Mathematics and Natural Science, Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/sainmatika.v9i1.751

Abstract

Tuberkulosis (TB) merupakan penyakit infeksi menular yang disebabkan oleh Mycobacterium tuberculosis. Penyakit ini dapat diobati dengan obat-obat anti-TB (OAT). Resistensi bakteri TB terhadap OAT dapat dipicu oleh pengobatan TB yang tidak tuntas. Badan kesehatan dunia (WHO) telah mendefinisikan ‘Multi-drug resistant M. tuberculosis’ (MDR-TB) adalah jenis M. tuberculosis yang resisten setidaknya terhadap dua macam obat, yaitu rifampin (RIF) dan isoniazid (INH), yang merupakan OAT lini pertama. Penelitian sebelumnya terhadap sampel klinis L18  yang fenotipenya MDR-TB dan berdasarkan uji PCR isolat tersebut mengalami mutasi pada gen rpoB kodon 526 dan 531, tetapi tidak termutasi pada gen katG kodon 315. Tujuan riset ini adalah untuk mendapatkan informasi pada tingkat genotipe penyebab resistensi terhadap INH dalam isolat klinis MDR-TB tersebut. Tahapan penelitian yang dilakukan di sini yaitu Polymerase Chain Reaction (PCR) multipleks spesifik alel katG dan elektroforesis gel agarosa untuk semua isolat menunjukkan adanya dua pita DNA berukuran 0,43kb dan 0,29kb, penentuan urutan nukleotida(sekuensing) dibandingkan dengan fragmen yang sama dari M.tuberculosis galur standar H37Rv, dan analisis in silico menunjukkan isolat tersebut(L18) termutasi pada posisi 946, G menjadi T(G946T) terletak pada kodon 316, GGC menjadi TGC, mengakibatkan asam amino glisin termutasi menjadi sistein. Simulasi struktur ruang katalase peroksidase dengan program Pymol menunjukkan residu asam amino 316 berada dekat dengan sisi aktif pengikatan INH. Penelitian sebelumnya telah membuktikan bahwa mutasi pada residu 315 mengakibatkan sifat resistensi terhadap INH. Isolat ini tidak mengalami mutasi pada residu 315 sehingga mutasi pada residu 316 diduga kuat menjadi penyebab sifat resistensi.
MUTASI GEN Kat G PADA ISOLAT KLINIS R2 FENOTIPE MULTI DRUG RESISTEN Mycobacterium Tuberculosis Pane, Elfira Rosa
Al 'Ilmi : Jurnal Pendidikan MIPA Vol 1 No 1 (2012): Al 'Ilmi : Jurnal Pendidikan MIPA
Publisher : Universitas Islam Negeri Raden Fatah

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Abstract

Tuberculosis (TB) is an infected diseased caused by Mycobactenum tuberculosis, and tiratmeni with anti-tuberciulosis drugs (OAT) could cuird the disease. Inappopriate medication could cause TB drugs resistance. Multi-drug Resistant TB (MDR-TB) describes strains of tuberTrulosis that are resistant to at least the two main first line TB drugs - isoniajd (INH) and rifamticin (R1F), (World Health Orgariation). The am of our researth is to find the genotipe information about The caused of resistece INH in clinical isolate R2. The methods used in our research is Polymertuse Chain Reaction (PCR) muhiplex specific alleles kat G and electroporesis gel agarose all isolates produced two multiplex PCR 0,43kb and 0,29kb bands. Sequencing produce electroforegnim 0,43kb frament gene katG compared to the same frment M. tuberculosis wild tipe. Homology analsis showed isolate R2 have mutation in nucleotide 869, C to T. Data anasis obtained here thowed that C869T mutation in isolate R2 located in codon 290, GCT change in to GTT, and the consequence is amino acid alanin replaced by valin. Pymol program show amino acid residue 290 located in loop region N terminal and attmximately from the actize site. The  effect of this mutation and the relation in resistance INH bar no yet known. The implication of our results is to give the new information about mutation position in kat G gene M tuberculosis which is resistance INH because of mutation C869T (alanin29Ovalin) in isolate R2 hate not been published before. 
Uji Aktivitas Senyawa Antioksidan dari Ekstrak Metanol Kulit Pisang Raja (Musa paradisiaca Sapientum) Elfira Rosa Pane
Jurnal Kimia Valensi Jurnal Valensi Volume 3, No.2, November 2013
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v3i2.502

Abstract

AbstrakTelah dilakukan penelitian uji aktivitas senyawa antioksidan dari ekstrak metanol kulit pisang raja (Musa paradisiaca Sapientum). Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut metanol. Ekstraksi bertingkat dilakukan terhadap ekstrak metanol dengan n-heksan dan etil asetat. Dari uji fitokimia terhadap fraksi metanol menunjukkan positif flavonoid. Pengujian aktifitas antioksidan dilakukan dengan reaksi oksidasi asam linoleat dengan metoda feritiosianat (FTC) 0,05%. Sebagai standar antioksidan digunakan Butil hidroksianisol (BHA). Hasil penelitian ini menunjukkan ekstrak metanol, fraksi n-heksan dan etil asetat memiliki aktifitas antioksidan yang tidak jauh berbeda dengan BHA. Sedangkan fraksi etil asetat memiliki aktifitas antioksidan yang lebih tinggi daripada BHA.Kata kunci : antioksidan, feritiosianat, Musa paradisiaca SapientumAbstractA research has been done to evaluate the antioxidant activity of metanol extract Musa paradisiaca Sapientum peel’s. Maseration technique was used to get the extract using metanol as solvent. The metanol extract of the peel was re-extracted by solvents into n-hexan and ethyl acetate fractions. Phytochemical screening of metanol extract showed positive flavonoid. The antioxidant activities were tested by using ferric thiocyanate method 0,05% (FTC) on linoleic acid and buthyl hydroxyanisole (BHA) as antioxidant standard. Metanol extract, n-hexan and ethyl acetate fraction exhibited antioxidative activity that was not significantly different from BHA, on the other hand, the ethyl acetate fraction exhibited significant antioxidative activity, which is better than BHA.Keywords : antioxidant, ferric thiocyanate, Musa paradisiaca Sapientum
PENGARUH PENGOLAHAN BUAH MANGGA MANALAGI SEGAR (Mangifera indica L.) MENJADI MANISAN MANGGA KERING TERHADAP KADAR VITAMIN C Choriun Niswah; Elfira Rosa Pane; Ela Irmawati
Biota Vol 2 No 2 (2016): Vol 2 No 2 Agustus 2016
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

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Abstract

Manalagi  mango fruit (Mangifera indica L)  is one of fruits containing lots of vitamin C and it can be processed into mango dried candied. This research is aimed to determine the levels of vitamin C in the mango dried candied that is sold in modern markets in Palembang and to contribute about biology subject at senior high school / madrasah aliyah. The methods of this study is the idiometri test. This study uses the form control manalagi fresh mango fruit. Samples are taken from several modern market in Palembang city by using simple random sampling method. The result of the test iodimetri along with qualitative test on sample 4 and 6 do not contain vitamin C, while in sample 1, 2, 3, and 5 contain vitamin C which is an average of the levels of vitamin C in the sample 1 of 4.78 mg / 100 gr, 2 sample of 3.66 mg / 100 g, 3 sample of 2.64 mg / 100 g, 5 sample of 5.22 mg / 100 g. This shows manalagi fresh mango fruit is processed into dried candied still have high levels of vitamin C which is quite high when compared to the levels of vitamin C in control in the amount of 3.08 mg / 100 g. Contributing to education that is presented in the form of lesson plans, worksheets and posters. The contribute of  biology subject to the education is presented in the form of  lesson plans, worksheets and posters
UJI KANDUNGAN SIKLAMAT PADA LEGEN JAMU GENDONG DI KELURAHAN SEKIP JAYA PALEMBANG Irham Falahudin; Elfira Rosa Pane; Asri Arum Sari
Biota Vol 2 No 2 (2016): Vol 2 No 2 Agustus 2016
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

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Abstract

Legen is neutralizing bitter taste of herbs commonly used by herbalist. The sweet taste at the legen suspected of using artificial sweeteners. Cyclamate is one of the most common artificial sweetener used in food or beverages. In the world of education cyclamate included in class VIII SMP biology syllabus. The purpose of this research is in the legen contained cyclamate or not and the application of the experimental method (practicum) legen testing on student learning outcomes. Cyclamate content test study conducted in June 2015 and the application of the experimental method in August 2015. The method used in the testing of cyclamate is a deposition method while in the learning process used an experimental method (practicum). The results show that all samples tested legen containing cyclamate and exceeding the threshold is an average of the sample 1 (6 g / L), sample 2 (5.33 g / L), sample 3 (5.46 g / L), samples 4 (8.93 g / L) and sample 5 (6.26 g / L) while research using experimental method (practicum) menunjukka F count> F table (3.73> 2.66). The conclusion from this research that all traders use cyclamate exceeds the specified threshold and use of the experimental method affects the learning process on learning outcomes.
UJI KANDUNGAN BORAKS PADA PEMPEK LENJER YANG DIJUAL DI KELURAHAN PAHLAWAN Irham Falahudin; Elfira Rosa Pane; Niar Kurniati
Biota Vol 2 No 2 (2016): Vol 2 No 2 Agustus 2016
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

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Abstract

Pempek is processing product of fish meat and tapioca flour. Pempek has a low shelf life because the process of making it that is boiled first, it makes pempek easy to be slimy. It is one of the reason why the Pempek seller uses dangerous preservative such as borax. The purpose of this research is to know whether Pempek Lenjer that is sold in Pahlawan Palembang Village contains borax or not,  and contribution in the form of student worksheet (LKS) and poster. This research uses organoleptic test, then color test by using tumerik paper to know the existence of  borax in Pempek Lenjer at sold in Pahlawan, Kilometer 3,5, Palembang City, South Sumatera, Indonesia. Date research on 17-24st June 2015.  In this research, organoleptic test indicates that there is no Pempek Lenjer that has a characteristic containing borax, on the colour test all sampels lenjer pempek doesn’t contain borax, because it does not change color on paper tumerik that has dripped samples, performed three, and it is done validation in the student worksheet (LKS) with 5 validators from 3 schools and the result of validation of the student worksheet (LKS) from 5 validators is valid
PENGARUH LAMA PENYIMPANAN DAN KONSENTRASI NATRIUM BENZOAT PADA SUHU BERBEDA TERHADAP KADAR VITAMIN C CABAI MERAH (Capsicum annuum L.) DAN SUMBANGSIHNYA PADA MATERI ZAT-ZAT MAKANAN DI KELAS XI MA/SMA Zainal Berlian; elfira Rosa Pane; mardiana mardiana
Bioilmi: Jurnal Pendidikan Vol 1 No 1 (2015): Bioilmi: Jurnal Pendidikan
Publisher : Program Studi Pendidikan Biologi Fakultas Ilmu Tarbiyah dan Keguruan Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/bioilmi.v1i1.1125

Abstract

Red chilli (Capsicum annuum L.) is one of vegetables that useful for Indonesian people. Red chilli contains high vitamin C (ascorbic acid) and beta-carotene that surpass fruits often consumed by people like papaya, mango, pineaple and watermelon. Red chilli easily rotten so scientist use sodium benzoate to preservate anti-fungi. Red chilli which use sodium benzoate stored in 10oC (freezer) with variate in one day, 5 days and 10 days saving. This research point is to know the impact of sodium benzoate, saving time, saving in the room teperature and saving in the freezer to the red chilli vitamin C level. This research is held in the Politeknik Kesehatan Palembang Jurusan Gizi Laboratory. The decision of the vitamin C level based on the iodin titration. This research used completely randomized design with 8 treatments, 3 replications and 3 saving times till we get 72 trials. These treatments are A1= 0%/100g, A2= 0,07%/100g, A3= 0,09%/100g, A4 = 0,1%/100g, B1= 0%/100g, B2= 0,07%/100g, B3= 0,09%/100g, dan B4= 0,1%/100g which each saved one day, 5 days and 10 days in the room teperature and freezer. This research result show that saving time treatment have real impact to the red chilli vitamin C level is F count 238,57 more than 3,30 in the F table. In the adding concentration treatment sodium benzoate doesn‟t have the real impact to the red chilli vitamin C level that F count 0,023 less than 3,49 in the F table. The temperature treatment doesn‟t also have the real impact to the red chilli vitamin C level that F count -0,0008 less than the 18,51 in the F table. This research higest vitamin C level in the adding sodium benzoate 0,07% treatment which save in the room temperature since from H0 until H+10 the vitamin C level is increassy.
Pengaruh Penambahan FAME Terhadap Parameter Density, Distillation, dan Calculated Cetane Index Pada Produk Biosolar Oktari, Tantri; Pane, Elfira Rosa; Nugraha, Aji
Jurnal Sains Dasar Vol 13, No 1 (2024): April 2024
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v13i1.65692

Abstract

The fossil-based fuel energy crisis in Indonesia occurred because of the high demand to meet the demand for fuel in the transportation sector but the low availability of fossil fuels which are difficult to renew. One way to overcome this is to develop alternative energy sources to reduce the use of non-renewable energy sources such as diesel fuel. The government has determined the use of alternative energy sources such as biodiesel B30 which is a mixture of diesel fuel with a FAME (Fatty Acid Methyl Esters) content of 30%. However, there is a possibility that Indonesia will increase the FAME content. The method used is density 15˚C using ASTM D-1298, distillation using ASTM D-86 and calculated cetane index using ASTM D-4737. The results showed an increase in the value of samples B0, B30, B35, and B100, obtained at a density of 15˚C, namely 0.8371 gr/ml; 0.8484 gr/ml; 0.8505 gr/ml; 0.8729 gr/ml, in distillation the boiling point increases from IBP (Initial Boiling Point) to the end point, and the CCI is 48.4; 50.0; 50.6; 58.2.
PENGGUNAAN PRIMER GEN CYTOCHROME OXIDASE 1 DALAM REAKSI POLYMERASE CHAI REACTION (PCR) UNTUK IDENTIFIKASI KANDUNGAN BABI PADA MAKANAN Pane, Elfira Rosa; Legasari, Leni; Mardini, Intan
Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya Vol. 6 No. 2 (2024): Penelitian Kimia 2024
Publisher : Program Studi Kimia, Fakultas MIPA, Universitas PGRI Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/jc.v6i2.3532

Abstract

Muslims make up the majority of the population in Indonesia so halal food safety is of paramount importance. From a religious and health perspective, adulteration and contamination of Sus scrofa meat is considered a violation of halal norms. Sus scrofa is often used to adulterate beef to take advantage of the physical resemblance and price difference. The need to identify Sus scrofa meat contamination in food products was highlighted in this study. After a successful isolation process, the Polymerase Chain Reaction (PCR) method was used to validate the presence of porcine DNA. PCR utilizes primers specifically made to replicate specific DNA sequences associated with pigs. PCR testing provides precise results for pig DNA, ensuring the accuracy of detection of pork contamination in foodstuffs. This research contributes to efforts to maintain halal food safety in Indonesia by emphasizing the important role of PCR in the detection process of Sus scrofa meat.
Co-Authors Aji Nugraha Asri Arum Sari Choriun Niswah Ela Irmawati Irham Falahudin Leni Legasari mardiana mardiana Mardini, Intan Niar Kurniati Oktari, Tantri Zainal Berlian