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All Journal Berkala Penelitian Hayati
Ekachai Chukeatirote
School of Science Mae Fah Luang University 333 Moo 1, Tambon Tasud Muang District, Chiang Rai, Thailand-57100
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Characterization of protease-producing bacteria isolated from terasi Novi Arfarita; Ekachai Chukeatirote
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.549 KB) | DOI: 10.23869/89

Abstract

Total of 117 bacterial strains were isolated from terasi samples and 69% of isolates (71) could perform distinctive proteolytic activity that related to the ability to produce protease enzymes. Their proteolytic activity was further tested using spot incubation technique. Strain S4-5 has shown the highest activity then was selected for further tests in this study. Gram staining test showed that S4-5 is gram positive bacteria and able to grow under an aerobic condition. Based on API biochemical profiles, S4-5 strain bacteria was Bacillus licheniformis. Similarity test of genome sequence among Bacillusspecies from gene bank (EMBL Sequence Version) with Bacillusspp., strain S4-5 had similarity with Bacillus licheniformis genome. The optimal pH of this strain was 6 whereas the optimum temperature for Bacillus licheniformis strain S4-5 was 37°C.
Co-Authors Novi Arfarita