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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Miftahul Ilmiah
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience

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METODE BROMCRESOL GREEN (BCG) DAN BROMCRESOL PURPLE (BCP) PADA SIROSIS HATI YANG MENDAPAT INFUS ALBUMIN Miftahul Ilmiah; Leonita Anniwati; Soehartini Soehartini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1070

Abstract

Albumin infusion is one of the therapeutic options in hypoalbuminemia patients. Serum albumin can be used to determine the albumininfusion therapy, prognosis and monitoring of liver cirrhosis. The time difference in measurement of serum albumin by bromcresol green(BCG) and bromcresol purple (BCP) methods can give different results. Serum albumin examination was done in 20 sera taken fromcirrhosis patients. Serum albumin was then evaluated before treatment, one (1) hour and 24 hours after the patient received an infusionof albumin and examined by bromcresol green (BCG) and bromcresol purple (BCP) methods. The serum albumin level by BCG methodincreased with a coefficient of 0.12 (p-value=0.022) with BCG method before (1.94±0.32 mg/dL) and after one (1) hour (2.06±0.32mg/dL) receiving intravenous albumin. The coefficient of albumin levels before and after 24 hours (2.12±0.38 mg/dL) was 0.18 (pvalue=0.07), whereas the increased levels of serum albumin after one (1) hour and after 24 hours of intravenous albumin, were notsignificant (p-value=0.467). The BCP method showed that serum albumin before, after one (1) hour and after 24 hours receivingintravenous albumin were 1.68±0.36 mg/dL, 1.87±0.36 mg/dL and 2.12±0.63 mg/dL respectively. The albumin levels showed asignificant increase before and after one (1) hour infusion of albumin (p-value=0.00), both levels shown before and after 24 hours(p-value=0.001), as well as one (1) hour and 24 hours after receiving intravenous albumin (p-value=0.04). The results of this studyshowed that increased serum albumin by BCG method could be detected after 1 (one) hour, whereas by BCP method could only be detectedafter 24 hours receiving intravenous albumin.
DETECTION OF MYCOBACTERIUM TUBERCULOSIS WITH TB ANTIGEN RAPID TEST IN PULMONARY TUBERCULOSIS PATIENTS WITH FOUR TYPES OF SPUCTUM SAMPLE PREPARATION Miftahul Ilmiah; IGAA. Putri Sri Rejeki; Betty Agustina Tambunan
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1132

Abstract

Pemeriksaan mikroskopis langsung untuk mendiagnosis TB memiliki banyak keterbatasan. TB Ag Rapid Test Device merupakanmetode pemeriksaan ICT TB yang mendeteksi antigen yang disekresi khas dari Regions of Difference (RD) M.tuberculosis. Penelitiansebelumnya menunjukkan uji sering tidak berjalan dengan baik. Sampel penelitian adalah 30 dahak BTA positif dengan pemeriksaanmikroskopis langsung. Dahak diberikan 4 perlakuan berbeda yaitu: perlakuan rutin sesuai tata langkah perangkat; penambahan0,5 mL NALC 2,5% dan dahak dikocok sesuai tata langkah perangkat; perlakuan vorteks dilanjutkan pemusingan 10.000g suhu 4°C;penambahan 0,5 mL NALC 2,5%, vorteks dilanjutkan pemusingan 10.000g suhu 4°C. Hasil pemeriksaan antigen M.tuberculosis sebagaiberikut: perlakuan kesatu 43,3% positif, perlakuan kedua hasil positif tinggi (96,7%), perlakuan ketiga dan keempat didapatkanhasil positif sebesar 36,7% dan 86,7%. Pemeriksaan Ag menggunakan vorteks-pemusingan dengan pemeriksaan rutin menunjukkankesesuaian sebesar 86,6% (33,3% positif dan 53,5% negatif) dengan nilai Kappa 0,724 (p<0,0001). Perlakuan 2 (penambahan0,5 mL NALC 2,5%) dengan perlakuan 4 (penambahan NALC 2,5%-vorteks-pemusingan) menunjukkan kesesuaian sebesar 90% (86,7%positif dan 3,3% negatif) dengan nilai Kappa 0,366 (p=0,010). Pemberian pretreatment 0,5 mL NALC 2,5% dapat digunakan untukmeningkatkan hasil positif kit TB Ag Rapid Test Device untuk mendiagnosis tuberkulosis paru.
Co-Authors Betty Agustina Tambunan IGAA. Putri Sri Rejeki Leonita Anniwati Soehartini Soehartini