Previous research on biodiesel properties, based on the 2015 Indonesian National Standard (SNI), limited its characteristics to testing. Therefore, the author conducted further research on the performance and durability of single-cylinder, four-stroke diesel engine components. This led to the future use of B100 biodiesel as an alternative fuel if commercially produced. The fuel used is B100 biodiesel and biodiesel plus G57 additive. G57 additive or D.04.BXD catalyst, which can achieve low emissions, high quality, high fuel performance, and high efficiency (PT. SEGARA, 2022). The results of the 200-hour durability test revealed no changes in wear related to piston gap and groove size, or the visual condition of the main bearing. The performance results obtained by Biodiesel B100 obtained an average engine power of 0.99 Hp, an average torque of 3.5 nm, SFOC by producing an average of 798.47 Gr / kWh better than Biodiesel B100 + G57 at high idle speed the engine power obtained was an average of 0.97 Hp, torque with an average of 3.4 nm, SFOC obtained an average of 817.34 Gr / kWh, at max torque speed Biodiesel B100 + G57 obtained an average engine power of 2.93 Hp, torque with an average of 13.03 nm, SFOC produced an average of 392.08 Gr / kWh better than biodiesel B100 which obtained an average engine power of 2.84 Hp, an average torque value of 12.56 nm, The average SFOC value is 473.04 Gr/kWH and B100+G57 biodiesel at rated rpm speed obtained an average engine power of 4.6 Hp, an average torque of 15.08 nm, an average SFOC of 375.40 Gr/kWH much better than B100 biodiesel which obtained an average engine power of 4.1 Hp, an average torque of 13.57 nm, an average SFOC of 451.54 Gr/kWH. Lab test results of B100+G57 biodiesel content have a flash point of 164 °C compared to B100 which obtained a greater result of 168 °C. The water content in B100 biodiesel is 0.118% smaller than the water content in B100+addictive G57 biodiesel of 0.127%. The density of B100 biodiesel is 0.880 g/cm3, slightly higher than that of B100+G57 biodiesel, which is 0.879 g/cm3. The viscosity of B100 biodiesel is 8.05 cSt, while B100+G57 biodiesel has a viscosity of 7.67 cSt.