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A. Pertiwiningrum
Faculty of Animal Science, Gadjah Mada University, Jl. Fauna 3 Bulaksumur Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Pengaruh Umur Potong dan Konsentrasi Larutan Asam Asetat terhadap Sifat Fisik dan Kimia Gelatin Kulit Babi Sompie, M.; Triatmojo, S.; Pertiwiningrum, A.; Pranoto, Y.
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 10, No 1 (2012): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.v10i1.4816

Abstract

This research was aimed to study the influence of animal age and acetic acid solution concentration on physical and chemical properties. The experiment used Randomized Complete Design (RCD) with two factors and three replicates. The first factor was animal age consisted 3 levels (5, 7 and 9 months). The second factor was concentration of acetic acid solution consisted of 3 levels (2, 4 and 6 percents). The result showed that interaction of animal age period and concentration of acetic acid had no significant effect on gel strength, moisture content, ash content and fat content of pig skin gelatin. It was concluded that pigskin gelatin from ages of 5, 7 and 9 months and concentration acetic acid 2, 4 and 6 % had similar physical and chemical peoperties with the commercial gelatin.
THE EFFECTS OF ANIMAL AGE AND ACETIC ACID CONCENTRATION ON PIGSKIN GELATIN CHARACTERISTICS Sompie, M.; Triatmojo, S.; Pertiwiningrum, A.; Pranoto, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 3 (2012): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.3.176-182

Abstract

This research was aimed to study the influence of animal age and concentration of the acetic acidsolution on physical and chemical properties of pigskin gelatin. The experiment used CompletelyRandomized Design (CRD) with two factors. The first factor was animal age consisted of 3 levels (5, 7and 9 months). The second factor was concentration of acetic acid solution consisted of 3 levels (2, 4and 6 percents). The result showed that animal age had significant effect (P<0.01) on the yields, gelstrength, viscosity, protein content and pH value but interaction of animal age and concentration ofacetic acid had no significant effect (P>0.05) on the yields, gel strength, viscosity, protein content andpH value. It was concluded that pigskin gelatin from ages of 5, 7 and 9 months and acetic acidconcentration of 2, 4 and 6% had similar characteristics to the commercial gelatin, but the optimumproduction of gelatin was combination of pigskin gelatin from 7 months and of 2% acetic acid.
Isolation and identification of bacterial protease enzyme of leather waste Pertiwiningrum, A.; Anggraini, F. D.; Fitrianto, N. A.; Rochijan, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 1 (2017): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.1.33-41

Abstract

The objectives of this study were to isolate and identify bacteria which produced protease enzyme from liquid and solid waste of tannery, and their characterization of enzymatic activities. The bacterial isolation used a sample of liquid and solid waste from leather waste which taken from a different waste reservoirs (three liquid waste and one solid waste in unhairing phase). Data of the isolated bacteria, OD 600 nm bacterial growth, the identification of bacteria, and enzyme precipitation with 60% ammonium sulfate were analyzed descriptively. The colony diameter, diameter of clear zone, proteolytic index, and enzymatic activities characterization on difference of pH and temperature were analyzed using Completely Randomized Design, followed by Duncan’s New Multiple Range Test. The second sample from four samples of the isolated bacteria was tested further for their proteolytic activity. The morphology of the colony was circle, white, flat ledges and convex elevation, the basal cell morphology was red, gram-negative, non-motile, catalase positive and gelatin negative. The highest activity of enzyme on pH 11 with activity unit enzyme 45,18±1,77 U/ml and specific enzyme activity 43,19±1,69 U/mg and temperature of 40°C activity unit enzyme 54,02±1,89 U/ml and specific enzyme activity 51,65±1,8 U/mg. The activity of enzyme from protease were precipitated ammonium sulfate 60% showed a higher result of (75,8 U/ml) rather than rough protease.
Co-Authors Anggraini, F. D. Fitrianto, N. A. M. Sompie Rochijan Rochijan S. Triatmojo Y. Pranoto