Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Indonesian Journal of Biotechnology Jurnal Kesehatan Prima
Pramudji Hastuti
Department ofr Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Nursing Public Health

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Production and Optimization of Oleic Acid Ethyl Ester Synthesis Using Lipase From Rice Bran (Oryza sativa L.) and Germinated Jatropha Seeds (Jatropha curcas L.) by Response Surface Methodology Indro Prastowo; Chusnul Hidayat; Pramudji Hastuti
Indonesian Journal of Biotechnology Vol 17, No 1 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.424 KB) | DOI: 10.22146/ijbiotech.7848

Abstract

Recently, the fatty acid ethyl ester has been synthesized in place of fatty acid methyl ester since ethanol has been more renewable. In this research, oleic acid ethyl ester (OAEE) was synthesized using germinated jatropha seeds (Jatropha curcas.L) and rice bran (Oryza sativa) as source of lipase. The objective of the research was to optimize the synthesis conditions using Response Surface Methodology. Factors, such as crude enzyme concentration, molar ratio of oleic acid to ethanol, and the reaction time, were evaluated. The results show that lipase from germinated jatropha seeds had the hydrolitic and esterifi cation activity about 6.73 U/g and 298.07 U/g, respectively. Lipase from rice bran had the hydrolitic and esterifi cation activity about 10.57 U/g and 324.03 U/g, respectively. The optimum conditions of esterifi cation reaction using germinated jatropha seed lipase as biocatalyst were crude enzyme concentration of 0.31 g/ml, molar ratio of oleic acid to ethanol of 1 : 1.81, and reaction time of 50.9 min. The optimum conditions of esterifi cation reaction using rice bran lipase were crude enzyme concentration of 0.29 g/ml, molar ratio of oleic acid to ethanol of 1 : 2.05, and reaction time of 58.61 min. The obtained amounts of OAEE were 810.77 μmole and 626.92 μmole for lipases from rice bran and germinated jatropha seed, respectively.
THE CORRELATION NITRATE LEVELS AND NITRIC OXIDE (NO) IN BLOOD AS A RISKY FACTOR OF TYPE 2 DIABETES MELLITUS WITH HYPERTENSION AT RSUP DR. SARDJITO OF YOGYAKARTA Fransisca Shinta Maharini; Ahmad Hamim Sadewa; Pramudji Hastuti
Jurnal Kesehatan Prima Vol 12, No 1 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v12i1.97

Abstract

Abstract: Diabetes Mellitus (DM) is a metabolic disease characterized by hyperglycemia caused by insulin secretion disorder, insulin function or both of them. Hypertension is a disease identified by the increasing of diastolic blood pressure ≥90 mmHg or systolic blood pressure ≥140 mmHg. Diabetes mellitus and hypertension are two closely related condition and both are health problems which require accurate treatment, since it may accelerate complication of DM and hypertension. The occurrence of  DM and hypertension is higher than solely DM. DM causes hyperglycemia that triggers oxidative stress, increasing NO degradation leads to hypertension.This research utilized a case-control study involving with type 2 DM with hypertension patients as the case (n = 40) and type 2 DM without hypertension as the control groups (n = 40). The level of NO was analyzed using spectrophotometry. Correlation regression was used to analyzed the correlation between NO and blood pressure. A p value ˂ 0,05 was considered as significantly differance. The results showed mean level of NO was lower on subjects type 2 DM with hypertension compared to that of on subjects with type 2 DM without hypertension (p=0,023). There was a negative and week correlation between the level of NO and blood pressure (Systolic r = -0.233, p=0.037 and Diastolic r = -0.149, p=0.188).Keywords: Type 2 DM; Hypertension; Nitric Oxide.
Co-Authors Ahmad Hamim Sadewa Chusnul Hidayat Fransisca Shinta Maharini Indro Prastowo