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All Journal Agrotech Journal
I. Indarwati
Wijaya Kusuma University Surabaya
Agriculture, Biological Sciences & Forestry

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In Vitro Culture of Dragon Fruit (Hylocereus polyrhizus): Callus and Anthocyanin Production I. Indarwati
Agrotech Journal Vol 6, No 2 (2021): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v6i2.1659

Abstract

Tissue culture biotechnology has been widely used to produce secondary metabolites. Anthocyanin as the secundary metabolic content in Dragon Fruits was thought to be able to liberate free radicals and have pharmacological activities as antioxidants and anti-aging. Anthocyanin can be produced in vitrothrough callus production by tissue culture. The study aims to produce callus cultures that have the potensial to produce secondary metabolites. This research was conducted in the tissue culture laboratory. The study used a completely randomized design method with a factorial pattern. Factor 1 : Type of explants ( M1): Explants from young shoots; (M2) Explants from dragon fruit callus. Factor II: The number of explant each culture tube. There are J1,J2, 3 and J4 with (1;2;3;4 explants in each culture tube. Murashige and Skoog + 15 % sucrose were used as media culture. The results of study showed that: callus began to form at 7 weeks after planting. (1) MS Media with the addition of 15 % sucrose had a significant effect on increasing the anthocyanin content in the callus of Dragon fruit formed. (2) Callus formed from Shoot Dragon fruit contain Anthocyanin 0,74 % ; (3) The Callus formed from Shoot and callus Dragon Fruits contain secondary martabolites of Anthocyanins ranging from 0,68%–0,76%. The highest Anthocyanin content (0,76%) was produced in treatmant J3 ( 3 Explant each culture tube)
The Impact of Road Light on Growth and Result of Soybean Plant Jajuk Herawati; I. Indarwati; Tatuk Tojibatus S.; Mochamad Thohiron; Heru Prasetyo
Agrotech Journal Vol 5, No 2 (2020): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v5i2.1399

Abstract

Until now, soybean is still one of the priority food commodities in Indonesia. in the agricultural revitalization program launched by the government in 2005, due to the high price volatility that did not rule out the possibility of shaking the Indonesian economy. Soybean plants can provide positive and negative responses to environmental changes growing above and in the soil.  This response can be known from phenotypic and physiological changes in plants. The environment on land which influences the growth of soybean plants mainly is the duration and intensity of irradiation, air temperature, CO2 content in the atmosphere. The study aims to determine the impact of street lighting on the growth and yield of soybean plants.  The study used a Randomized Block Design Method with 3 treatments,  and each treatment was repeated 9 times, so that it takes 27 treatment plots. J0= Distance of street lighting to soybean land (meters), J1: 50 meters, J2: 60 meters, and J3: 70 meters.  Observations were made one week after planting at 7-day intervals for growth parameters (plant height and number of leaves), while for the production parameters (Number of Content Pods/Plants, Dry Weight (DW) 100 Seeds, DW Seeds/Plots, and DW Seeds/Ha) are carried out after harvest. From the results of the study it can be concluded that there are real differences in the parameters of growth and production (number of filled pods, DW 100 seeds, DW seeds/plot, and DW seeds/ha), where the J3 treatment is capable of producing 2.89 tons/ha (an increase in dry weight of seeds/ha by 28.4%), compared to J1 2.24 tonnes/ha , although not significantly different from Treatment  J2
Co-Authors Heru Prasetyo Jajuk Herawati Mochamad Thohiron Tatuk Tojibatus S.