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D. Sugianto
Department of Pediatrics, Sumber Waras Hospital/Tarumanegara University, Jakarta
Health Professions Medicine & Pharmacology

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Some clinical and epidemiological observations on virologically confirmed dengue hemorrhagic fever T. K. Samsi; H. Wulur; D. Sugianto; C. R. Bartz; R. Tan; A. Sie
Paediatrica Indonesiana Vol 30 No 11-12 (1990): November - December 1990
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/pi30.11-12.1990.293-303

Abstract

This study is a part of a one year prospective study on dengue hemorrhagic fever (DHF) carried out in the Department of Pediatrics, Sumber Waras Hospital in West Jakarta. Viral isolation and serologic analysis for DHF were done by Namru-2 in Jakarta. The subjects were 151 virologically confirmed DHF Patients admitted to the Department of Pediatrics during the period of September 1987 - June 1988, consisting of 82 boys and 69 girls of 6 months - 15 years old. The predominant age group was 5 to 9 years, representing 49. 7% subjects. Dengue virus was isolated from sera during the first 8 days of illness and in 139 (42.1%) during the first. 5 days of illness. Dengue virus type 1, 2, 3 and 4 were isolated from 16.6%, 13.2%, 69.5% and 0 . 7% subjects, respectively. The clinical manifestations revealed no striking differences between dengue 3 and others except for thrombocytopenia and shock. High fever, hemoconcentration and thrombocytopenia on admission was observed in 30.5%, 8,6% and 8.6% of subjects, respectively. Dengue shock syndrome (DSS) were observed in 23 (15.2%) with 3 (2%) fatal cases. Dengue virus serotype 3 was observed in 20 out of 23 DSS cases (86,9%) and all fatal cases were associated with dengue type 2. This study revealed that dengue 3 is the predominant virus circulating during recent epidemics and is associated with more severe clinical manifestation and with a higher incidence rate of living area.
Immunoglobulin M and G in Virologically Confirmed Dengue Hemorrhagic Fever Tatang K. Samsi; H. Wulur; D. Sugianto; C. R. Bartz; R. Tan; A. Sie
Paediatrica Indonesiana Vol 32 No 3-4 (1992): March - April 1992
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (673.383 KB) | DOI: 10.14238/pi32.3-4.1992.65-74

Abstract

Starting from September 1987, a one year prospective study on lgM and lgG in dengue hemorrhagic fever, was carried out at the Department of Pediatrics, Sumber War as Hospital West Jakarta. This report describes the preliminary finding of the study from September 1987 through June 1988. Virus isolation and serologic analysis (HI, lgG and lgM capture ELISA) for DHF were done by NAMRU 2 in Jakarta. The subjects were 151 virologically confirmed DHF patients consisting of 82 boys and 69 girls of 6 months - 15 years old. Serum samples were collected: (I) on the day of admission; (II) on the day of discharge and (Ill) 2 weeks after the first samples. Serum samples I, II, and Ill were collected from respectively 151, 131 and 64 subjects on respectively 3.5 ± 1.7; 8.8 ± 2.7 and 16.8 ± 2.1 days of illness. Positive lgM titer from acute sera was observed in 20% of subjects. A positive correlation between HI -lgM and HI -lgG was also observed. The percentage of positive lgM titers rose with the increase of HI titer, the percentage of positive lgG titer was lower than that of /gM but a sudden increase exceeding that of lgM was observed at the HI liter of 320 and more. This study revealed that HI titer of640 and 1280 were indicators for the primary and secondary dengue infection respectively and lgM capture ELISA can be used as a reliable predictor for DHF even more in fatal cases where only single serum is available.
Co-Authors A. Sie A. Sie C. R. Bartz C. R. Bartz H. Wulur R. Tan R. Tan T. K. Samsi Tatang K. Samsi