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All Journal Jurnal Ilmu Pertanian Indonesia Golden Ratio of Human Resource Management
Dewi Praptiwi
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Agriculture, Biological Sciences & Forestry Social Sciences

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Journal : Jurnal Ilmu Pertanian Indonesia

 1 
Transfer Gen Badh2 Termutasi Varietas Aromatik Mentik Wangi ke Varietas Nonaromatik Ciherang Djarot Sasongko Hami Seno; Bambang Padmadi; Dewi Praptiwi; . Sugihartati; . Taufiq; Muhammad Taufan Fatahajudin; Helmy Ramadhan Al Anshary; Tri Joko Santoso; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Replacement of non-fragrant-native badh2 gene with mutated badh2 of fragrant rice is an alternative to engineer new fragrant rice varieties with good agronomic traits as those of non-fragrant. Fragrant gene (mutated badh2) of Mentik wangi donor was introgressed into non-fragrant Ciherang host through site-directed crossing. Mentik Wangi was crossed with Ciherang, and the progeny was further backcross until BC3F1. Bradbury marka-assisted PCR was used to select progeny in every cross and backcross generation. Ciherang, Mentik Wangi, and their cross/backcross showed different PCR profiles. The statues of badh2 gene (native/mutated), as well as alleles (homozygote/heterozygote) between samples were identified. Mutated badh2-introgression was also observed within the selected heterozygote cross or backcross progenies (Fl, BCl, BC2, and BC3), indicated successful transfer of mutated badh2 gene from donor to host.
Aplikasi Berbagai Marka Aromatik pada Varietas Padi Indonesia Djarot Sasongko Hami Seno; Satya Nugroho; Tri Joko Santoso; Dimas Adrianto; Dewi Praptiwi; Aniversari Apriana; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

This research applied various badh2.7 and badh2.2 fragrant markers (Bradbury et al., 2005b, Lang and Buu 2008, Shi et al., 2008, Sakthivel et al., 2009) on popular Indonesia non-fragrant (Ciherang, Fatmawati) and fragrant (Pandan wangi, Rojo Lele, Mentik Wangi, Gunung Perak, Pulu mandoti, Pare Kembang, Sintanur) rice varieties. For comparison, IR64, Nippon bare and Taipei 309 were included. Rice DNA samples were isolated from young  leaves, and PCR amplified using each of those fragrant markers. Results using all badh2.7 markers were consistently supported the existence of 2 group badh2.7 mutation pattern, while the use of badh2.2 marker indicated that there was no ex on 2 mutation. Badh2.7 sequence analysis of non-fragrant Ciherang, and aromatik member of group 1 (Pandan Wangi), as well as group 2 (Mentik Wangi) showed different mutation pattern. 
Co-Authors . Sugihartati . Taufiq Aniversari Apriana Bambang Padmadi Cedaryana, C. Dimas Adrianto Djarot Sasongko Hami Seno Helmy Ramadhan Al Anshary Muhammad Taufan Fatahajudin Sari, Rindi Nurlaila Satya Nugroho Tri Joko Santoso Zainal Alim Mas'ud