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Taufiq F. Pasiak
Universitas Sam Ratulangi
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology

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Journal : JURNAL BIOMEDIK

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Gambaran Mikrokopik Serebelum pada Hewan Coba Postmortem Nangoy, Belinda V.; Kalangi, Sonny J. R.; Pasiak, Taufiq F.
Jurnal Biomedik : JBM Vol 11, No 1 (2019): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.11.1.2019.23205

Abstract

Abstract: After death, there will be cellular changes that cause definite signs of death. These changes could be used to determine the time of death. This study was aimed to determine the microscopic changes of the cerebellum during 1 hour to 24 hours postmortem. This was a descriptive study. Four domestic pigs of more than 90 kg were used as animal models. After being killed, we made slices in the pig heads to expose and observe cerebellar microscopic changes in several time intervals, as follows: 90 minutes, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, 11 hours, 12 hours, 13 hours, 14 hours, 15 hours, 16 hours, 17 hours, 18 hours, 19 hours, 20 hours, 21 hours, 22 hours, 23 hours, and 24 hours postmortem. The results showed that the cerebellum became progressively pale and softened at 8 hours postmortem. Congestion in all tissues occured at 2 hours postmortem, however 69.2% of the Purkinje cells still had normal nuclei. At 7 hours postmortem, Purkinje cells began to enlarge associated with karyorrhexis, and at 21 hours postmortem most of the cells shrank. Albeit, at 24 hours postmortem the cerebellar layers could still be identified and some Purkinje cells with normal morphology could be found. Conclusion: Microscopic changes could be identified at 2 hours postmortem in the form of congestion of the cerebellar layers. Purkinje cells underwent karyorrhexis at 7 hours postmortem and shrank at 21 hours postmortem.Keywords: Purkinje cells, cerebellar layers, postmortemAbstrak: Setelah kematian, terjadi perubahan pada sel-sel yang menimbulkan tanda-tanda pasti kematian. Perubahan-perubahan yang terjadi dapat membantu menentukan saat kematian dalam suatu kasus hukum. Penelitian ini bertujuan untuk mengetahui perubahan mikroskopik serebelum selama interval waktu 1 jam hingga 24 jam postmortem. Jenis penelitian ialah deskriptif. pada hewan coba babi dengan rerata berat lebih dari 90 kg. Setelah hewan coba dimatikan, dibuat irisan di bagian kepala untuk menampakkan serebelum dan mengamati perubahan mikroskopiknya pada rentang waktu 90 menit, 2 jam, 3 jam, 4 jam, 5 jam, 6 jam, 7 jam, 8 jam, 9 jam, 10 jam, 11 jam, 12 jam, 13 jam, 14 jam, 15 jam, 16 jam, 17 jam, 18 jam, 19 jam, 20 jam, 21 jam, 22 jam, 23 jam, dan 24 jam postmortem. Hasil penelitian mendapatkan serebelum tampak pucat dan melunak secara progresif pada 8 jam postmortem. Kongesti di semua jaringan mulai terjadi pada 2 jam postmortem dan ditemukan 69,2% sel Purkinje berinti yang masih normal. Sel Purkinje mulai membesar dan inti mengalami karioreksis pada 7 jam postmortem tetapi pada 21 jam postmortem sel-sel tersebut tampak menyusut. Meskipun demikian hingga 24 jam postmortem struktur lapisan serebelum masih dapat diidentifikasi dan sel Purkinje dengan morfologi normal masih ditemukan. Simpulan: Perubahan mikroskopik serebelum sudah dapat diidentifikasi pada 2 jam postmortem yaitu berupa kongesti lapisan serebelum. Sel Purkinje mengalami karioreksis pada 7 jam postmortem dan menyusut pada 21 jam postmortem.Kata kunci: sel Purkinje, lapisan serebelum, postmortem
HUBUNGAN TINGGI BADAN DENGAN PANJANG KAKI PADA MAHASISWA FAKULTAS KEDOKTERAN UNSRAT Paluta, Reniwaty S.; Tanudjaja, George N.; Pasiak, Taufiq F.
Jurnal Biomedik : JBM Vol 5, No 1 (2013): JURNAL BIOMEDIK : JBM Suplemen
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.5.1.2013.2611

Abstract

Abstract: Identification of a dead body is important in determining the clarity of one’s identity. Height is an important parameter in the process of identification and is one of the fields of study of physical anthropology. It is expected that the height can be determined by using the measurements of long bones, such as metatarsal bones and phalanges. This study aimed to find out the relationship between the height and foot length in the students of Faculty of Medicine University of Sam Ratulangi, Manado. This was a descriptive analytic study with cross-sectional design. Samples were selected by using systematic sampling methods. As samples, we used 74 students (registered in 2010) comprising 37 males and 37 females. Data were analyzed with a Pearson correlation analysis as well as a simple linear regression analysis. The results showed that there was a strong correlation between height and foot length with the correlation coefficients (r) of 0.846 for all samples, 0.520 for male students, and 0.711 for female students. The simple linear regression analysis resulted in three formulas: male height = 112.930 + 2.361 × foot length; female height = 4.223 + 64.241 × foot length; and overall height = 4.717 + 54.729 × foot length. Conclusion: There was a strong relationship between the heights and the foot lengths of students at the Faculty of Medicine University of Sam Ratulangi University Manado. Keywords: identification, height, foot length.   Abstrak: Identifikasi ialah pemeriksaan penting dalam menentukan kejelasan identitas seseorang. Tinggi badan merupakan parameter penting dalam proses identifikasi dan bidang telaah antropologi ragawi. Tinggi badan diharapkan dapat ditentukan dengan menggunakan pengukuran tulang-tulang panjang, diantaranya tulang metatarsal dan falang. Penelitian ini bertujuan untuk mengetahui hubungan antara tinggi badan dengan panjang kaki pada mahasiswa Fakultas Kedokteran Universitas Sam Ratulangi Manado. Penelitian ini bersifat deskriptif analitik dengan rancangan cross-sectional. Sampel berjumlah 74 mahasiswa yang terdaftar pada tahun 2010, terdiri dari 37 laki-laki dan 37 perempuan. Sampel dipilih menggunakan cara systematic sampling. Data dianalisis dengan uji korelasi Pearson dan analisis regresi linier sederhana.  Hasl penelitian memperlihatkan terdapatnya hubungan kuat antara tinggi badan dan panjang kaki dengan koefisien korelasi (r) keseluruhan 0,846, pada laki-laki 0,520, dan pada perempuan 0,711. Dari hasil analisis regresi linier sederhana didapatkan rumus Tinggi Badan (TB) laki-laki = 112,930 + 2,361 × panjang kaki, TB perempuan = 64,241 + 4,223 × panjang kaki, dan secara keseluruhan TB = 54,729 + 4,717 × panjang kaki. Simpulan: Terdapat hubungan antara tinggi badan dan panjang kaki pada mahasiswa Fakultas Kedokteran Universitas Sam Ratulangi Manado. Kata kunci: identifikasi, tinggi badan, panjang kaki.
Co-Authors Arie, Febryola Channesya Sampetoding, Channesya Christanti Sambeka Djon Wongkar Fitria Angraini Dalili Friska G. Batoteng George N. Tanudjaja George Tanudjaja, George Juniarsih ., Juniarsih Kumesan, Okky Legoh, Christantio Liputra, Osvaldo T. Lomban, Arantsa Lumingkewas, Priscilla E. Maria A. Ch. Ticoalu Martha M. Kaseke Melkior T. Makawekes, Melkior T. Merry C. Warokka, Merry C. Nangoy, Belinda V. Pakaya, Putra Reniwaty S. Paluta Runtuwarow, Stardia Ruru, Pingkan M. Shane H. R. Ticoalu Shane H.R. Ticoalu Simanullang, Magdalena I. Sonny J. R. Kalangi Sonny J.R. Kalangi Sunny Wangko Supriadi . Togatorop, Desy