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Sudarmadi Poernomo
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Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP) Makful, Makful; Poernomo, Sudarmadi; Sunyoto, Sunyoto
Jurnal Hortikultura Vol 20, No 4 (2010): Desember 2010
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK. Penelitian dilaksanakan dari bulan Maret 2004 sampai dengan Desember 2005 di Laboratorium PemuliaanBalai Penelitian Tanaman Buah dan Laboratorium Bioteknologi Balai Penelitian Tanaman Perkebunan. Penelitianbertujuan mengetahui keragaman genetik manggis berdasarkan analisis molekuler dengan teknik AFLP menggunakanlima pasang primer. Analisis keragaman menggunakan program NTSys. Hasil amplifikasi amplified fragment lengthplymorphism (AFLP) terhadap sembilan sampel genom manggis menunjukkan adanya keragaman yang tinggi. Denganmetode unweighted pair-group with arithme average (UPGMA) pada koefisien jarak genetik 60% menghasilkansatu kelompok genom. Pada nilai koefisien kesamaan genetik 70%, aksesi manggis dapat dikategorikan menjadi tigakelompok yaitu kelompok 1 terdiri atas sampel 8-(Garcinia sp. manggis hutan-1), 13-(G. mundar), 17-(Garciniasp. manggis hutan-asam), kelompok 2 mencakup sampel 19-(G. mangostana Pasarminggu-2), 20-( G. mangostanaPasarminggu-1), 22-(G. mangostana Jayanti-2), dan kelompok 3 terdiri atas sampel 25-(G. malaccensis-Jambi),26-(G. malaccensis Bukit Kawang Medan PK 1), dan 27-(G. malaccensis Bukit Lang PK 2). Informasi variabilitasgenetik diharapkan dapat mendukung program pemuliaan manggis.ABSTRACT. Makful, S. Purnomo, and Sunyoto. 2010. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. This experiment was conducted fromMarch 2004 to December 2005 at the Laboratory of Plant Breeding of Indonesian Fruit Research Institute andthe Laboratory of Biotechnology of Indonesian Estate Crop Research Institute. The objective of the study was todetermine genetic diversity of mangosteen. The method used was AFLP technique with five pairs of primers, whiledata obtained was analyzed by the NTSys program. From the AFLP amplification of nine DNA samples, it wasproven that the accessions of mangosteen had a high degree of diversity. Based on analysis of AFLP and unweightedpair-group with arithme average (UPGMA) it was shown that the samples of mangosteen could be grouped into onecluster at relative ecludian distance of 60% and into three clusters at relative ecludian distance of 70%, i.e. cluster1for sample 8-( Garcinia sp. manggis hutan-1), 13-(G. mundar), 17-( Garcinia sp. manggis hutan-asam) samples,cluster 2 for sample 19-(G. mangostana Pasarminggu-2), 20-(G. mangostana Pasarminggu-1), 22-(G. mangostanaJayanti-2) samples, and cluster 3 for sample 25-(G. malaccensis-Jambi), 26-(G. malaccensis Bukit Kawang MedanPK 1), and 27-(G. malaccensis Bukit Lang PK 2) samples. Information of genetic variability is expected to supportthe mangosteen breeding program.
Formula Media Kultur Endosperm Jeruk Hasil Persilangan Antarklon Siem dengan Keprok dan Jeruk Besar Sunyoto, Sunyoto; Poernomo, Sudarmadi; Makful, Makful
Jurnal Hortikultura Vol 20, No 4 (2010): Desember 2010
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK. Pembentukan hibrida triploid renyah tanpa biji pada tanaman jeruk tipe keprok dapat dilakukan melaluikultur endosperm. Komposisi media yang tepat pada setiap tahapan kultur endosperm sangat menentukan keberhasilanpembentukan hibrida tersebut. Komposisi media induksi kalus sampai regenerasi jaringan endosperm jeruk belumbanyak diketahui. Penelitian bertujuan memperoleh formula media in vitro terbaik untuk pertumbuhan dan regenerasikalus endosperm jeruk hasil persilangan antarklon siem dengan keprok dan jeruk besar. Tanaman yang dihasilkandari kultur tersebut diharapkan menjadi kandidat varietas jeruk unggul baru yang mempunyai sifat buah tanpa bijidan berdaging buah renyah. Percobaan dilakukan di Laboratorium Kultur Biak Pemuliaan dan Plasma Nutfah BalaiPenelitian Tanaman Buah Tropika Solok. Penelitian menggunakan analisis diskriptif yang terdiri atas dua tahapkegiatan yang dilakukan mulai bulan Januari 2005 sampai dengan Januari 2006. Kegiatan pertama ialah produksikalus menggunakan media dasar MurashigeTungker (MT) dengan tiga formula. Kegiatan kedua yaitu regenerasikalus menggunakan media dasar MT dan Murashige Skoog (MS) dengan enam formula media. Hasil percobaanmenunjukkan bahwa media M3 = MT + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH + 0,5 ppm KT + 500 ppm ME,merupakan formula media inisiasi kalus yang terbaik. Formula media tersebut mampu menginisiasi terbentuknya kaluslebih cepat, menginduksi kalus yang lebih banyak, kekompakan struktur kalus baik, dan berwarna hijau. Formulamedia regenerasi yang terbaik ialah R3 = MT + 0,25 ppm BAP + 2 ppm GA3 + 500 ppm CH + 40 ppm Ads. Mediatersebut mampu mendukung terbentuknya tunas lebih panjang, jumlah daun, jumlah tunas, dan peningkatan jumlahakar dibandingkan persilangan lainnya. Kalus endosperm hasil persilangan intervarietas siem x keprok Dancy, dansiem x keprok Cina Konde merupakan kalus terbaik untuk diregenerasikan.ABSTRACT. Sunyoto, S. Purnomo, and Makful. 2010. Media Formula for Citrus Endosperm Culture ofHybridization between Clones of Tangerine and Clones of Mandarin and Pummelo. Formation of triploid hybridsof crunchy seedless mandarin types of citrus can be established through the induction of endosperm culture. Thecomposition of appropriate media at each stage of endosperm culture determines the success of teh hybrid production.The media compositions of callus of endosperm induction and its regeneration have not been known yet so far. Theaim of this research was to determine the best formula of in vitro medium to induce and regenerate endosperm callusof citrus hybrids. Plantlets produced from the culture were expected to be new superior varieties bearing seedlessand crispy fruits. Research was carried out in the Tissue Culture Breeding and Germplasm Laboratory, IndonesianTropical Fruits Research Institute from January 2005 to January 2006. Discriptive analysis was used in this research.The research composed of consecutive activities.The first activity was to produce callus using basic medium MT(MurashigeTungker) with three medium composition treatments, and the second one, was to regenerate embryoidcallus using MT and MS medium with six medium compositions. The results showed that medium of M3 = MT +5 ppm BAP + 2 ppm 2.4-D + 500 ppm CH + 0.5 ppm KT + 500 ppm ME was the best formula for callus initiation.This medium initiated faster growth of callus and gave higher percentage of callus formation that was more compactin structure, and green in color, than other tested media. The best medium for regeneration of embryoid callus wasR3 = MT + 0.25 ppm BAP + 2 ppm GA3 + 500 ppm CH + 40 ppm Ads. This medium increased the length of shoots,the number of leaves, shoots, and roots more than the other media. Endosperm calli produced from hybridizationintervarieties of tangerine x mandarin Dancy and tangerine x mandarin Cina Konde were the best calli for regeneration
Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP) Makful, Makful; Poernomo, Sudarmadi; Sunyoto, Sunyoto
Jurnal Hortikultura Vol 20, No 4 (2010): Desember 2010
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v20n4.2010.p%p

Abstract

ABSTRAK. Penelitian dilaksanakan dari bulan Maret 2004 sampai dengan Desember 2005 di Laboratorium PemuliaanBalai Penelitian Tanaman Buah dan Laboratorium Bioteknologi Balai Penelitian Tanaman Perkebunan. Penelitianbertujuan mengetahui keragaman genetik manggis berdasarkan analisis molekuler dengan teknik AFLP menggunakanlima pasang primer. Analisis keragaman menggunakan program NTSys. Hasil amplifikasi amplified fragment lengthplymorphism (AFLP) terhadap sembilan sampel genom manggis menunjukkan adanya keragaman yang tinggi. Denganmetode unweighted pair-group with arithme average (UPGMA) pada koefisien jarak genetik 60% menghasilkansatu kelompok genom. Pada nilai koefisien kesamaan genetik 70%, aksesi manggis dapat dikategorikan menjadi tigakelompok yaitu kelompok 1 terdiri atas sampel 8-(Garcinia sp. manggis hutan-1), 13-(G. mundar), 17-(Garciniasp. manggis hutan-asam), kelompok 2 mencakup sampel 19-(G. mangostana Pasarminggu-2), 20-( G. mangostanaPasarminggu-1), 22-(G. mangostana Jayanti-2), dan kelompok 3 terdiri atas sampel 25-(G. malaccensis-Jambi),26-(G. malaccensis Bukit Kawang Medan PK 1), dan 27-(G. malaccensis Bukit Lang PK 2). Informasi variabilitasgenetik diharapkan dapat mendukung program pemuliaan manggis.ABSTRACT. Makful, S. Purnomo, and Sunyoto. 2010. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. This experiment was conducted fromMarch 2004 to December 2005 at the Laboratory of Plant Breeding of Indonesian Fruit Research Institute andthe Laboratory of Biotechnology of Indonesian Estate Crop Research Institute. The objective of the study was todetermine genetic diversity of mangosteen. The method used was AFLP technique with five pairs of primers, whiledata obtained was analyzed by the NTSys program. From the AFLP amplification of nine DNA samples, it wasproven that the accessions of mangosteen had a high degree of diversity. Based on analysis of AFLP and unweightedpair-group with arithme average (UPGMA) it was shown that the samples of mangosteen could be grouped into onecluster at relative ecludian distance of 60% and into three clusters at relative ecludian distance of 70%, i.e. cluster1for sample 8-( Garcinia sp. manggis hutan-1), 13-(G. mundar), 17-( Garcinia sp. manggis hutan-asam) samples,cluster 2 for sample 19-(G. mangostana Pasarminggu-2), 20-(G. mangostana Pasarminggu-1), 22-(G. mangostanaJayanti-2) samples, and cluster 3 for sample 25-(G. malaccensis-Jambi), 26-(G. malaccensis Bukit Kawang MedanPK 1), and 27-(G. malaccensis Bukit Lang PK 2) samples. Information of genetic variability is expected to supportthe mangosteen breeding program.
Formula Media Kultur Endosperm Jeruk Hasil Persilangan Antarklon Siem dengan Keprok dan Jeruk Besar Sunyoto Sunyoto; Sudarmadi Poernomo; Makful Makful
Jurnal Hortikultura Vol 20, No 4 (2010): Desember 2010
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v20n4.2010.p%p

Abstract

ABSTRAK. Pembentukan hibrida triploid renyah tanpa biji pada tanaman jeruk tipe keprok dapat dilakukan melaluikultur endosperm. Komposisi media yang tepat pada setiap tahapan kultur endosperm sangat menentukan keberhasilanpembentukan hibrida tersebut. Komposisi media induksi kalus sampai regenerasi jaringan endosperm jeruk belumbanyak diketahui. Penelitian bertujuan memperoleh formula media in vitro terbaik untuk pertumbuhan dan regenerasikalus endosperm jeruk hasil persilangan antarklon siem dengan keprok dan jeruk besar. Tanaman yang dihasilkandari kultur tersebut diharapkan menjadi kandidat varietas jeruk unggul baru yang mempunyai sifat buah tanpa bijidan berdaging buah renyah. Percobaan dilakukan di Laboratorium Kultur Biak Pemuliaan dan Plasma Nutfah BalaiPenelitian Tanaman Buah Tropika Solok. Penelitian menggunakan analisis diskriptif yang terdiri atas dua tahapkegiatan yang dilakukan mulai bulan Januari 2005 sampai dengan Januari 2006. Kegiatan pertama ialah produksikalus menggunakan media dasar MurashigeTungker (MT) dengan tiga formula. Kegiatan kedua yaitu regenerasikalus menggunakan media dasar MT dan Murashige Skoog (MS) dengan enam formula media. Hasil percobaanmenunjukkan bahwa media M3 = MT + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH + 0,5 ppm KT + 500 ppm ME,merupakan formula media inisiasi kalus yang terbaik. Formula media tersebut mampu menginisiasi terbentuknya kaluslebih cepat, menginduksi kalus yang lebih banyak, kekompakan struktur kalus baik, dan berwarna hijau. Formulamedia regenerasi yang terbaik ialah R3 = MT + 0,25 ppm BAP + 2 ppm GA3 + 500 ppm CH + 40 ppm Ads. Mediatersebut mampu mendukung terbentuknya tunas lebih panjang, jumlah daun, jumlah tunas, dan peningkatan jumlahakar dibandingkan persilangan lainnya. Kalus endosperm hasil persilangan intervarietas siem x keprok Dancy, dansiem x keprok Cina Konde merupakan kalus terbaik untuk diregenerasikan.ABSTRACT. Sunyoto, S. Purnomo, and Makful. 2010. Media Formula for Citrus Endosperm Culture ofHybridization between Clones of Tangerine and Clones of Mandarin and Pummelo. Formation of triploid hybridsof crunchy seedless mandarin types of citrus can be established through the induction of endosperm culture. Thecomposition of appropriate media at each stage of endosperm culture determines the success of teh hybrid production.The media compositions of callus of endosperm induction and its regeneration have not been known yet so far. Theaim of this research was to determine the best formula of in vitro medium to induce and regenerate endosperm callusof citrus hybrids. Plantlets produced from the culture were expected to be new superior varieties bearing seedlessand crispy fruits. Research was carried out in the Tissue Culture Breeding and Germplasm Laboratory, IndonesianTropical Fruits Research Institute from January 2005 to January 2006. Discriptive analysis was used in this research.The research composed of consecutive activities.The first activity was to produce callus using basic medium MT(MurashigeTungker) with three medium composition treatments, and the second one, was to regenerate embryoidcallus using MT and MS medium with six medium compositions. The results showed that medium of M3 = MT +5 ppm BAP + 2 ppm 2.4-D + 500 ppm CH + 0.5 ppm KT + 500 ppm ME was the best formula for callus initiation.This medium initiated faster growth of callus and gave higher percentage of callus formation that was more compactin structure, and green in color, than other tested media. The best medium for regeneration of embryoid callus wasR3 = MT + 0.25 ppm BAP + 2 ppm GA3 + 500 ppm CH + 40 ppm Ads. This medium increased the length of shoots,the number of leaves, shoots, and roots more than the other media. Endosperm calli produced from hybridizationintervarieties of tangerine x mandarin Dancy and tangerine x mandarin Cina Konde were the best calli for regeneration
Co-Authors Makful Makful Makful Makful Sunyoto Sunyoto Sunyoto Sunyoto