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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Sumberdaya Hayati
Edi Djauhari Purwakusumah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology

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Genetic Variation Analysis of Hevea brasiliensis Genotype Population of In Vitro Micro-Cutting Culture by RAPD Marker MARTIANSYAH, IRFAN; HARIS, NURHAIMI; HUSNIYATI, TATI; PURWAKUSUMAH, EDI DJAUHARI
Jurnal Sumberdaya Hayati Vol. 4 No. 2 (2018)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.4.2.57-62

Abstract

The rubber seeds are insufficient for producing rootstocks to rubber grafting. It can be overcome by an in vitro micro-cutting culture technique developed in the Indonesian Research Institute for Biotechnology and Bioindustry (IRIBB). However, the origin clone of 57 rubber genotypes used as an explant source in vitro micro-cutting culture is not recognized. The study was to investigate the 57 genotypes that came from mixed GT 1, PB 260, and RRIM 600 as parent clones. We investigated using seven primers of Random Amplified Polymorphic DNA (RAPD), i.e., OPA 02, OPA 07, OPA 15, OPB 04, OPC 05, OPC 11, and OPC 20. The qualitative analyzed by electrophoresis 1% gel agarose. A total of 47 DNA fragments produced with an average of 7 fragments per primer. OPA 02 generated of 13 fragments, whereas OPB 04 only one fragment. The DNA fragment pattern shows the presence of polymorphism. The genetic similarity coefficients obtained in the range of 62-96%. The highest genetic similarity (96%) is genotype 70 and 78. It recognized that 42 genotypes from 57 rubber genotypes had the closest relationship with PB 260 clones. Furthermore, six genotypes had a significant growth response as an explant in vitro micro-cutting culture.
Pengaruh Cekaman Kekeringan dan Umur Panen Terhadap Pertumbuhan dan Kandungan Xanthorrhizol Temulawak (Curcuma xanthorrhiza roxb.) Khaerana, ,; Ghulamahdi, Munif; Purwakusumah, Edi Djauhari
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.632 KB) | DOI: 10.24831/jai.v36i3.1383

Abstract

The research was conducted from November 2005 until May 2006 to determine growth and production of the Curcuma xanthorrhiza based on agronomical, physiological variables, and bioactive (xanthorrhizol) content.  The experiment was arranged in a completely randomized design with two factors and three replications.  The first factor consisted of 4 level of drought stress i.e: 100% field capacity (FC) (as control), 50% FC started at 2 weeks before harvesting (wbh),  50% FC started at 4 wbh,  and 50% FC started at 6 wbh.  The second factor comprised of 2 harvest times i.e. 5 months and 7 months.  The result showed that drought stress decreased plant growth and productivity, but increased accumulation of proline.  The harvesting time significantly increased the agronomic character, but did not significantly increase proline content.  The older samples contained more xanthorrhizol than the younger samples.   Key words: Curcuma xanthorrhiza, drought stress, harvesting time, plant growth,  xanthorrhizol.
Co-Authors , Khaerana HARIS, NURHAIMI HUSNIYATI, TATI MARTIANSYAH, Irfan Munif Ghulamahdi