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All Journal Berkala Penelitian Hayati Biodiversitas Journal of Biological Diversity
R. Susanti
Department of Biology, Faculty of Mathematics and Natural Sciences, Semarang State University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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THE PREVALENCE OF H5N1 INFLUENZA VIRUS ON POULTRY AT TRADITIONAL MARKET IN SEMARANG, INDONESIA R. Susanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 2 (2015): June 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.102 KB) | DOI: 10.23869/101

Abstract

Traditional market is a potential source of the spread of avian influenza virus. This research is aimed to determine the prevalence of avian influenza virus (AIV) subtype H5N1 on poultry sold in traditional market in Semarang City. Fifty-five poultry samples sold in six traditional markets in Semarang City; i.e. Karangayu market, Mangkang market, Gunungpati market, Rejomulyo market, Gayamsari market and Karimata market taken from its cloaca swab. Further, cloacal swab samples grown in pathogen-free chicken embryos aged nine days. Then, it incubated for four days at 37 °C. Allantoic fluid then collected and tested to agglutinate red blood cells. RNA extracted from the samples showed haemagglutination activity. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) applied to identify the subtype of AIV using the specific primer H5 and N1. The PCR product resulted from RT-PCR then visualized using electrophoresis. The results showed that four AIV subtype H5N1 isolates have successfully isolated from cloacal swab samples (7.27%) with the distribution of 2 isolates were from Mangkang Market (28.57%), 1 isolate was from Rejomulyo Market (7.69%) and 1 isolate was from Karimata Market. The prevalence of AIV subtype H5N1 was 28.57% in Mangkang market, 7.69% in Rejomulyo market and 9.09% in Karimata market. The prevalence values of each species were 7.89% in chicken and 9.09% in the wild duck. According to this result, poultry sold in traditional market in Semarang have a potential source of AIV subtype H5N1.
Polymorphic sequence in the ND3 region of Java endemic Ploceidae birds mitochondrial DNA R. SUSANTI
Biodiversitas Journal of Biological Diversity Vol. 12 No. 2 (2011)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d120203

Abstract

Susanti R (2011) Polymorphic sequence in the ND3 region of Java endemic Ploceidae birds mitochondrial DNA. Biodiversitas 12: 70- 75. As part of biodiversity, Ploceidae bird family must be kept away from extinction and degradation of gene-diversity. This research was aimed to analyze ND3 gene from mitochondrial DNA of Java Island endemic of Ploceidae bird. Each species of Ploceidae birds family was identified based on their morphological character, then the blood sample was taken from the birds nail vein. DNA was isolated from blood using Dixit method. Fragment of ND3 gene was amplified using PCR method with specific primer pairs and sequenced using dideoxy termination method with ABI automatic sequencer. Multiple alignment of ND3 nucleotide sequences were analyzed using ClustalW of MEGA-3.1 program. Estimation of genetic distance and phylogenetic tree construction were analyzed with Neighbor-Joining method and calculation of distance matrix with Kimura 2 –parameter. The result of Java Island endemic of Ploceidaebird family exploration showed that Erythrura hyperythra and Lonchura ferruginosa can not be found anymore in nature, but the Lonchura malacca that are not actually Java island endemic was also found. Nucleotide sequence of mitochondrial ND3 gene of Ploceidae bird family showed a quite high polymorphism, with 122 substitutions from 334 nucleotides analyzed. Phylogenetic tree of nucleotide sequence of Ploceidae bird family formed 2 clusters. One cluster consisted of the Ploceus hypoxanthus, Ploceus philippinus, Ploceus manyar and Passer montanus, and the others species were included in the second cluster. ND3 gene sequence data from this Ploceidae family need to be analyzed further to see possible relationship with a particular phenotype.
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