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UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN JERUK PURUT (Citrus hystrix (D.C)) TERHADAP BAKTERI Propionibacterium acnes Fitriyanti Fitriyanti; Muhammad Hafizudin; Muhammad Nazarudin
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 5 No 1 (2020): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.201 KB) | DOI: 10.36387/jiis.v5i1.376

Abstract

Lime leaves contain flavonoids, tannins, saponins and steroids which are thought to have antibacterial activity. This study aimed to determine the antibacterial activity of the methanol extract of Lime leaves to P.acnes bacteria and to determine the activity of methanol extract of the leaves of Lime based on the value of the diameter of the inhibitory zone and the inhibition category. Lime Leaves extracted by the maceration method using methanol solvent followed screening tests phytochemical. The antibacterial activity test was carried out by the sump method with 6 concentrations of methanol extract of the leaves of Lime according to 25mg/mL, 50mg/mL, 100mg/mL, 200mg/ mL, 300mg/mL and 400mg/mL, positive control of clindamycin 0,1% and negative control Na-CMC 0,5%. The results of phytochemical screening, methanol extract positively contain flavonoids, saponins, tannins, and steroids. Antibacterial test results showed that the inhibition zone of the Lime leaves of the methanol extract in the concentration of 25 mg/ml, and 50 mg/mL was 8.35 and 9.4 mm (medium category), concentrations of 100 mg/ml, and 200 mg/mL were 11.77, and 19.45 mm (strong category), and concentrations of 300mg / ml, and 400mg/mL, namely 21.05, and 22.87 mm (very strong categories). SPSS test results there were significant differences between treatment groups (sig <0.05).
Pengaruh Getaran Centrifuge Terhadap Hasil Pemeriksaan Laju Endap Darah (LED) Muhammad Nazarudin
Jurnal Labora Medika Vol 3, No 1 (2019): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.687 KB) | DOI: 10.26714/jlabmed.3.1.2019.10-14

Abstract

The Erythrocyte Sedimentation Rate (LED) is a hematological examination which is a preliminary examination in establishing a patient's diagnosis. Many LED tests have factors that can affect the results, one of them is vibration. In some health service units, LED checks are carried out close to the Centrifuge, which has vibrations, for reasons of room efficiency. This study aims to determine the effect of Centrifuge vibration on the results of the Westergren method LED examination. The study was conducted with 6 repetitions. This type of research used is quasi-experimental with static group comparison design. The research obtained is presented in table form, followed by ANOVA test. ANOVA test gives a significant value of 0.305 which indicates that there is no significant effect by the vibration of the Centrifuge on the LED results with variations in the distance of 0.5m, 1m, and 1.5m
UJI EFEKTIVITAS ANTIBAKTERI ESKTRAK ETIL ASETAT BAWANG DAYAK (Eleutherine palmifolia Merr) TERHADAP STAPHYLOCOCCUS AUREUS DENGAN METODE SUMURAN Fitriyanti Fitriyanti; Abdurrazaq Abdurrazaq; Muhammad Nazarudin
Jurnal Ilmiah Manuntung Vol 5 No 2 (2019): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v5i2.278

Abstract

Dayak onion (Eleutherine palmifolia Merr.) is a multifunctional medicinal plant for various diseases, one of which is an antibacterial. Dayak Onions (E. palmifolia Merr.) contains an alkaloid, flavonoid, triterpenoid, and saponin compound which has antibacterial properties. This study aims to determine the effect of the ethyl acetate extract of Dayak Onion tubers inhibits the growth of Staphylococcus aureus bacteria. Dayak onion tuber was extracted using ethyl acetate solvent by the maceration method until thick extract was obtained. The concentration extract used were 30 mg/ml;15 mg/ml;7,5 mg/ml;3,75 mg/ml; 1,875 mg/ml; and 0,9375 mg/ml tested for antibacterial effectivity against Staphylococcus aureus using Mueller Hinton Agar (MHA) with the wells method. The result showed that the ethyl acetate of Dayak onion with MEC (Minimum effective concentration) 3,75 mg/ml obtained inhibition zone of 10,367 mm while at the highest concentration of 30 mg/ml had the best antibacterial effectiveness. The effective concentration of ethyl acetate onion of Dayak tuber which is 30 mg/ml obtained inhibition zone of 18,404 mm even in the moderate category.