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The use of formaldehyde protected palm kernel meal and its effects on animal performance, nitrogen utilization and unsaturated fatty acid composition in Priangan sheep Wiryawan, K.G; Parakkasi, A; Priyanto, R; Nanda, I.P
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 4 (2007)
Publisher : Indonesian Animal Sciences Society

An experiment was conducted to investigate the effect of formaldehyde-treated Palm Kernel Cake (BIS) in male Priangan sheep diet and its effect on animal performance, N utilization, and unsaturated fatty acids composition of musculus longisimus dorsal. The experiment was designed based on completly randomized design with four treatments and nine replications. The treatments consisted of four levels of formaldehyde treated BIS: R1 (0%), R2 (15%), R3 (30%) and R4 (45%). Results indicated that formaldehyde treated BIS up to 45% in diet did not negatively affect consumption, dry matter digestibility and daily live weight gain. Formaldehyde protection especially in R4 could significantly reduce ruminal N-NH3 concentration, increased protein digestibility and nitrogen retention, but did not influence allantoin urine concentration. Unsaturated fatty acid composition of musculus longisimus dorsal was not affected by formaldehyde treated BIS in diet. It is concluded that the inclusion of 45% formaldehyde treated BIS in diet did not negatively affect animal performance, and R4 is the most effective in improving nitrogen utilization in male Priangan sheep, but unable to alter the unsaturated fatty acids composition of musculus longisimus dorsal. Key Words: Palm Kernel Cake, Formaldehyde, Performance, Nitrogen, Unsaturated Fatty Acids

In vitro slow-release urea contained in rice straw-based diets to increase efficiency of rumen microbial protein synthesis Kardaya, Dede; Wiryawan, K.G; Parakkasi, A; Winugroho, H.M
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 2 (2010)
Publisher : Indonesian Animal Sciences Society

Effect of slow-release urea on efficiency of rumen microbial protein synthesis (EMPS) was examined using an in vitro technique. The objective of this experiment was to reveal the in vitro slow-release urea characteristics of zinc-urea, zeolites-urea, and zeolites-zinc-urea in relation to EMPS observed in different incubation time. The experimental design employed was randomized block design with 4 x 3 factorial plus a control treatment, and conducted in two replications. Factors were various urea sources (urea, zinc-urea, zeolites-urea, and zeolites-zinc-urea) and molasses concentrations (0%, 6%, and 12%) in rice straw-based diets. The control treatment was the rice straw-based diets containing neither urea nor molasses. Diets consisting of 45% rice straw and 55% concentrates (DM basis) were formulated to have similar N and TDN levels. Responses of parameters measured were subjected to MANOVA using the GLM procedure of SPSS 16.00 and differences among mean values, if applicable, were examined using HSD-test. Orthogonal comparisons were used to determine the effects of the control treatment vs. various urea sources. Results indicated that treatment of UZ combined with 6% of molasses showed the highest microbial biomass production (2.71 mg/l) at 24 hours fermentation period with its peak production estimation (3.2 mg/l) reached at 33.5 hours of fermentation period. Moreover, UZ treatment resulted in the highest microbial protein synthesis (1,381.45 Â± 77.1 mg/l) at 24 hours fermentation period with its peak microbial protein synthesis estimation (1,756.04 mg/l) reached at 33.7 hours of fermentation period. The highest EMPS (25.98 Â± 1.21 mg/100 mg OMD) was achieved when ration contained 6% of molasses. Key words: Slow-Release Urea, Microbial Protein Synthesis, Microbial Biomass, In vitro

Pengaruh Tepung Cangkang Rajungan (Portunus pelagicus) dalam Ransum terhadap Kadar Kolesterol Serum dan Pertambahan Bobot Badan Tikus Putih (Rattus norvegicus) I U Warsono; M Fattah W; A Parakkasi
Media Peternakan Vol. 27 No. 2 (2004): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Crab shells contain the highest percentage of chitin. Chitin and its derivatives have many properties that make them attractive for a wide variety of applications. This study was conducted to determine the effect of crab (Portunus pelagicus) shells as a source of chitin in ration on serum cholesterol and weight gain of rats (Rattus norvegicus). The study was carried out in a factorial experiment 2 x 5 with 3 replications. The first factor is sex and the second factor is crab shells of 5 levels i.e., 0%, 5%, 10%, 15%, and 20% in ration. The study showed that there were no interaction effect between sex and crab shells levels in diet on all parameters. Serum cholesterol levels decreased (P > 0.05) and weigh gain increased (P < 0.01) when crab shells were included in the diets. The highest reduction of serum cholesterol level (14,8%) was observed at crab shells level of 10%, but the highest weight gain was reached at 5% inclusion of crab shells. These results suggest that crab shells in rat diets reduced serum cholesterol level and increased weight gain. Key words: crab shells, cholesterol, gain, rat

Pengaruh Level Protein, Vitamin A dan Vitamin E terhadap Pertambahan Bobot Badan dan Beberapa Fungsi Reproduksi Tikus Putih (Rattus norvegicus) . Yudi; A Parakkasi
Media Peternakan Vol. 28 No. 2 (2005): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Protein has the role in the development of physiological structure and body immunity system. Reproductive hormones, gametogenesis and fetus development are very much affected by the content of protein, vitamin A and E. Vitamin A is known to have a role in the integrity of epithel tissue, thus it has a great effect on the process of gametogenesis and embryogenesis. Vitamin E is recognized as an anti-sterility vitamin because of its important role as an intercellular antioxidant, in absorbing and storing vitamin A. This research was intended to determine the effect of protein content (15%, 18% and 21%), vitamin A (2000 IU/kg of feed), and vitamin E (30 mg/kg of feed) on body weight and some characteristics of reproduction of white mice (Rattus novergicus). The reproduction characteristics observed on female were the litter size and their body weight at birth, whereas those on male were fertility and morphology of spermatozoa. The research results showed that there were some effects of protein, vitamin A and E on increased body weight of female. The best result with a significant change (P

Pengaruh Suplemen Katalitik terhadap Karakteristik dan Populasi Mikroba Rumen Domba H T Uhi; A Parakkasi; B Haryanto
Media Peternakan Vol. 29 No. 1 (2006): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Dry season resulted in lower availability of ruminant feeds with subsequent effects on reduction of sheep productivity; therefore nutritive supplement may be required. The objective of this experiment was to study the effect of supplementation of catalytic substrate consisting of gelatinized sago, ammonium sulfate, Co and Zn on the sheep rumen characteristics and its microbial population. Forty lambs with an average live weight of 13 kg were divided into 8 blocks to test 5 feeding treatments. The treatments were feeding low quality forage without supplement (R1), R1 plus catalytic supplement at 10% of ration (R2), 20% (R3), 30% (R4) and a positive control treatment (R0 = R1 + soybean meal). Parameter measurements included rumen pH, ammonia, VFA and microbial population. It was observed that the rumen pH ranging from 6,06 (R1), 6,15 (R2), 6,45 (R4), 6,58 (R3) and 6,85 (R0). The rumen concentrations of ammonia were 5,83 mM (R3), 6,01 mM (R4), 6,35 mM (R2), 8,30 mM (R0) and 9,36 mM (R1) with total volatile fatty acid concentration ranging from 154, 88 mM (R1), 163,70 mM (R2), 180,89 mM (R0), 188,79 mM (R4) and 194,71 mM (R3). Population of rumen bacteri for R3 was 6,09 x 109 cell/ml, which was greater than RO (5,57 x 109 cell/ml), R1 (4,36 x 109 cell/ml), R2 (4,15 x 109 cell/ml), R4 (5,60 x 109 cell/ml), while protozoa R3 (2,59 x 106 cell/ml), was lower than RO (3,51 x 106 cell/ml) R1 (5,49 x 106 cell/ml) R2 (5,61 x 106 cell/ml) R4 (3,31 x 106 cell/ml). Catalytic supplement at 20% of ration (R3) resulted in a normal rumen concentration of ammonia and pH, and increased VFA concentration. It was concluded that catalytic supplement at 20% of ration was the appropriate level for optimal rumen characteristics.Key words : catalytic supplement, minerals, microbes, rumen, sheep

Efektivitas Daun Murbei Sebagai Pengganti Konsentrat dalam Sistem Rumen in Vitro S Syahrir; K G Wiryawan; A Parakkasi; M Winugroho; O.N P Sari
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Mulberry leave has a great potential as animal feed because of its high nutrient content. It also has deoxynojirimycin (DNJ) compound, that is potential to increase fermentability of fibrous feed in ruminal system. An in vitro experiment was conducted to investigate the capability of mulberry leaves to substitute concentrate as feed for ruminant in increasing fermentability of fibrous feed in ruminal system. This experiment was carried out using randomized block design with five treatments and four replications. The treatments were: P0 (50% rice straw + 50% concentrate) as a control, P1 (50% rice straw + 37.5% concentrate + 12.5% mulberry leave), P2 (50% rice straw + 25% concentrate + 25% mulberry leave), P3 (50% rice straw + 12.5% concentrate + 37.5% mulberry leave), P4 (50% rice straw + 50% mulberry leave). Variables measured were fermentability (NH3 and VFA concentrations), pH, gas production, dry matter and organic matter digestibility. Data were analyzed using analysis of variance and duncan multiple range test was further used to test the significant differences. VFA concentration, dry matter digestibility and organic matter digestibility were significantly difference (P < 0.05) among treatments. However, there was no significant effect on the other variables. It is concluded that mulberry leave are able to substitute the concentrate and increased fermentability of fibrous feed in ruminal system. Key words: mulberry leave, rice straw, ruminal fermentation, in vitro

Substitution of Concentrate with Mulberry Leaves in Ongole Grade Cattle Fed Rice Straw Based Diet S Syahrir; K G Wiryawan; A Parakkasi; M Winugroho; A Natsir
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

An experiment was conducted to assess the substitution of concentrate with mulberry (Morus spp.) leaves combined with rice straw offered to beef cattle. The study used 12 male Ongole grade cattle weighing 217+10.53 kg. Rations consisted of rice straw, mulberry leaves and concentrate. Research was carried out according to completely randomized design with 3 treatments and 4 replications for each treatment. The treatments were: P1= 50% rice straw + 50% concentrate; P2= 50% rice straw + 25% concentrate + 25% mulberry; P3= 50% rice straw + 50% mulberry. The results of the study indicated that rumen fermentation in cattlle fed on P2 ration was more effective than those receiving P1 and P3 rations. The fermentation was characterized by a higher production of total VFA and allantoin excretion in the urine. The presence of 1-deoxynojirimycin (DNJ) compound of mulberry leaves in the rumen system in treatment P2 and P3 improved digestibility of fiber fractions of rice straw-based rations. Mulberry leaves in rations also accounted for isoacids in the rumen system, which supported the development of cellulolytic bacteria. In conclusion, mulberry leaves containing concentrate had a potency to substitute concentrate when used together with fiber source feed such as rice straw.

The use of formaldehyde protected palm kernel meal and its effects on animal performance, nitrogen utilization and unsaturated fatty acid composition in Priangan sheep K.G Wiryawan; A Parakkasi; R Priyanto; I.P Nanda
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 4 (2007): DECEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An experiment was conducted to investigate the effect of formaldehyde-treated Palm Kernel Cake (BIS) in male Priangan sheep diet and its effect on animal performance, N utilization, and unsaturated fatty acids composition of musculus longisimus dorsal. The experiment was designed based on completly randomized design with four treatments and nine replications. The treatments consisted of four levels of formaldehyde treated BIS: R1 (0%), R2 (15%), R3 (30%) and R4 (45%). Results indicated that formaldehyde treated BIS up to 45% in diet did not negatively affect consumption, dry matter digestibility and daily live weight gain. Formaldehyde protection especially in R4 could significantly reduce ruminal N-NH3 concentration, increased protein digestibility and nitrogen retention, but did not influence allantoin urine concentration. Unsaturated fatty acid composition of musculus longisimus dorsal was not affected by formaldehyde treated BIS in diet. It is concluded that the inclusion of 45% formaldehyde treated BIS in diet did not negatively affect animal performance, and R4 is the most effective in improving nitrogen utilization in male Priangan sheep, but unable to alter the unsaturated fatty acids composition of musculus longisimus dorsal. Key Words: Palm Kernel Cake, Formaldehyde, Performance, Nitrogen, Unsaturated Fatty Acids

In vitro slow-release urea contained in rice straw-based diets to increase efficiency of rumen microbial protein synthesis Dede Kardaya; K.G Wiryawan; A Parakkasi; H.M Winugroho
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 2 (2010): JUNE 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Effect of slow-release urea on efficiency of rumen microbial protein synthesis (EMPS) was examined using an in vitro technique. The objective of this experiment was to reveal the in vitro slow-release urea characteristics of zinc-urea, zeolites-urea, and zeolites-zinc-urea in relation to EMPS observed in different incubation time. The experimental design employed was randomized block design with 4 x 3 factorial plus a control treatment, and conducted in two replications. Factors were various urea sources (urea, zinc-urea, zeolites-urea, and zeolites-zinc-urea) and molasses concentrations (0%, 6%, and 12%) in rice straw-based diets. The control treatment was the rice straw-based diets containing neither urea nor molasses. Diets consisting of 45% rice straw and 55% concentrates (DM basis) were formulated to have similar N and TDN levels. Responses of parameters measured were subjected to MANOVA using the GLM procedure of SPSS 16.00 and differences among mean values, if applicable, were examined using HSD-test. Orthogonal comparisons were used to determine the effects of the control treatment vs. various urea sources. Results indicated that treatment of UZ combined with 6% of molasses showed the highest microbial biomass production (2.71 mg/l) at 24 hours fermentation period with its peak production estimation (3.2 mg/l) reached at 33.5 hours of fermentation period. Moreover, UZ treatment resulted in the highest microbial protein synthesis (1,381.45 ± 77.1 mg/l) at 24 hours fermentation period with its peak microbial protein synthesis estimation (1,756.04 mg/l) reached at 33.7 hours of fermentation period. The highest EMPS (25.98 ± 1.21 mg/100 mg OMD) was achieved when ration contained 6% of molasses. Key words: Slow-Release Urea, Microbial Protein Synthesis, Microbial Biomass, In vitro

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