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Purnomo Suryantoro Purnomo Suryantoro
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Hemoglobin M-Saskatoon clarified at molecular level by DNA sequencing of the 13-globin gene Purnomo Suryantoro, Purnomo Suryantoro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 30, No 01 (1998)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.476 KB)

Abstract

DNA sequencing of the 0-globin gene was done and clarify the Hemoglobin M-Saskatoon at molecular level.A boy was detected to suffer from 13-thalassemia since three year old. At four years of age, he underwent splenectomy due to severe splenomegaly. At 10 years of age blood sample was withdrawn and enzymatic sequencing of blood lympocyte DNA showed a mutation at Codon 63 (CAT->TAT). Therefore, hemoglobin M-Saskatoon was diagnosed. This mutation was also found in his mother detected by using NLA Ill restriction enzyme which digests the wild type of DNA at the CATG/region. This is the first report to demonstrate sequencing technique identifying hemoglobin M instead of using the biophysical examination of the blood oxygen binding affinity.Key words: /3-thalassemia - hemoglobin M - Saskatoon - DNA sequencing - endonuclease restriction enzyme
Kadar amonia dan urea darah bayi prematur pada usia minggu pertama Purnomo Suryantoro, Purnomo Suryantoro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 01 (1996)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.926 KB)

Abstract

Ammonia and urea blood levels in premature infants during the first week of life were analyzed. Twenty seven blood samples from premature infants at the age of 1, 3 and 7 days were collected. The concentration of ammonia was determined using Amitest meter (TSUGAI), while the urea concentration was measured using regular techniques. Even though the ammonia levels did not exceed 180 pg/d1., the data showed that the critical situation occur in the first three days of life when the ammonia concentration was the highest and urea was also concentrated. This condition releaved in day seven.Key words : ammonia level - urea level - premature baby - enzyme deficiency - hyperammonemia
Formazan ring method: a simple test for screening of glucoce-6-phosphate dehydrogenase (G-6-PD) deficiency in the neonates Purnomo Suryantoro, Purnomo Suryantoro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 02 (1996)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.161 KB)

Abstract

Glucose-6-phosphate dehydrogenase deficiency, is an x-linked inherited disease which is responsible for episodic hemolytic anemia. The incidence correlates with the malarial epidemic in many area like Mediterranean, Africa and Asia including South East Asia.This paper reports a simple, cheap, traumatic, and reliable method screening test called formazan ring developed by Nishiyama. Among 145 new-born infants in this report the low enzyme activity was as high as 9(6.2%) Including 2(1.4%) G-6-PD deficiency.Key words: glucose-6-phosphate dehydrogenase deficiency - x-linked inherited disease - formazan ring test - neonatal screening
Jalannya asthma bronchiale pada anak Purnomo Suryantoro, Purnomo Suryantoro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 9, No 03 (1977)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.344 KB)

Abstract

keywords: asthma bronchiale, syaraf otonom-tidak seimbang, eksema infantil
Hemoglobin M-Saskatoon clarified at molecular level by DNA sequencing of the 13-globin gene Purnomo Suryantoro Purnomo Suryantoro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 30, No 01 (1998)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.476 KB)

Abstract

DNA sequencing of the 0-globin gene was done and clarify the Hemoglobin M-Saskatoon at molecular level.A boy was detected to suffer from 13-thalassemia since three year old. At four years of age, he underwent splenectomy due to severe splenomegaly. At 10 years of age blood sample was withdrawn and enzymatic sequencing of blood lympocyte DNA showed a mutation at Codon 63 (CAT->TAT). Therefore, hemoglobin M-Saskatoon was diagnosed. This mutation was also found in his mother detected by using NLA Ill restriction enzyme which digests the wild type of DNA at the CATG/region. This is the first report to demonstrate sequencing technique identifying hemoglobin M instead of using the biophysical examination of the blood oxygen binding affinity.Key words: /3-thalassemia - hemoglobin M - Saskatoon - DNA sequencing - endonuclease restriction enzyme
Formazan ring method: a simple test for screening of glucoce-6-phosphate dehydrogenase (G-6-PD) deficiency in the neonates Purnomo Suryantoro Purnomo Suryantoro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 02 (1996)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.161 KB)

Abstract

Glucose-6-phosphate dehydrogenase deficiency, is an x-linked inherited disease which is responsible for episodic hemolytic anemia. The incidence correlates with the malarial epidemic in many area like Mediterranean, Africa and Asia including South East Asia.This paper reports a simple, cheap, traumatic, and reliable method screening test called formazan ring developed by Nishiyama. Among 145 new-born infants in this report the low enzyme activity was as high as 9(6.2%) Including 2(1.4%) G-6-PD deficiency.Key words: glucose-6-phosphate dehydrogenase deficiency - x-linked inherited disease - formazan ring test - neonatal screening
Kadar amonia dan urea darah bayi prematur pada usia minggu pertama Purnomo Suryantoro Purnomo Suryantoro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 01 (1996)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.926 KB)

Abstract

Ammonia and urea blood levels in premature infants during the first week of life were analyzed. Twenty seven blood samples from premature infants at the age of 1, 3 and 7 days were collected. The concentration of ammonia was determined using Amitest meter (TSUGAI), while the urea concentration was measured using regular techniques. Even though the ammonia levels did not exceed 180 pg/d1., the data showed that the critical situation occur in the first three days of life when the ammonia concentration was the highest and urea was also concentrated. This condition releaved in day seven.Key words : ammonia level - urea level - premature baby - enzyme deficiency - hyperammonemia
Jalannya asthma bronchiale pada anak Purnomo Suryantoro Purnomo Suryantoro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 9, No 03 (1977)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.344 KB)

Abstract

keywords: asthma bronchiale, syaraf otonom-tidak seimbang, eksema infantil
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