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M. Poeloengan
Indonesia Research Center for Veterinary Science (IRCVS), Jl. R. E. Martadinata No. 30 P. O. Box 151, Bogor. West Java
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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THE EFFECT OF CLOVE STEM OIL (Oleum caryophylli) ON THE GROWTH OF ESCHERICHIA COLI ISOLATED FROM NATIVE CHICKEN, CATTLE AND PIG Poeloengan, M.; Noor, S.M.
Journal of the Indonesian Tropical Animal Agriculture Vol 34, No 4 (2009): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.34.4.279-283

Abstract

The objective of this study was to examine the effects of Clove Stem Oil (Oleum caryophylli) orCSO extracts on the growth of Escherichia coli. A 4 x 3 Factorial Design was applied in this study. Thefirst factor was 4 types of E. coli isolates that collected from native chicken (C), young cattle (A), piglet(B1) and pig (B2), while the second factor was 3 concentrations of CSO extract, that was 50.00, 25.00and 12.50 percents. Fifteen micro liters of CSO extract was dropped in sterile paper disks. These diskwere laid on the MEU agar media previously inoculated with each of the four isolates and wereincubated overnight at 370C. The bacterial growth inhibition zones were observed and measured. Theresults demonstrated that the higher concentration of the CSO extracts, the higher bacterial growthinhibition effects obtained. Fhe growth inhibition effects of the CSO extracts on E. coli isolates C, A, B1and B2 were significantly different at P<0.05. It could be concluded that the CSO extracts were effectiveto control the E. coli growth.
SCREENING FOR ANTIBACTERIAL PROPERTIES OF THREE MEDICINAL PLANTS AGAINST SALMONELLA SP. ISOLATES OBTAINED FROM BROILER CARCASS IN INDONESIA Poeloengan, M.; Andriani, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 1 (2013): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.1.34-40

Abstract

Antibacterial properties of three medicinal plants used widely amongst the native Indonesians, i.e.,sirih (Piper betel), garlic (Allium sativum) and jinten hitam (Nigella sativa) were screened and evaluatedagainst Salmonella sp. isolates. For this purpose, the three plants’ powder, aqueous and ethanol extractswere prepared. The tests’ results demonstrated the three plants’ anti Salmonella sp. activities. In thisconjunction, the aqueous extract of Allium sativum, the ethanol extracts of Nigella sativa, and Piperbetel, consecutively produced 17 mm, 15 mm and 13 mm diameter of bacterial growth inhibition zones.As a comparison, sensitivity tests of three commercial antibiotics, i.e., chloramphenicol, tetracycline,and gentamycin on Salmonella sp. isolates produced 19.7-27.3 mm growth inhibition zones. Garlichaving antimicrobial potential was comparable to the commercial antibiotics, can be used as adecontaminant against Salmonella sp. to maintain the quality of the broiler carcasses and thereforeprolonging the carcass shelf-life.
Co-Authors A. Andriani S.M. Noor