Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Paediatrica Indonesiana
AJP Veerman
Unknown Affiliation
Health Professions Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
High frequency of the 3R/3R polymorphism in the thymidylate synthase enhancer region in Indonesian childhood acute lymphoblastic leukemia IDG Ugrasena; Sutaryo Sutaryo; Edy Supriadi; Laura Vroling; Jacqueline Cloos; Jan Hendrik Hooijberg; AJP Veerman
Paediatrica Indonesiana Vol 46 No 3 (2006): May 2006
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/pi46.3.2006.103-12

Abstract

Background Deoxyuridylate monophosphate (dTMP) is neces-sary for DNA synthesis and thymidylate synthase (TS) is an im-portant target of cancer chemotherapy. Ethnic variations of thepolymorphic tandem repeat sequence in the enhancer region ofthe TS promoter has previously been described to influence theoutcome of acute lymphoblastic leukemia (ALL). A triple repeat isassociated with a higher TS gene expression than a double re-peat, resulting in poorer outcome of ALL patients treated with anti-folate methotrexate (MTX).Objective In this study, we determined the incidences of TS andmethylenetetrahydrofolate reductase (MTHFR) polymorphism andethnic variations between Indonesian and Caucasian ALL cellsamples obtained at diagnosis. Furthermore, we determined theinvolvement of TS polymorphisms in MTX sensitivity using athymidilate synthase inhibition assay (TSIA).Methods ALL cell samples were obtained at diagnosis from 101Indonesian and 157 Caucasian children treated with MTX prospec-tively. Genotyping for TS and MTHFR was analyzed by Genescanand Lightcycler. TS polymorphism was determined by PCR assayand MTHFR polymorphism and was analyzed by melting curveanalyses on lightcycler.Results Homozygous TS triple repeats were more than twice ascommon in Indonesian samples (76.3%) than in Caucasian samples(33.1%). Heterozygotes of the MTHFR mutations were seen in 15%of the screened Indonesian samples.Conclusion There are significant ethnic variations in TS generegulatory elements of leukemic cells. A difference was found be-tween the MTX sensitivity and a double or triple repeat in the Cau-casian ALL group. The samples with a triple repeat show a shift intheir distribution towards hypersensitivity to MTX. Further investi-gation on Indonesian samples may give insight in the role of poly-morphisms in MTX sensitivity
Apoptotic cell identification: An in-vivo study during induction treatment of childhood acute lymphoblastic leukemia Pudjo H Widjajanto; AJP Veerman; Sutaryo Sutaryo
Paediatrica Indonesiana Vol 46 No 5 (2006): September 2006
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/pi46.5.2006.195-8

Abstract

Background Acute lymphoblastic leukemia (ALL) in children hashigh cure rate but it can cause death due to the side effects oftreatment or to the disease itself. Thus the evaluation on responseof treatment is important and may predict the prognosis. Sinceapoptosis can be induced by chemotherapy, it is thought that thenumber of leukemic cells that undergo apoptosis may reflect drugsensitivity and cytoreduction rate, thus it may correlate with prog-nosis.Objective To detect apoptotic cells in peripheral blood of childrenwith ALL during the first week of treatment.Methods We conducted a cross sectional study on 58 childrenwith newly diagnosed ALL treated in Department of Child Health,Sardjito Hospital, Yogyakarta. Apoptotic cells were detected onsmears of buffy coat made from peripheral blod and stained withMay-Grunwald Giemsa. The apoptotic cells viewed under light mi-croscope within 12 time points during 7 days after treatment started.Results Apoptotic cells were identified in 3 of 58 patients withindex range of 4.2% to 36.2%.Conclusion Apoptotic cells can be detected in peripheral bloodwith simple method. The explanation of why not all blood smearsviewed showed these cells need further study. It may due to themethods or the apoptotic process itself.
Co-Authors Edy Supriadi IDG Ugrasena Jacqueline Cloos Jan Hendrik Hooijberg Laura Vroling Pudjo H Widjajanto Sutaryo Sutaryo